Chemical composition and antimicrobial activity of Glebionis coronaria (L.) Cass. ex Spach essential oil

Glebionis coronaria (L.) Cass. ex Spach (syn. Chrysanthemum coronarium L.) is a member of the Asteraceae plant family which has extensive edible, folk medicinal, insecticidal uses. Previously, an essential oil of flowerheads of C. coronarium from Spain was reported to contain camphor (29.2%), α-pinene (14.8%), β-pinene (9.5%), and lyratyl acetate (9.8%). The oil was shown to possess significant antifungal activity [1]. Also, the essential-oil composition and antimicrobial properties of C. coronarium from Ukraine were investigated. The major constituents were found to be chrysanthemyl acetate (24.4%), chrysanthemol (21.8%), chrysanthenyl acetate (7.6%), camphor (7.3%), β-farnesene (5.9%), and α-bisabolol (5.6%). An ethanolic extract of the plant showed antimicrobial activity against Gram-positive (S. aureus) bacteria. [2]. Biological activities of the essential oil of C. coronarium from Jordan were also reported. The essential oil showed a significant antimicrobial activity against Gram-positive bacteria. Also, the oil showed moderate antioxidant activity, weak acetylcholinesterase-inhibitory and potent antiproliferative activities [3]. In the current study, the essential oil of the aerial parts of G. coronaria was obtained by hydrodistillation (3 h). The essential-oil composition was analyzed by means of gas chromatography-mass spectrometry (GC-MS). The main components of the essential oil from the aerial parts were capillene (54.5%) and caryophyllene oxide (9.8%). The current composition differed from the previously reported ones. Thus, G. coronaria originating from Istanbul belongs to a new chemotype of this species. Additionally, the antimicrobial activity of the oil was investigated against Gram-negative (Escherichia coli DH5α) and Gram-positive (Staphylococcus aureus) bacteria. The essential oil showed a growth-inhibitory activity against E. coli (53.3%) and S. aureus (17.1%), tested at 75 mg/mL. The antimicrobial effects of the essential oil were more pronounced against Gram-negative bacteria

Candida auris: Microbiological Characteristics and Laboratory Diagnosis of the Hidden Pathogen

© 2022 Galenos Publishing House. All right reserved.Candida auris was first isolated in Japan in 2009. It has since been reported in more than 25 countries worldwide. This species, which can cause invasive infections with high mortality rates, is difficult to identify with traditional biochemical methods. C. auris can be transmitted among hospitalized patients by colonizing the skin. Resistance to fluconazole and amphotericin B is common in this species, and resistance to echinocandins, which are preferred in the treatment in some countries, is seen to develop. Studies have shown that resistance to antifungals is acquired, not intrinsic, and primary resistance mechanisms against echinocandins and azoles have been identified. This species is difficult to control because of its multidrug resistance, even the emergence of pan-resistant strains, misidentification with conventional methods in clinical microbiology laboratories, and long-term persistence in the hospital environment. In order to prevent infections with infection control measures, advanced laboratory methods that allow accurate diagnosis is needed. In addition, new antifungal agents should be researched and included in practice in order to ensure success in treatment. In this short review, current information about the epidemiology, infections, microbiological features and laboratory diagnosis of C. auris is mentioned

GENE MUTATION PATTERNS OF RIFAMPICIN IN MULTIDRUG- RESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX STRAINS

Objective: The aim of this study was to evaluate the GenoType (R) MTBDRplus test for detection of rifampicin (RIF) resistance in MDR Mycobacterium tuberculosis complex strains

Bacterial Dynamics of Hardaliye, a Fermented Grape Beverage, Determined by High-throughput Sequencing

Hardaliye is a traditional beverage produced by fermenting red grapes with mustard seeds and sour cherry leaves in the Thrace region of Turkey. Few studies have been conducted that have determined the microorganisms responsible for hardaliye fermentation, and those that have are limited to lactic acid bacteria (LAB) using culture-dependent techniques. This study aims to determine the bacterial dynamics of hardaliye fermentation using a culture-independent approach, high-throughput sequencing of 16S rRNA amplicons. Hardaliye was produced using the traditional method, and samples were taken and analyzed on days 0, 2, 4, 6, and 10 of fermentation. During the fermentation period, the pH decreased from 3.65 to 3.23. Amplicon sequencing showed that bacterial diversity was highest at 2 d, and lowest at 10 d, the final day. Although Enterobacteriaceae was the most dominant family at 0 and 2 d, Acetobacteriaceae, specifically Gluconobacter frateurii, became dominant with ~50% relative abundance at 4 d, and increased its abundance to >98% at 6 and 10 d. Although a slight increase in the relative abundance of ~1% (0 d) to ~5% (4 d) was observed in LAB, their presence was limited. This study showed that acetic acid bacteria should not be overlooked in hardaliye fermentation

Measurement of Advanced Glycation End Products Could Be Used as an Indicator of Unhealthy Nutrition for Colorectal Cancer Risk

The main culprit behind most cancers is the accumulation of reactive oxygen species. Glyoxal (GO) and methylglyoxal (MGO) are reactive intermediates created by food processing and they are precursors of advanced glycation end products (AGE) that cause glycative stress. We aimed to evaluate the relationship between AGE levels of healthy volunteers and treatment-naive patients diagnosed with colorectal cancer. The study consisted of patients diagnosed with colorectal cancer and healthy volunteers who underwent routine colonoscopy. The study was conducted with a total of 42 cases, 47.6% (n = 20) female. The ages of the participants in the study ranged from 41 to 82 years, and the mean was 60.57 +/- 10.78 years. The GO and MGO values of the patient group were found to be significantly higher than those of the control group (p = 0.007, p = 0.001, respectively). The risk of colorectal cancer was 22 and 57 times higher in individuals with GO and MGO values above 1.25 mu g/mL and 0.0095 mu g/mL, respectively. The blood AGE level is closely related to diet, and it can be decreased through the appropriate improvement of diet. Thus, the measurement of AGE can be used to predict whether a person's nutrition is healthy or unhealthy and prevent increased risk of colorectal cancer