8 research outputs found
Clinical application of induced sputum in children with newly diagnosed asthma: cellular and immunologic characteristics
Authors investigate cells and immunologic factors of induced sputum in children with newly diagnosed asthma and healthy children without atopy. The aim of the study was to find out the differences of cellular and immunologic profiles of induced sputum in children with newly diagnosed asthma. 35 children aged 1,5-5 years old (Me = 3,5 years) were include in this study: 18 children with newly diagnosed asthma and 17 children (control group) without allergic diseases, which had no respiratory symptoms during last month. Sputum induction carried out according to our modification of protocol developed by Pin et al. The levels of IgE, slgA, lgG4, IL-1Ξ², IL-4, IL-8, IL-13, TNFΞ±, INFΞ³, N03, NOX and cells percentage (macrophages, neutrophils, eosinophils, lymphocytes) were evaluated in sputum. Results. The percentage of eosinophils was significantly higher and the percentage of macrophages was significantly lower in induced sputum of children with newly diagnosed asthma. The levels of proinflammatory factors (IL-1Ξ², IL-4, IL-8, IL-13, TNFΞ±), immunoglobulins, which participate in allergic inflammation (IgE, slgA, lgG4) and stable metabolites of NO (NO3, NOX) in sputum were also significantly higher in children with newly diagnosed asthma.ΠΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΡΡ ΠΏΠΎΠ΄ΡΡΠ΅Ρ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² Π² ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΠ΅ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π²ΠΏΠ΅ΡΡΡΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ ΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄Π΅ΡΠ΅ΠΉ Π±Π΅Π· Π°ΡΠΎΠΏΠΈΠΈ. Π¦Π΅Π»ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΎ Π²ΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΡΠΈΡΠΎΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΡ Ρ Π΄Π΅ΡΠ΅ΠΉ ΡΠ°Π½Π½Π΅Π³ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ° Ρ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΡΠΈΠ½ΡΠ»ΠΈ ΡΡΠ°ΡΡΠΈΠ΅ 35 Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ 1,5-5 Π»Π΅Ρ (ΡΡΠ΅Π΄Π½ΠΈΠΉ Π²ΠΎΠ·ΡΠ°ΡΡ 3,5Π³.): 18 Π΄Π΅ΡΠ΅ΠΉ Ρ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ ΠΈ 17 ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄Π΅ΡΠ΅ΠΉ Π±Π΅Π· Π°ΡΠΎΠΏΠΈΠΈ - ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ Π³ΡΡΠΏΠΏΠ°. Π£ Π²ΡΠ΅Ρ
Π΄Π΅ΡΠ΅ΠΉ Π² ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²ΡΡΡΠ΅Π³ΠΎ ΠΌΠ΅ΡΡΡΠ° Π½Π΅ Π±ΡΠ»ΠΎ Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΡΠΏΠΈΠ·ΠΎΠ΄ΠΎΠ² ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ. ΠΠ½Π΄ΡΠΊΡΠΈΡ ΠΌΠΎΠΊΡΠΎΡΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡ ΠΏΠΎ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΌΡ Π½Π°ΠΌΠΈ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Ρ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π³ΠΈΠΏΠ΅ΡΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°ΡΡΠ²ΠΎΡΠ° Ρ
Π»ΠΎΡΠΈΠ΄Π° Π½Π°ΡΡΠΈΡ. ΠΡΠΎΡΠΎΡΠΈΠΏΠΎΠΌ ΡΠ²ΠΈΠ»ΡΡ ΠΌΠ΅ΡΠΎΠ΄, ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΠΉ Pin et al., ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π»ΡΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠΉ ΡΠΎΡΡΠ°Π² (%) (ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΈ, Π½Π΅ΠΉΡΡΠΎΡΠΈΠ»Ρ, ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»Ρ, Π»ΠΈΠΌΡΠΎΡΠΈΡΡ) ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΏΡΠΎΡΠΈΠ»Ρ (IgE, slgA, lgG4, IL-1Ξ², IL-4, IL-8, IL-13. TNFΞ±, INFΞ³, NO3, NOX) ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΡ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π£ Π΄Π΅ΡΠ΅ΠΉ Ρ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ Π² ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΠ΅ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»ΠΎΠ² (%) Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π²ΡΡΠ΅, Π° ΡΡΠΎΠ²Π΅Π½Ρ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² (%) Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π½ΠΈΠΆΠ΅ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΡΠΎ Π·Π΄ΠΎΡΠΎΠ²ΡΠΌΠΈ Π΄Π΅ΡΡΠΌΠΈ Π±Π΅Π· Π°ΡΠΎΠΏΠΈΠΈ. Π’Π°ΠΊ ΠΆΠ΅ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ Π²ΡΡΠ²Π»Π΅Π½Ρ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΠΈΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² (Π€ΠΠΠ°, ΠΠ 4, IL-1B, ΠΠ8, ΠΠ 13), ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½ΠΎΠ² (IgE, lgG4) ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ
Π² Π°Π»Π»Π΅ΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠΈ Π±ΡΠΎΠ½Ρ
ΠΎΠ² ΠΏΡΠΈ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠ΅ ΠΈ ΠΊΠΎΠ½Π΅ΡΠ½ΡΡ
ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΡΡ
ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠΎΠ² ΠΎΠΊΡΠΈΠ΄Π° Π°Π·ΠΎΡΠ° (NO3, NOX) ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄Π΅ΡΠ΅ΠΉ Π±Π΅Π· Π°ΡΠΎΠΏΠΈΠΈ
ΠΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π°Π»ΡΡΠ°-2b ΠΏΡΠΈ Π±ΡΠΎΠ½Ρ ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠ΅ Ρ Π΄Π΅ΡΠ΅ΠΉ
A prospective cohort study was conducted to assess the effect of a recombinant interferon alpha-2b prophylactic course on cyto-immunological parameters of induced sputum in children with mild asthma, the main trigger of exacerbation of which was respiratory viral infections.We examined 40 children aged 1 to 7 years with (mean age β 4.8 Β± 0.2 years), half of whom received a course of recombinant interferon alpha-2b. The authors detected respiratory viruses in scrapings from throat and nose (PCR method) and evaluated the different cell counts, also the levels of interleukin-10, tumor necrosis factor alpha, interferon alpha and immunoglobulin E on the 1st and 30th day of the study (ELISA method) were determined in sputum.The number of asthma exacerbations, triggered by respiratory viral infection, decreased in 4.5 times against. In children who received recombinant interferon alpha-2b the count of sputum eosinophils and the number of children with sputum eosinophilia (β₯3 %) reduced. After two months the level of nasal eosinophils was 30% lower than the start level. The level of IgE in induced sputum decreased by 35%, but the level of IL-10 was increased by 1.5 times. 75% of parents whose children received recombinant interferon alpha-2b, note positive effect of this drug.There were no adverse events to receive the drug in any case. Thus, the use of recombinant interferon alpha-2b in children with virus-induced asthma to reduce episodes of exacerbations associated with acute respiratory diseases can be recommended.ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΏΡΠΎΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ΅ ΠΊΠΎΠ³ΠΎΡΡΠ½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ ΡΠ΅Π»ΡΡ ΠΎΡΠ΅Π½ΠΊΠΈ Π²Π»ΠΈΡΠ½ΠΈΡ ΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΡΡΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π°Π»ΡΡΠ°-2b (ΡΡΠΏΠΏΠΎΠ·ΠΈΡΠΎΡΠΈΠΈ ΠΈ Π³Π΅Π»Ρ) Π½Π° ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΈ ΡΠΈΡΠΎΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΡ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π»Π΅Π³ΠΊΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ, ΠΎΡΠ½ΠΎΠ²Π½ΡΠΌ ΡΡΠΈΠ³Π³Π΅ΡΠΎΠΌ ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΡ ΠΊΠΎΡΠΎΡΠΎΠΉ ΡΠ²Π»ΡΠ»ΠΈΡΡ ΠΎΡΡΡΡΠ΅ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠ΅ Π²ΠΈΡΡΡΠ½ΡΠ΅ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ.Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π±ΡΠ»ΠΎ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΎ 40 Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 1 Π΄ΠΎ 7 Π»Π΅Ρ (ΡΡΠ΅Π΄Π½ΠΈΠΉ Π²ΠΎΠ·ΡΠ°ΡΡ β 4,8 Β± 0,2 Π»Π΅Ρ), ΠΏΠΎΠ»ΠΎΠ²ΠΈΠ½Π° ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ
ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ ΠΊΡΡΡ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π°Π»ΡΡΠ°-2b ΠΏΠΎ ΡΡ
Π΅ΠΌΠ΅ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 29 Π΄Π½Π΅ΠΉ. ΠΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΎΡΡ ΡΠΈΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π½Π°Π·Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΠΊΡΠ΅ΡΠ° ΠΈ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΡ, ΠΌΠ°Π·ΠΊΠΎΠ² ΠΈΠ· Π·Π΅Π²Π° ΠΈ Π½ΠΎΡΠ° Π½Π° ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠ΅ Π²ΠΈΡΡΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ¦Π , ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ€Π Π² ΠΌΠΎΠΊΡΠΎΡΠ΅ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΡΠΎΠ²Π½ΠΈ ΠΈΠ½ΡΠ΅ΡΠ»Π΅ΠΉΠΊΠΈΠ½Π°-10, ΡΠ°ΠΊΡΠΎΡΠ° Π½Π΅ΠΊΡΠΎΠ·Π° ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π°Π»ΡΡΠ°, ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π°Π»ΡΡΠ° ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½Π° Π, Π½Π° 1-ΠΉ ΠΈ 30-ΠΉ Π΄Π΅Π½Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ.ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΠΉ Π°ΡΡΠΌΡ Π½Π° ΡΠΎΠ½Π΅ ΠΎΡΡΡΡΡ
ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ Π² 4,5 ΡΠ°Π·Π°. Π£ Π΄Π΅ΡΠ΅ΠΉ, ΠΏΠΎΠ»ΡΡΠΈΠ²ΡΠΈΡ
ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΠΉ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½ Π°Π»ΡΡΠ°-2b, ΠΎΡΠΌΠ΅ΡΠ΅Π½ΠΎ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΠΈ Π°Π»Π»Π΅ΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ Π½Π° ΡΡΠΎΠ²Π½Π΅ ΡΠ»ΠΈΠ·ΠΈΡΡΡΡ
ΠΎΠ±ΠΎΠ»ΠΎΡΠ΅ΠΊ Π½ΠΎΡΠ° ΠΈ Π±ΡΠΎΠ½Ρ
ΠΎΠ². ΠΠ½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠ½ΠΈΠ·ΠΈΠ»ΠΎΡΡ ΠΊΠ°ΠΊ ΡΡΠ΅Π΄Π½Π΅Π΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»ΠΎΠ² Π² ΠΌΠΎΠΊΡΠΎΡΠ΅ β Π² 7,6 ΡΠ°Π·, ΡΠ°ΠΊ ΠΈ ΡΠΈΡΠ»ΠΎ Π΄Π΅ΡΠ΅ΠΉ Ρ ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»ΠΈΠ΅ΠΉ ΠΌΠΎΠΊΡΠΎΡΡ (β₯ 3%). Π£ΡΠΎΠ²Π΅Π½Ρ ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»ΠΎΠ² Π½Π°Π·Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΠΊΡΠ΅ΡΠ° ΡΠ΅ΡΠ΅Π· 2 ΠΌΠ΅ΡΡΡΠ° ΠΎΡ Π½Π°ΡΠ°Π»Π° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ» Π½ΠΈΠΆΠ΅ ΠΏΠ΅ΡΠ²ΠΎΠ½Π°ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π½Π° 30%. ΠΠ°ΡΠ°Π»Π»Π΅Π»ΡΠ½ΠΎ ΠΏΡΠΎΠΈΠ·ΠΎΡΠ»ΠΎ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ IgE Π² ΠΌΠΎΠΊΡΠΎΡΠ΅ Π½Π° 35% ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ IL-10 Π² 1,5 ΡΠ°Π·Π°. Π Π³ΡΡΠΏΠΏΠ΅ Π΄Π΅ΡΠ΅ΠΉ, ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΠΉ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½ Π°Π»ΡΡΠ°-2b, 75% ΡΠΎΠ΄ΠΈΡΠ΅Π»Π΅ΠΉ ΠΏΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎ Π²ΡΡΠΊΠ°Π·Π°Π»ΠΈΡΡ ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΠΎΠΌ ΠΊΡΡΡΠ΅. ΠΠ΅ Π±ΡΠ»ΠΎ ΠΎΡΠΌΠ΅ΡΠ΅Π½ΠΎ Π½Π΅ΠΆΠ΅Π»Π°ΡΠ΅Π»ΡΠ½ΡΡ
ΡΠ²Π»Π΅Π½ΠΈΠΉ Π½Π° ΠΏΡΠΈΠ΅ΠΌ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° Π½ΠΈ Π² ΠΎΠ΄Π½ΠΎΠΌ ΡΠ»ΡΡΠ°Π΅. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄ΠΎΠ²Π°ΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΊΡΡΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π°Π»ΡΡΠ°-2b Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π²ΠΈΡΡΡ-ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ Π΄Π»Ρ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΡ ΡΠΏΠΈΠ·ΠΎΠ΄ΠΎΠ² ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΠΉ, ΡΠ²ΡΠ·Π°Π½Π½ΡΡ
Ρ ΠΎΡΡΡΡΠΌΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΠΌΠΈ
Application of NMR for quantification of magnetic nanoparticles and development of paper-based assay
H1 NMR relaxometry is a method that is extremely sensitive to the presence of magnetic nanoparticles, which significantly affect the transverse relaxation time of the water proton. Accordingly, the use of magnetic nanoparticles as labels allows detection of even extremely small amounts of the test substance. This paper analyzes the prospects for applying the method of solid-phase NMR-relaxometric determination of biologically active molecules. The nitrocellulose membranes are chosen as a solid phase and nanoparticles based on iron core with a carbon shell are used as magnetic labels. The possibility of detecting small concentrations of magnetic particles in porous medium is demonstrated. Finally, the ability to detect extremely low concentrations of an analyte, in this case, streptavidin protein (0.5 ng/ml to 100 ng/ml), which is actively used in various fields of biology and medicine, is demonstrated. Β© Published under licence by IOP Publishing Ltd.Russian Science Foundation,Β RSF: 17-15-01116The work was carried out within the Russian Science Foundation project 17-15-01116. equipment of the Ural Center for Shared Use Modern nanotechnology UrFU was used
Π’he prophylactic efficacy of a recombinant interferon alfa-2b drug in children with bronchial asthma
A prospective cohort study was conducted to assess the effect of a recombinant interferon alpha-2b prophylactic course on cyto-immunological parameters of induced sputum in children with mild asthma, the main trigger of exacerbation of which was respiratory viral infections.We examined 40 children aged 1 to 7 years with (mean age β 4.8 Β± 0.2 years), half of whom received a course of recombinant interferon alpha-2b. The authors detected respiratory viruses in scrapings from throat and nose (PCR method) and evaluated the different cell counts, also the levels of interleukin-10, tumor necrosis factor alpha, interferon alpha and immunoglobulin E on the 1st and 30th day of the study (ELISA method) were determined in sputum.The number of asthma exacerbations, triggered by respiratory viral infection, decreased in 4.5 times against. In children who received recombinant interferon alpha-2b the count of sputum eosinophils and the number of children with sputum eosinophilia (β₯3 %) reduced. After two months the level of nasal eosinophils was 30% lower than the start level. The level of IgE in induced sputum decreased by 35%, but the level of IL-10 was increased by 1.5 times. 75% of parents whose children received recombinant interferon alpha-2b, note positive effect of this drug.There were no adverse events to receive the drug in any case. Thus, the use of recombinant interferon alpha-2b in children with virus-induced asthma to reduce episodes of exacerbations associated with acute respiratory diseases can be recommended
ΠΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π°Π»ΡΡΠ°-2b ΠΏΡΠΈ Π±ΡΠΎΠ½Ρ ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠ΅ Ρ Π΄Π΅ΡΠ΅ΠΉ
A prospective cohort study was conducted to assess the effect of a recombinant interferon alpha-2b prophylactic course on cyto-immunological parameters of induced sputum in children with mild asthma, the main trigger of exacerbation of which was respiratory viral infections.We examined 40 children aged 1 to 7 years with (mean age β 4.8 Β± 0.2 years), half of whom received a course of recombinant interferon alpha-2b. The authors detected respiratory viruses in scrapings from throat and nose (PCR method) and evaluated the different cell counts, also the levels of interleukin-10, tumor necrosis factor alpha, interferon alpha and immunoglobulin E on the 1st and 30th day of the study (ELISA method) were determined in sputum.The number of asthma exacerbations, triggered by respiratory viral infection, decreased in 4.5 times against. In children who received recombinant interferon alpha-2b the count of sputum eosinophils and the number of children with sputum eosinophilia (β₯3 %) reduced. After two months the level of nasal eosinophils was 30% lower than the start level. The level of IgE in induced sputum decreased by 35%, but the level of IL-10 was increased by 1.5 times. 75% of parents whose children received recombinant interferon alpha-2b, note positive effect of this drug.There were no adverse events to receive the drug in any case. Thus, the use of recombinant interferon alpha-2b in children with virus-induced asthma to reduce episodes of exacerbations associated with acute respiratory diseases can be recommended.ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΏΡΠΎΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ΅ ΠΊΠΎΠ³ΠΎΡΡΠ½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ ΡΠ΅Π»ΡΡ ΠΎΡΠ΅Π½ΠΊΠΈ Π²Π»ΠΈΡΠ½ΠΈΡ ΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΡΡΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π°Π»ΡΡΠ°-2b (ΡΡΠΏΠΏΠΎΠ·ΠΈΡΠΎΡΠΈΠΈ ΠΈ Π³Π΅Π»Ρ) Π½Π° ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΈ ΡΠΈΡΠΎΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΡ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π»Π΅Π³ΠΊΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ, ΠΎΡΠ½ΠΎΠ²Π½ΡΠΌ ΡΡΠΈΠ³Π³Π΅ΡΠΎΠΌ ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΡ ΠΊΠΎΡΠΎΡΠΎΠΉ ΡΠ²Π»ΡΠ»ΠΈΡΡ ΠΎΡΡΡΡΠ΅ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠ΅ Π²ΠΈΡΡΡΠ½ΡΠ΅ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ.Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π±ΡΠ»ΠΎ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΎ 40 Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 1 Π΄ΠΎ 7 Π»Π΅Ρ (ΡΡΠ΅Π΄Π½ΠΈΠΉ Π²ΠΎΠ·ΡΠ°ΡΡ β 4,8 Β± 0,2 Π»Π΅Ρ), ΠΏΠΎΠ»ΠΎΠ²ΠΈΠ½Π° ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ
ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ ΠΊΡΡΡ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π°Π»ΡΡΠ°-2b ΠΏΠΎ ΡΡ
Π΅ΠΌΠ΅ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 29 Π΄Π½Π΅ΠΉ. ΠΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΎΡΡ ΡΠΈΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π½Π°Π·Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΠΊΡΠ΅ΡΠ° ΠΈ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΡ, ΠΌΠ°Π·ΠΊΠΎΠ² ΠΈΠ· Π·Π΅Π²Π° ΠΈ Π½ΠΎΡΠ° Π½Π° ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠ΅ Π²ΠΈΡΡΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ¦Π , ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ€Π Π² ΠΌΠΎΠΊΡΠΎΡΠ΅ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΡΠΎΠ²Π½ΠΈ ΠΈΠ½ΡΠ΅ΡΠ»Π΅ΠΉΠΊΠΈΠ½Π°-10, ΡΠ°ΠΊΡΠΎΡΠ° Π½Π΅ΠΊΡΠΎΠ·Π° ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π°Π»ΡΡΠ°, ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π°Π»ΡΡΠ° ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½Π° Π, Π½Π° 1-ΠΉ ΠΈ 30-ΠΉ Π΄Π΅Π½Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ.ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΠΉ Π°ΡΡΠΌΡ Π½Π° ΡΠΎΠ½Π΅ ΠΎΡΡΡΡΡ
ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ Π² 4,5 ΡΠ°Π·Π°. Π£ Π΄Π΅ΡΠ΅ΠΉ, ΠΏΠΎΠ»ΡΡΠΈΠ²ΡΠΈΡ
ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΠΉ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½ Π°Π»ΡΡΠ°-2b, ΠΎΡΠΌΠ΅ΡΠ΅Π½ΠΎ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΠΈ Π°Π»Π»Π΅ΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ Π½Π° ΡΡΠΎΠ²Π½Π΅ ΡΠ»ΠΈΠ·ΠΈΡΡΡΡ
ΠΎΠ±ΠΎΠ»ΠΎΡΠ΅ΠΊ Π½ΠΎΡΠ° ΠΈ Π±ΡΠΎΠ½Ρ
ΠΎΠ². ΠΠ½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠ½ΠΈΠ·ΠΈΠ»ΠΎΡΡ ΠΊΠ°ΠΊ ΡΡΠ΅Π΄Π½Π΅Π΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»ΠΎΠ² Π² ΠΌΠΎΠΊΡΠΎΡΠ΅ β Π² 7,6 ΡΠ°Π·, ΡΠ°ΠΊ ΠΈ ΡΠΈΡΠ»ΠΎ Π΄Π΅ΡΠ΅ΠΉ Ρ ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»ΠΈΠ΅ΠΉ ΠΌΠΎΠΊΡΠΎΡΡ (β₯ 3%). Π£ΡΠΎΠ²Π΅Π½Ρ ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»ΠΎΠ² Π½Π°Π·Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΠΊΡΠ΅ΡΠ° ΡΠ΅ΡΠ΅Π· 2 ΠΌΠ΅ΡΡΡΠ° ΠΎΡ Π½Π°ΡΠ°Π»Π° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ» Π½ΠΈΠΆΠ΅ ΠΏΠ΅ΡΠ²ΠΎΠ½Π°ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π½Π° 30%. ΠΠ°ΡΠ°Π»Π»Π΅Π»ΡΠ½ΠΎ ΠΏΡΠΎΠΈΠ·ΠΎΡΠ»ΠΎ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ IgE Π² ΠΌΠΎΠΊΡΠΎΡΠ΅ Π½Π° 35% ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ IL-10 Π² 1,5 ΡΠ°Π·Π°. Π Π³ΡΡΠΏΠΏΠ΅ Π΄Π΅ΡΠ΅ΠΉ, ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΠΉ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½ Π°Π»ΡΡΠ°-2b, 75% ΡΠΎΠ΄ΠΈΡΠ΅Π»Π΅ΠΉ ΠΏΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎ Π²ΡΡΠΊΠ°Π·Π°Π»ΠΈΡΡ ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΠΎΠΌ ΠΊΡΡΡΠ΅. ΠΠ΅ Π±ΡΠ»ΠΎ ΠΎΡΠΌΠ΅ΡΠ΅Π½ΠΎ Π½Π΅ΠΆΠ΅Π»Π°ΡΠ΅Π»ΡΠ½ΡΡ
ΡΠ²Π»Π΅Π½ΠΈΠΉ Π½Π° ΠΏΡΠΈΠ΅ΠΌ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° Π½ΠΈ Π² ΠΎΠ΄Π½ΠΎΠΌ ΡΠ»ΡΡΠ°Π΅. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄ΠΎΠ²Π°ΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΊΡΡΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π°Π»ΡΡΠ°-2b Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π²ΠΈΡΡΡ-ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ Π΄Π»Ρ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΡ ΡΠΏΠΈΠ·ΠΎΠ΄ΠΎΠ² ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΠΉ, ΡΠ²ΡΠ·Π°Π½Π½ΡΡ
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Environmental Changes of the Aral Sea (Central Asia) in the Holocene: Major Trends
From the 20th International Radiocarbon Conference held in Kona, Hawaii, USA, May 31-June 3, 2009.Changes of the Aral Sea level have been observed in 3 sediment boreholes, 2 outcrops, and associated archaeological sites. The obtained results are supported by 25 radiocarbon dates. Major trends of lake-level changes have been reconstructed in some detail for the last 2000 yr, and additional data provide an outline of fluctuations throughout the Holocene. Several distinct changes are shown to precede the modern, human-induced regression of the Aral Sea. These include: 1) the latest maximum in the 16th-20th centuries AD (53 m asl); 2) a Medieval "Kerderi" minimum of the 12th-15th centuries AD (29 m asl); 3) the early Medieval maximum of the 4th-11th centuries AD (52 m asl); and 4) a near BC/AD lowstand, whose level is not well established. Since then, events are only inferred from sparse data. The studied cores contain several sandy layers representing the lowering of the lake level within the Holocene, including the buried shore-bar of ~4500 cal BP (38 m asl), and shallow-water sediments of ~5600 cal BP (44 m asl), 7200 cal BP (28 m asl), and 8000 cal BP (26.5 m asl).The Radiocarbon archives are made available by Radiocarbon and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform February 202