Parameterisation of flows and pollutant transport over idealised urban roughness

No. J7.2Atmospheric flow over urban areas basically is a type of turbulent flow over roughness. The flows and pollutant transport process, especially at the lower part of the boundary layer (BL), is strongly modified due to the presence of building geometry. The aerodynamic resistance exerted by the surface roughness reduces the mean velocity in the lower BL but enhances the turbulence intensity. Moreover, the near-wall impingement structures over rough surfaces are attributed to the flow dynamics aloft, leading to increasing aerodynamic resistance and BL depth. However, the dependence on surface morphology and BL depth is not yet well understood. There is ...postprin

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.

Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of heterologous counter-selection markers, here we explore novel DNA integration strategies that do not employ them, and instead exploit (i) activation or inactivation of genes leading to a selectable phenotype, and (ii) asymmetrical regions of homology to control the order of recombination events. We focus here on the industrial biofuel-producing bacterium Clostridium acetobutylicum, which previously lacked robust integration tools, but the approach we have developed is broadly applicable. Large sequences can be delivered in a series of steps, as we demonstrate by inserting the chromosome of phage lambda (minus a region apparently unstable in Escherichia coli in our cloning context) into the chromosome of C. acetobutylicum in three steps. This work should open the way to reliable integration of DNA including large synthetic constructs in diverse microorganisms. © 2011 The Author(s)

Wetland Habitat Change in the Shenzhen River Cross-boundary Catchment, China

Session: River Ecology (C2A): Beneath the Surfacepublished_or_final_versio

Radio polarization observations of the snail: a crushed pulsar wind nebula in G327.1–1.1 with a highly ordered magnetic field

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WIJAM: a mobile collaborative improvisation platform under Master-players Paradigm

Music jamming is an extremely difficult task for musical novices. Trying to extend this meaningful and highly enjoyable activity to a larger recipient group, we present WIJAM, a mobile application for an ad-hoc group of musical novices to perform improvisation along with a music master. In this master-players' paradigm, the master offers a music backing, orchestrates the musical flow, and gives feedbacks to the players; the players improvise by tapping and sketching on their smartphones. We argue that this paradigm can be a significant contribution to the possibility of music playing by a group of novices with no instrumental training leading to decent musical results.published_or_final_versio

Reliability and validity of alternate step test times in subjects with chronic stroke

OBJECTIVE: (i) To investigate the intra-rater, inter-rater and test-retest reliability and minimal detectable change of the Alternate Step Test (AST) when assessing people with chronic stroke. (ii) To quantify the correlation between AST times and stroke-specific impairments. DESIGN: Cross-sectional study. SETTING: University-based rehabilitation centre. PARTICIPANTS: A convenience sample of 86 participants: 45 with chronic stroke, and 41 healthy elderly subjects. METHODS: The AST was administered along with the Fugl-Meyer Lower Extremity Assessment (FMA-LE), the Five Times Sit-To-Stand Test (FTSTS), limits of stability (LOS) measurements, Berg Balance Scale (BBS) scores, Chinese-translated Activities-specific Balance Confidence Scale (ABC-C) ratings, and the Timed “Up and Go” test (TUG). RESULTS: Excellent intra-rater, inter-rater and test-retest reliability were found, with a minimal detectable change of 3.26 s. AST times were significantly associated with FMA-LE assessment, FTSTS times, LOS in the forward and backward directions and to the affected side, BBS ratings and TUG times. CONCLUSION: AST time is a reliable assessment tool that correlates with different stroke-specific impairments in people with chronic stroke.published_or_final_versio

Recent Advances in Animal and Human Pluripotent Stem Cell Modeling of Cardiac Laminopathy

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Modeling Treatment Response for Lamin A/C Related Dilated Cardiomyopathy in Human Induced Pluripotent Stem Cells.

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Epidemiological and Evolutionary Dynamics of Influenza B Viruses in Malaysia, 2012-2014

Epidemiological and evolutionary dynamics of influenza B Victoria and Yamagata lineages remained poorly understood in the tropical Southeast Asia region, despite causing seasonal outbreaks worldwide. From 2012-2014, nasopharyngeal swab samples collected from outpatients experiencing acute upper respiratory tract infection symptoms in Kuala Lumpur, Malaysia, were screened for influenza viruses using a multiplex RT-PCR assay. Among 2,010/3,935 (51.1%) patients infected with at least one respiratory virus, 287 (14.3%) and 183 (9.1%) samples were tested positive for influenza A and B viruses, respectively. Influenza-positive cases correlate significantly with meteorological factors-total amount of rainfall, relative humidity, number of rain days, ground temperature and particulate matter (PM10). Phylogenetic reconstruction of haemagglutinin (HA) gene from 168 influenza B viruses grouped them into Yamagata Clade 3 (65, 38.7%), Yamagata Clade 2 (48, 28.6%) and Victoria Clade 1 (55, 32.7%). With neuraminidase (NA) phylogeny, 30 intra-clade (29 within Yamagata Clade 3, 1 within Victoria Clade 1) and 1 inter-clade (Yamagata Clade 2-HA/Yamagata Clade 3-NA) reassortants were identified. Study of virus temporal dynamics revealed a lineage shift from Victoria to Yamagata (2012-2013), and a clade shift from Yamagata Clade 2 to Clade 3 (2013-2014). Yamagata Clade 3 predominating in 2014 consisted of intra-clade reassortants that were closely related to a recent WHO vaccine candidate strain (B/Phuket/3073/2013), with the reassortment event occurred approximately 2 years ago based on Bayesian molecular clock estimation. Malaysian Victoria Clade 1 viruses carried H274Y substitution in the active site of neuraminidase, which confers resistance to oseltamivir. Statistical analyses on clinical and demographic data showed Yamagata-infected patients were older and more likely to experience headache while Victoria-infected patients were more likely to experience nasal congestion and sore throat. This study describes the evolution of influenza B viruses in Malaysia and highlights the importance of continuous surveillance for better vaccination policy in this region.published_or_final_versio