Preliminary assessment of various additives on the specific reactivity of anti- rHBsAg monoclonal antibodies

Background: Antibodies have a wide application in diagnosis and treatment. In order to maintain optimal stability of various functional parts of antibodies such as antigen binding sites, several approaches have been suggested. Using additives such as polysaccharides and polyols is one of the main methods in protecting antibodies against aggregation or degradation in the formulation. The aim of this study was to evaluate the protective effect of various additives on the specific reactivity of monoclonal antibodies (mAbs) against recombinant HBsAg (rHBsAg) epitopes. Methods: To estimate the protective effect of different additives on the stability of antibody against conformational epitopes (S3 antibody) and linear epitopes (S7 and S11 antibodies) of rHBsAg, heat shock at 37°C was performed in liquid and solid phases. Environmental factors were considered to be constant. The specific reactivity of antibodies was evaluated using ELISA method. The data were analyzed using SPSS software by Mann-Whitney nonparametric test with the confidence interval of 95%. Results: Our results showed that 0.25 M sucrose, 0.04 M trehalose and 0.5% BSA had the most protective effect on maintaining the reactivity of mAbs (S3) against conformational epitopes of rHBsAg. Results obtained from S7 and S11 mAbs against linear characteristics showed minor differences. The most efficient protective additives were 0.04 M trehalose and 1 M sucrose. Conclusion: Nowadays, application of appropriate additives is important for increasing the stability of antibodies. It was concluded that sucrose, trehalose and BSA have considerable effects on the specific reactivity of anti rHBsAg mAbs during long storage. © 2015, Avicenna Journal of Medical Biotechnology. All rights reserved

Effectiveness Of Council Operations And Particiaption Of Inhabitants In Council Activities In Rural Mirabad, Isfahan Province Of Iran

This paper discusses council operation, public expectations of people from councils, public participation and coordination of people with councils also factors which affect this kind of participation. Public participation and coordination has been studied in Mirabad village of Tiran and Krun, 2 cities of Esfahan from Iran. This village is located 43 kilometers far from the west of Isfahan, Iran. Its population is more than 70 thousand persons and its climate is cold. The major occupation of its people is agriculture and animal husbandry in this region. The village\'s council members belong to the average class of community their educational range varies between having elementary school certificate to having licentiate\'s degree. Data gathered via questionnaire, interview and observation. 30 questionnaires were distributed among Mirabad\'s people and filled out. Most of the time, along with filling out the questionnaire, an interview was also accomplished and some observation were also made, The results show that council operations such as communicating with people, unanimity, sympathy and getting public trust were effective in receiving public participation and cooperation and also help the councils to do their tasks well. People have good participation in many fields like security maintenance, providing environmental hygiene and maintenance of public installations. The council\'s members also have good participation in agricultural affairs, providing security and hygiene and pursuing constructional plans. Keywords: participation, participation factors, councils operationJournal of Agriculture and Social Research Vol. 8 (1) 2008: pp. 106-11

Determination of the best canopy gap area on the basis of soil characteristics using the Analytical Hierarchy Process (AHP)

The assessment of canopy gap areas on the basis of soil characteristics in forest ecosystems could be one of benefit points for management of forests. This research was conducted in 20 ha areas of Experimental Forest Station of Tarbiat Modares University that is located in a temperate forest of Mazandaran province in the north of Iran. Twenty one canopy gaps with different areas were found in studied areas and classified as small (85.12 m2), medium (325.21 m2), large (512.11 m2) and very large (723.85 m 2) gaps. These areas classes of canopy gaps were assessed with respect to nine criteria (soil pH, carbon to nitrogen ratio, cation exchange capacity, phosphorus, potassium, calcium, nitrogen mineralization, microbial respiration and earthworm's biomass). Soil samples (0-45 cm depth from the gap center and edge positions) were measured in the laboratory. The Analytical Hierarchy Process (AHP) was used for assessment of canopy gap areas. This method is widely used the Multiple Criteria Decision Support (MCDS) method and perhaps the most popular in many fields, including natural resource management, especially in forest sciences. Results of AHP indicate that the maximum of local priority belongs to small areas of canopy gaps when considering all soil characteristics. However, medium, large and very large canopy gap areas have priorities, respectively. The calculated overall priority showed that with respect to considered criterias, small and medium gap areas have higher, more ideal condition in comparison to large and very large areas. AHP results emphasise that considering soil characteristics canopy gap areas should be less than 400 m2 in Hyrcanian forests of Iran. Also, AHP can be introduced as an effective instrument in decision-making processes for investment planning and prioritization in compliance with environmental regulations

Evaluation of the spatial arrangement of Purkinje cells in ataxic rat’s cerebellum after Sertoli cells transplantation

Background: Purkinje cells (PCs) pathology is important in cerebellar disorders like ataxia. The spatial arrangement of PCs after different treatments has not been studied extensively. Immunohistochemistry (IHC) analysis of cerebellum can give a proper tool for explaining the pathophysiology of PCs in ataxia. Here we stereologically analysed the 3-dimensional spatial arrangement of PCs in the cerebellum of rats after ataxia induction with 3-acetylpyridine (3-AP). Materials and methods: Ataxia was induced in rats by intraperitoneal injection of 3-AP (65 mg/kg). Spatial arrangement of PCs for differences in ataxic rats with (3-AP-SC) or without (3-AP) Sertoli cells (SCs) transplantation was evaluated using second-order stereology. The IHC method by using antibodies to anti-calbindin in the cerebellum was applied. Results: Our results showed that a random arrangement is at larger distances between PCs in 3-AP and 3-Ap-SC groups. Therefore the PCs are not normally arranged after 3-AP and SCs transplantation stored the spatial arrangements of the cells after ataxia induction in rats. IHC analyse shows that number of PCs was significantly improved after the SC transplantation. Conclusions: Segregation of PCs can be observed at some areas in the ataxic rats’ cerebellum. However, the spatial arrangement of PCs was unchanged in SCs transplanted rats. (Folia Morphol 2018; 77, 2: 194–200

Introducing a sustainable soil fertility system for chickpea (Cicer arietinum L.)

In order to introduce a sustainable soil fertility system for chickpea, field experiments were carried out in 2007 and 2008 growing seasons. Experiments were arranged in split-split plot arrangements with three replications. Main plots consisted of (G1): establishing a mixed vegetation of vetch and barley, (G2): without green manure. Also, five strategies for obtaining the base fertilizer requirement including (N1): farm manure; (N2): compost; (N3): triple super phosphate; (N4): farm manure + compost and (N5): farm manure + compost + triple super phosphate. Four levels of biofertilizers consisted of (B1): Phosphate solubilizing bacteria; (B2): Trichoderma harzianum; (B3): Phosphate solubilizing bacteria + T. harzianum; and (B4): without biofertilizers were arranged in sub-sub plots. Results showed that green manure increased pod number and number of fertile pods per plant. Integrating biofertilizers (B3) and green manure (G1) produced the highest pod number per plant and grain yield. The highest amounts of yield and yield components were obtained in G1N5 treatment. Comparison of 3-way interactions showed that G1N5B3 was the best treatment. Significant increase of N, P, K, Fe and Mg content in leaves and grains emphasized on superiority of mentioned treatment because each one of these nutrients has an approved role in chlorophyll synthesis and photosynthesis ability of the crop. The N5 treatment in addition to having the highest yield had the best grain quality due to high protein, starch and total sugar contents, low crude fiber and reduced cooking time.Key words: Biofertilizer, chickpea, compost, farm manure, soil fertility system

Effects of biofertilizers on grain yield and protein content of two soybean (Glycine max L.) cultivars

Nutrient management is one of the most important factors in successful cultivation of plants. Biofertilizers can affect the quality and quantity of crop. In order to study the effects of biofertilizers on grain yield and protein content of two soybean (Glycine max L.) cultivars, an experiment was conducted using a factorial arrangement based on randomized complete block design with four replications, at the Mahidasht Research Station of Kermanshah in 2010. The factors were soybean cultivar (Williams and Line no. 17) and fertilizer application (b1= N + P, b2= Bradyrhizobium japonicum + P, b3= N + Bacillus and Pseudomonas + 50% of P, b4= B. japonicum + Bacillus and Pseudomonas + 50% of P, b5= B. japonicum + 50% of N + Bacillus and Pseudomonas + 50% of P). Results show that Line no. 17 with 2911.2 kg/ha had higher seed yield than Williams with 2711 kg/ha. Also, fertilizer levels of b3 with 3058.2 and b2 with 2643.8 kg/ha produced the highest and the lowest seed yield, respectively. Plants treated with fertilizer levels of b1, b2 and b5 in comparison with other fertilizer levels significantly produced lower thousand seed weight. In Line no. 17 fertilizer level of b3 with 2.88 produced the highest seed per pod. Results show that fertilizer levels had a significant effect on the number of pod per plant and treatments containing biological fertilizers in terms of the number of pods per plant were equal or superior to chemical fertilizer. It was also observed that fertilizer levels of b1, b3 and b5, produced the highest protein percentage. It therefore seems that biofertilizers can be considered as a replacement for part of chemical fertilizers in soybean production.Key words: Bacillus, Bradyrhizobium japonicum, phosphate solubilizing, protein, Pseudomonas

Pollen-pistil compatibility relationships in some Iranian almond (Prunus dulcis, Batch) genotypes as revealed by PCR analysis

The identification of pollen-pistil compatibility relationships among almond cultivars and genotypes is very important for breeders and growers. In the present study, PCR based technique was used to identify S-alleles in 10 late blooming almond genotypes. In total, 19 alleles were amplified by five primer pairs in the studied genotypes. The size of bands ranged between 480 - 2000 bp. Seven S-alleles were amplified using AS1II/AMYC5R primer pair, whereas each of the Alsc11/AMYC5R, Pru-C2/Pru-C4R, Pru-C2/Pru-C5R and Pru-C2/Pru-C6R primer pairs amplified nine different S-alleles. Based on S-allele patterns, all of the studied genotypes were identified as self-incompatible. However, some of the genotypes had only one similar S-allele, all of the genotypes could be used in establishment of commercial orchards based on their blooming times

Reconstruction of modified gravity with ghost dark energy models

In this work, we reconstruct the $f(R)$ modified gravity for different ghost and generalized ghost dark energy models in FRW flat universe, which describe the accelerated expansion of the universe. The equation of state of reconstructed $f(R)$ - gravity has been calculated. We show that the corresponding $f(R)$ gravity of ghost dark energy model can behave like phantom or quintessence. We also show that the equation of state of reconstructed $f(R)$ gravity for generalized ghost model can transit from quintessence regime to the phantom regime as indicated by recent observations.Comment: 13 pages, some references and one author are added. Accepted for publication by MPL

Magnetic Interactions of Neighbouring Stator Sets in Multi DOF Local Electromagnetic Actuation for Robotic Abdominal Surgery

This paper aims to characterise the magnetic interaction in neighbouring sets of local electromagnetic actuation (LEMA) actuators in a robotic platform for abdominal surgery. The analysis looks into the affect of the magnetic fields contributed by a stator-rotor set (the actuation unit) located adjacent to the rotor of interest. Each rotor drives one of the degree-of-freedoms (DOFs) on a surgical robotic device. In this study, a two-DOF setup is used for the magnetic interaction analysis, which can be expanded to general case n-DOF setup with the Principle of Superposition of magnetic fields from multiple sources. The magnetic model is then used to compute the dynamics of the system, which involves the equation of motion of the rotors and associated robotic mechanism it drives, and the actuator (electrical) model that takes into account the back EMF generated by the permanent magnet rotors. The magnetic field effect of the neighbouring set onto the rotor is observed by obtaining the speed response of the rotor through simulation so that the dynamic model can be validated against the experimental results. The outcomes are useful for the design specification of the LEMA system configuration, involving the feasible / pragmatic distance between the stator sets such that the interference is minimised, and for the design of the necessary control strategy