Stability of the selfsimilar dynamics of a vortex filament

In this paper we continue our investigation about selfsimilar solutions of the vortex filament equation, also known as the binormal flow (BF) or the localized induction equation (LIE). Our main result is the stability of the selfsimilar dynamics of small pertubations of a given selfsimilar solution. The proof relies on finding precise asymptotics in space and time for the tangent and the normal vectors of the perturbations. A main ingredient in the proof is the control of the evolution of weighted norms for a cubic 1-D Schr\"odinger equation, connected to the binormal flow by Hasimoto's transform.Comment: revised version, 36 page

Diagnosis and Treatment of Irritable Bowel Syndrome: Clinical Recommendations of the Russian Gastroenterological Association and Association of Coloproctologists of Russia

Aim. Current clinical recommendations accentuate current methods for the diagnosis and treatment of irritable bowel syndrome (IBS).Key points. IBS is a functional bowel disorder manifested with recurrent, at least weekly, abdominal pain with the following attributes (any two leastwise): link to defecation, its frequency or stool shape. The symptoms are expected to persist for at minimum three months in a total six-month follow-up. Similar to other functional gastrointestinal (GI) disorders, IBS can be diagnosed basing on the patient symptoms compliance with Rome IV criteria, provided the absence of potentially symptom-causative organic GI diseases. Due to challenging differential diagnosis, IBS can be appropriately established per exclusionem, with pre-examination as follows: general and biochemical blood tests; tissue transglutaminase IgA/IgG antibody tests; thyroid hormones test; faecal occult blood test; hydrogen glucose/ lactulose breath test for bacterial overgrowth; stool test for enteric bacterial pathogens and Clostridium difficile A/B toxins; stool calprotectin test; abdominal ultrasound; OGDS, with biopsy as appropriate; colonoscopy with biopsy. The IBS sequence is typically wavelike, with alternating remissions and exacerbations often triggered by psychoemotional stress. Treatment of IBS patients includes dietary and lifestyle adjustments, various-class drug agents prescription and psychotherapeutic measures.Conclusion. Adherence to clinical recommendations can facilitate timely diagnosis and improve medical aid quality in patients with different clinical IBS variants

The Russian consensus on the diagnosis and treatment of chronic pancreatitis: Enzyme replacement therapy

The Russian consensus on the diagnosis and treatment of chronic pancreatitis has been prepared on the initiative of the Russian Pancreatology Club to clarify and consolidate the opinions of Russian specialists (gastroenterologists, surgeons, and pediatricians) on the most significant problems of diagnosis and treatment of chronic pancreatitis. This article continues a series of publications explaining the most significant interdisciplinary consensus statements and deals with enzyme replacement therapy

Разработка методики получения дермального внеклеточного матрикса

Despite advancements in modern surgery in the treatment of cutaneous injuries, the search for new methods of ensuring faster and more effective wound healing appears especially urgent today. Tissue engineering is undoubtedly of interest when it comes to developing such technologies. Objective: to determine the optimal protocol for obtaining a decellularized dermal matrix scaffold for subsequent development of tissue-engineered skin. Materials and methods. One Landrace piglet was used as the experimental animal. After preliminary skin treatment with dermatome, 0.3 cm thick samples were taken. Two decellularization protocols were considered: protocol No. 1 was based on the use of Triton X-100 and deoxycholate, protocol No. 2 was only based on deoxycholate. There were 5 processing cycles in total for the 2 protocols. After treatment, acellular matrix scaffolds were examined through histological analysis and quantitative determination of DNA concentration. Next, static recellarization of the matrix scaffolds was carried out with porcine dermal fibroblasts. After that, the matrix scaffolds were tested for cytotoxicity using XTT test and differential staining test to differentiate between live and dead cells. Results. Comparative analysis of the two protocols for porcine dermis decellularization showed that both protocols effectively remove cells and nuclear material, while maintaining the architectonics of the intercellular substance intact, since fibrous structures are not destroyed. But when assessing the biocompatibility of matrix scaffolds based on analysis of cell viability according to data obtained from XTT test and cell–matrix adhesion, the matrix scaffold processed under protocol No. 1, shows advantages. Conclusion. In this study, a decellularization protocol based on Triton X-100 and deoxycholate was noted. The results obtained mark the first stage towards developing a tissue-engineered skin.Несмотря на достижения современной хирургии в лечении повреждений кожных покровов, актуальным остается поиск новых методов для более быстрого и эффективного заживления ран. Тканевая инженерия, несомненно, представляет интерес для разработки таких технологий. Цель данной работы состояла в определении оптимального протокола получения децеллюляризированного дермального матрикса для последующей разработки тканеинженерной кожи. Материалы и методы. Экспериментальным животным был 1 поросенок породы Ландрас. После предварительной обработки кожи дерматомом забирали образцы толщиной 0,3 см. В работе рассматривалось 2 протокола децеллюляризации: протокол № 1 на основе применения тритон Х100 и дезоксихолата, протокол № 2 только на основе дезоксихолата. Всего циклов обработки по 2 протоколам было 5. Ацеллюлярные матриксы после обработки были исследованы следующим образом: гистологический анализ, количественное определение содержания ДНК. Далее была проведена статическая рецеллюляризация матриксов фибробластами дермы свиньи. После чего матриксы были исследованы на цитотоксичность с помощью ХТТ-теста и теста на дифференциальное окрашивание живых и погибших клеток. Результаты. Проведенный сравнительный анализ двух протоколов децеллюляризации дермы свиной кожи показал, что оба протокола эффективно удаляют клетки и ядерный материал, при этом сохраняется архитектоника межклеточного вещества неповрежденной, так как не происходит разрушения волокнистых структур. Но при оценке биосовместимости матриксов на основе анализа жизнеспособности клеток по данным ХТТ-теста и адгезии клеток к матриксу преимущества демонстрирует матрикс, обработанный по протоколу № 1. Заключение. В настоящем исследовании был отмечен протокол децеллюляризации на основе тритон Х100 и дезоксихолата. Полученные результаты являются первым этапом для дальнейшей разработки тканеинженерной кожи

Ocrelizumab versus Interferon Beta-1a in Relapsing Multiple Sclerosis

Supported by F. Hoffmann–La Roche