Apexification with mineral trioxide aggregate plug in the upper anterior teeth: Presentation of three clinical cases

AbstrAct Introduction: the aim is to describe the treatment of three immature permanent incisors associated to apical periodontitis, based on the placement of an apical mineral trioxide aggregate (MtA) plug for apexification. case series: Apexification was carried out by opening the pulp chamber, with debridement of the canal following anesthesia and isolation of the tooth. the canal was filled with pure calcium hydroxide in powder form, dissolved in saline solution for one week, and the aperture was sealed with IrM (Dentsply, Germany). One week later, the calcium hydroxide was removed and an MtA apical plug was positioned, sealing with a humid cotton pellet and IrM (Dentsply, Germany). After setting of the MtA, conventional endodontic treatment was carried out using gutta-percha, with definitive restoration of the tooth. conclusion: All three cases, treated with MtA showed complete apical repair with rounding of the apex at radiographi

GWAS of Post-Orthodontic Aggressive External Apical Root Resorption Identified Multiple Putative Loci at X-Y Chromosomes.

Personalized dental medicine requires from precise and customized genomic diagnostic. To conduct an association analysis over multiple putative loci and genes located at chromosomes 2, 4, 8, 12, 18, X, and Y, potentially implicated in an extreme type of external apical root resorption secondary to orthodontic forces (aEARR). A genome-wide association study of aEARR was conducted with 480 patients [ratio~1:3 case/control]. Genomic DNA was extracted and analyzed using the high-throughput Axiom platform with the GeneTitan® MC Instrument. Up to 14,377 single nucleotide polymorphisms (SNPs) were selected at candidate regions and clinical/diagnostic data were recorded. A descriptive analysis of the data along with a backward conditional binary logistic regression was used to calculate odds ratios, with 95% confidence intervals [p < 0.05]. To select the best SNP candidates, a logistic regression model was fitted assuming a log-additive genetic model using R software [p < 0.0001]. In this sample the top lead genetic variants associated with aEARR were two novel putative genes located in the X chromosome, specifically, STAG 2 gene, rs151184635 and RP1-30E17.2 gene, rs55839915. These variants were found to be associated with an increased risk of aEARR, particularly restricted to men [OR: 6.09; 95%CI: 2.6-14.23 and OR: 6.86; 95%CI: 2.65-17.81, respectively]. Marginal associations were found at previously studied variants such as SSP1: rs11730582 [OR: 0.54; 95%CI: 0.34-0.86; p = 0.008], P2RX7: rs1718119 [OR: 0.6; 95%CI: 0.36-1.01; p = 0.047], and TNFRSF11A: rs8086340 [OR: 0.6; 95%CI: 0.38-0.95; p = 0.024]), found solely in females. Multiple putative genetic variants located at chromosomes X and Y are potentially implicated in an extreme phenotype of aEARR. A gender-linked association was noted.This research was funded by the European Orthodontic Society Research Award, 2017.S

Thermochemical properties of two benzimidazole derivatives: 2-Phenyl- and 2-benzylbenzimidazole

The standard (p° = 0.1 MPa) molar enthalpy of formation for gaseous 2-phenylbenzimidazole (2-PhBIM) and 2-benzylbenzimidazole (2-BzBIM) were derived from the standard molar enthalpies of combustion, at T = 298.15 K, measured by static bomb calorimetry, and the standard molar enthalpy of sublimation, at T = 298.15 K, measured by Calvet microcalorimetry in the case of 2-phenylbenzimidazole or derived from the variation of the vapour pressures, determined by the Knudsen effusion technique, with temperatures between (393 and 412) K for 2-benzylbenzimidazole. Heat capacities, in the temperature ranges from (268.15 to 322.10) K for 2-phenylbenzimidazole and (270.15 to 316.02) K for 2-benzylbenzimidazole, were also measured with a differential scanning calorimeter. ΔcHm°(cr)/kJ·mol-1ΔcrgHm°(T=298.15K)/ kJ·mol-12-Phenylbenzimidazole (2-PhBIM)-6679.8 ± 0.9123.0 ± 1.72-Benzylbenzimidazole (2-BzBIM)-7327.1 ± 4.0136.2 ± 0.5 © 2005 Elsevier Ltd. All rights reserved.Peer Reviewe

Sol–gel synthesis of Mg(OH)2 and Ca(OH)2 nanoparticles: a comparative study of their antifungal activity in partially quaternized p(DMAEMA) nanocomposite films

The evaluation of the antifungal activity of Mg(OH)2 and Ca(OH)2 nanoparticles (NPs), synthesized by sol–gel method and their mixtures at different concentrations, is reported. The antifungal activity of the hydroxide NPs was studied using Aspergillus niger and Penicillium oxalicum isolated from stone surfaces. These model organisms were selected due to their ability to grow on outdoor and indoor climates and their significant impact on human health. Moreover, the antifungal activity of Mg(OH)2 and Ca(OH)2 NPs dispersed in positively charged polymeric matrices based on partially quaternized poly(2-(dimethylamino ethyl) methacrylate) (pDMAEMA) was studied. With respect to the morphology, particle size, and textural properties of the NPs, the mixtures of Mg–Ca hydroxides revealed a uniform and smaller particle size, along with a greater surface area, as compared to pristine Ca(OH)2 NPs. However, the Ca(OH)2 and a mixture of Mg(OH)2 and Ca(OH)2 (10:90 weight ratio) NPs, showed an enhanced growth inhibition of A. niger and P. oxalicum, suggesting that the effect of particle size on the antifungal activity would not be a preponderating factor. In addition, improved antifungal properties against A. niger and P. oxalicum were detected in composite coatings based on hydroxide NPs dispersed in quaternized p(DMAEMA-co-METAI). The use of these systems might provide promising composite materials with potential antifungal properties for various applications.This study was financially supported by the National Council for Science and Technology (Consejo Nacional de Ciencia y Tecnología [CONACYT, Mexico]) of the “Fronteras de la Ciencia No. 138” project and by the Community of Madrid under the “Climortec”, BIA2014−53911-R, “Geomaterials 2” Programme (S2013/MIT_2914), and Multimat Challenge (S2013/MIT-2862). A.S.-F. would like to gratefully acknowledge the financial support of Santander Universidades through “Becas Iberoamérica Jóvenes Profesores e Investigadores, España 2015” scholarship program. C.G.-S., R.Y.-M., and U.S.S. thank CONACYT and the Deutscher Akademischer Austauschdienst (DAAD, Germany) for financial support within the framework of the funding program for international mobility PROALMEX 2015 (CONACyT project: 267752 and DAAD project: 57271725). C.G.-S. and U.S.S. also thank the Deutsche Forschungsgemeinschaft (DFG, Germany) for financial support for this research under the scheme of the grant SFB-1278 “PolyTarget” project B02.Peer reviewe

Psychometric properties of the Weight Locus of Control Scale (MWLCS): study with Spanish individuals of diferent anthropometric nutritional status

Introduction The Multidimensional Weight Locus of Control Scale (MWLCS) measures a person’s beliefs regarding the locus of control or lack of locus of control over his/her body weight. Purpose We aim to evaluate the factorial structure and psychometric properties of the MWLCS with Spanish normal weight, overweight and obese samples. Methods The research was carried out in two diferent studies. The frst included a sample of 140 normal weight participants, selected out of a 274 sample recruited with an online survey. Study 2 was carried out in a sample of 633 participants recruited from the PREDIMED-Plus study. Out of them, 558 participants fulflled the weight criteria and were categorized into: overweight (BMI 25 − <29.99; N=170), obese class I (BMI 30 − <34.99; N=266), and obese class II (BMI 35 −<39.99; N=122). Exploratory (EFA) and confrmatory (CFA) factor analyses were used to evaluate the factor structure of the MWLCS, and reliabilities and Spearman’s correlations were estimated. Invariance measurement was tested across the three subgroups of weight in Study 2. Results A three-factor structure indicating weight locus of control factors (internal, chance, and powerful others) was supported, both via EFA in the normal weight sample and CFA in the overweight and obese samples. In the normal weight sample, the powerful others dimension was positively related to BMI and the dimensions of the Dutch Eating Behaviors Questionnaire. Additionally, the scale showed evidence of scalar invariance across the groups with diferent weight conditions. Conclusions This scale seems to be a psychometrically appropriate instrument and its use is highly recommended when designing interventions for overweight or obese individuals. Level of evidence Level V, descriptive study

Successful engraftment of gene-corrected hematopoietic stem cells in non-conditioned patients with Fanconi anemia

International audienceFanconi anemia (FA) is a DNA repair syndrome generated by mutations in any of the 22 FA genes discovered to date1,2. Mutations in FANCA account for more than 60% of FA cases worldwide3,4. Clinically, FA is associated with congenital abnormalities and cancer predisposition. However, bone marrow failure is the primary pathological feature of FA that becomes evident in 70–80% of patients with FA during the first decade of life5,6. In this clinical study (ClinicalTrials.gov, NCT03157804; European Clinical Trials Database, 2011-006100-12), we demonstrate that lentiviral-mediated hematopoietic gene therapy reproducibly confers engraftment and proliferation advantages of gene-corrected hematopoietic stem cells (HSCs) in non-conditioned patients with FA subtype A. Insertion-site analyses revealed the multipotent nature of corrected HSCs and showed that the repopulation advantage of these cells was not due to genotoxic integrations of the therapeutic provirus. Phenotypic correction of blood and bone marrow cells was shown by the acquired resistance of hematopoietic progenitors and T lymphocytes to DNA cross-linking agents. Additionally, an arrest of bone marrow failure progression was observed in patients with the highest levels of gene marking. The progressive engraftment of corrected HSCs in non-conditioned patients with FA supports that gene therapy should constitute an innovative low-toxicity therapeutic option for this life-threatening disorder