Interfacial adhesion of laser clad functionally graded materials

Specially designed samples of laser clad AlSi40 functionally graded materials (FGM) are made for evaluating the interfacial adhesion. To obtain the interfacial bond strength notches are made right at the interface of the FGMs. In-situ microstructural observations during straining in a field-emission gun environmental scanning electron microscopy reveal different failure modes of the FGMs and substrate. Mapping of strain fields using digital imaging correlation shows a gradual transition of deformation over the interface region and softening effects in the heat-affected zones of the FGM tracks. The strengthening of the FGM is dominated by the size of the Al halos around the particles, in accordance with a dislocation pile-up model.

Laser melt injection in aluminum alloys: On the role of the oxide skin

In this paper the method of laser melt injection of SiC particles into an aluminum substrate is investigated both experimentally and theoretically. An extremely small operational parameter window was found for successful injection processing. It is shown that the final injection depth of the particles is controlled mainly by the temperature of the melt pool rather than by the particle velocity. A theoretical model that takes into account the wetting behavior and the particle penetration processes is developed on the basis of the observed particle velocity, thickness and area fraction of oxide skin that partially covers the surface of the heated aluminum melt pool. The model reveals the role of the oxide skin: it is relatively strong at low temperature and acts as a severe barrier for the injection process. It was found that preheating the aluminum substrate results in a higher temperature of the melt pool and partial dissolution of the oxide skin, through which the injected particles are able to penetrate

Microstructural control of TiC/a-C nanocomposite coatings with pulsed magnetron sputtering

In this paper, we report some striking results on the microstructural control of TiC/a-C nanocomposite coatings with pulsed direct current (DC) magnetron sputtering. The interface morphology and microstructure evolution as a function of pulse frequency and duty cycle were scrutinized using atomic force microscopy, scanning electron microscopy and high-resolution transmission electron microscopy techniques. It is shown that, with increasing pulse frequency, the nanocomposite coatings exhibit evolutions in morphology of the growing interface from rough to smooth and in the microstructure from strongly columnar to fully columnar-free. In addition, the smoothly growing interface favors the formation of a tailor-made multilayered nanocomposite structure. The fundamental mechanisms are analyzed with the assistance of plasma diagnostic experiments. Ion mass/energy spectrometry measurements reveal that, depending on the frequency and duty cycle of DC pulses, pulsing of the magnetrons can control the flux and energy distribution of Ar+ ions over a very broad range for concurrent impingement on the growing interface of deposited coatings, in comparison with DC sputtering. The significantly enhanced energy flux density is thought to be responsible for the "adatom transfer" in interface smoothening and thus the restraint of columnar growth. (C) 2007 Acta Materialia, Inc. Published by Elsevier Ltd. All rights reserved.</p

Evaluation of interface adhesion of hot-dipped zinc coating on TRIP steel with tensile testing and finite element calculation

In this work, a methodology for the determination of the interface adhesion strength of zinc coating on TRIP steel is present. This method consists of a conventional tensile test in combination with finite element calculation. The relation between the average interface crack length and the applied tensile stress is determined on the partially delaminated coating with in-situ tensile test. The delamination process of zinc coating on steel substrate is simulated by using a two-grain finite element model with different interface adhesion strengths. By comparing the experimental observation with the finite element calculations, the interface adhesion strength is estimated. The interface adhesion strength of the zinc coating on transformation induced plasticity (TRIP) steel is found as high as 160 MPa. The influences of microstructural parameters of zinc coating including zinc grain orientation and grain size on the delamination behavior of the zinc coating are also analyzed with the finite element model

Subversion of actin dynamics by EspM effectors of attaching and effacing bacterial pathogens

Rho GTPases are common targets of bacterial toxins and type III secretion system effectors. IpgB1 and IpgB2 of Shigella and Map of enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli were recently grouped together on the basis that they share a conserved WxxxE motif. In this study, we characterized six WxxxE effectors from attaching and effacing pathogens: TrcA and EspM1 of EPEC strain B171, EspM1 and EspM2 of EHEC strain Sakai and EspM2 and EspM3 of Citrobacter rodentium. We show that EspM2 triggers formation of global parallel stress fibres, TrcA and EspM1 induce formation of localized parallel stress fibres and EspM3 triggers formation of localized radial stress fibres. Using EspM2 and EspM3 as model effectors, we report that while substituting the conserved Trp with Ala abolished activity, conservative Trp to Tyr or Glu to Asp substitutions did not affect stress-fibre formation. We show, using dominant negative constructs and chemical inhibitors, that the activity of EspM2 and EspM3 is RhoA and ROCK-dependent. Using Rhotekin pull-downs, we have shown that EspM2 and EspM3 activate RhoA; translocation of EspM2 and EspM3 triggered phosphorylation of cofilin. These results suggest that the EspM effectors modulate actin dynamics by activating the RhoA signalling pathway

Effect of lubricants and sliding conditions on the tribo-performance of SiO2/epoxy composites

The influence of solid lubricants, normal load, sliding speed and water environment on the tribo-performance of solid lubricant-containing SiO2-epoxy composites is investigated. 10 to 15 wt% PTFE concentration yields a low coefficient of friction (CoF) and low wear rate under 60 N load. With increasing PTFE concentration in the composites, the load capacity decreases. Graphite exhibits worse friction and wear performance than PTFE under dry sliding condition, i.e. the CoF increases with a decreasing load in ball-on-disc. A higher concentration of PTFE-containing tribolayer on the worn composite surface is found under a higher load. The CoF and ball wear rate increase with increasing sliding speed under dry sliding, which is accompanied with an increase of the surface temperature of the composite. A high relative humidity is advantageous for a low friction due to adsorbed water molecules that lubricate the sliding couple. Water lubrication leads to a lower wear rate of the PTFE and graphite filled composites, whereas it gives rise to a higher wear rate of the Al2O3 ball due to the lack of protection of the transfer films

Lipid Anti-Lipid Antibody Responses Correlate with Disease Activity in Systemic Lupus Erythematosus

10.1371/journal.pone.0055639PLoS ONE82

Nonlinear Beam Dynamics Experiments: CE-22

This research was sponsored by the National Science Foundation Grant NSF PHY-931478

Ligase 1 is a predictor of platinum resistance and its blockade is synthetically lethal in XRCC1 deficient epithelial ovarian cancers

Rationale: The human ligases (LIG1, LIG3 and LIG4) are essential for the maintenance of genomic integrity by catalysing the formation of phosphodiester bonds between adjacent 5′-phosphoryl and 3′-hydroxyl termini at single and double strand breaks in duplex DNA molecules generated either directly by DNA damage or during replication, recombination, and DNA repair. Whether LIG1, LIG3 and LIG4 can influence ovarian cancer pathogenesis and therapeutics is largely unknown. Methods: We investigated LIG1, LIG3 and LIG4 expression in clinical cohorts of epithelial ovarian cancers [protein level (n=525) and transcriptional level (n=1075)] and correlated to clinicopathological features and survival outcomes. Pre-clinically, platinum sensitivity was investigated in LIG1 depleted ovarian cancer cells. A small molecule inhibitor of LIG1 (L82) was tested for synthetic lethality application in XRCC1, BRCA2 or ATM deficient cancer cells. Results: LIG1 and LIG3 overexpression linked with aggressive phenotypes, platinum resistance and poor progression free survival (PFS). In contrast, LIG4 deficiency was associated with platinum resistance and worse PFS. In a multivariate analysis, LIG1 was independently associated with adverse outcome. In ovarian cancer cell lines, LIG1 depletion increased platinum cytotoxicity. L82 monotherapy was synthetically lethal in XRCC1 deficient ovarian cancer cells and 3D-spheroids. Increased cytotoxicity was linked with accumulation of DNA double strand breaks (DSBs), S-phase cell cycle arrest and increased apoptotic cells. L82 was also selectively toxic in BRCA2 deficient or ATM deficient cancer cells and 3D-spheroids. Conclusions: We provide evidence that LIG1 is an attractive target for personalization of ovarian cancer therapy