Isolation, sequence identification and tissue expression profile of a novel ribokinase gene (RBKS) from Chinese Banna mini-pig inbred line (BMI)

The complete expressed sequence tag (CDS) sequence of Banna mini-pig inbred line (BMI) ribokinase gene (RBKS) was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to accession number JF944892. Sequence analysis revealed that the BMI RBKS encodes a protein of 323 amino acids that has high homology with the ribokinase proteins of seven species: cattle (99%), horse (99%), orangutan (99%), human (89%), monkey (89%), rat (88%) and mouse (80%). The phylogenetic tree analysis revealed that the BMI RBKS gene has a closer genetic relationship with the RBKS genes of bovine and horse than with those of orangutan, human, monkey, rat and mouse. Analysis by RT-PCR showed that BMI RBKS gene was over-expressed in ovary and lung, moderately expressed in spleen, nerve fiber, large intestine and diencephalon, weakly expressed in heart, skin, muscle, small intestine, midbrain, kidney and fat, while almost silent in other five tissues. Four microRNA target sites were predicted in the CDS of BMI RBKS mRNA for further study of this gene in the future. The 3D structure of the RBKS by homology modeling was similar to that of human ribokinase (2fv7). Our experiment will establish a foundation for further insight into this swine gene.Key words: Banna mini-pig inbred line (BMI), pig, ribokinase gene (RBKS), tissue expression analysis, homology modelin

HST and LAMOST discover a dual active galactic nucleus in J0038+4128

We report the discovery of a kiloparsec-scale dual active galactic nucleus (AGN) in J0038+4128. From the Hubble Space Telescope (HST) Wide Field Planetary Camera (WFPC2) images, we find two optical nuclei with a projection separation of 4.7 kpc (3.44 arcsec). The southern component (J0038+4128S) is spectroscopically observed with the HST Goddard High Resolution Spectrograph in the UV range and is found to be a Seyfert 1 galaxy with a broad Ly alpha emission line. The northern component (J0038+4128N) is spectroscopically observed during the Large Sky Area Multi-Object Fibre Spectroscopic Telescope (also named the Guoshoujing Telescope) pilot survey in the optical range. The observed line ratios as well as the consistency of redshift of the nucleus emission lines and the host galaxy's absorption lines indicate that J0038+4128N is a Seyfert 2 galaxy with narrow lines only. These results thus confirm that J0038+4128 is a Seyfert 1-Seyfert 2 AGN pair. The HST WFPC2 F336W/U-band image of J0038+4128 also reveals for the first time for a dual AGN system two pairs of bi-symmetric arms, as are expected from the numerical simulations of such system. Being one of a few confirmed kiloparsec-scale dual AGNs exhibiting a clear morphological structure of the host galaxies, J0038+4128 provides an unique opportunity to study the co-evolution of the host galaxies and their central supermassive black holes undergoing a merging process.Comment: 6 pages, 4 figures, 2 tables, Accepted for publication in MNRAS Letter

Modelling of Photovoltaic Systems for Real-Time Hardware Simulation

The real-time simulation is a valid help to test electrical systems when a physical device is not available. This is significantly evident when used in hardware and software co-simulation environment, where it is possible to connect the emulator to a real subsystem to test or validate it. In this paper, a model of the photovoltaic system is presented that can be implemented within a hardware simulator to be able to interface it with a real circuit, the hardware simulator used is the National Instruments RIO system

Intelectin contributes to allergen-induced IL-25, IL-33, and TSLP expression and type 2 response in asthma and atopic dermatitis.

The epithelial and epidermal innate cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) have pivotal roles in the initiation of allergic inflammation in asthma and atopic dermatitis (AD). However, the mechanism by which the expression of these innate cytokines is regulated remains unclear. Intelectin (ITLN) is expressed in airway epithelial cells and promotes allergic airway inflammation. We hypothesized that ITLN is required for allergen-induced IL-25, IL-33, and TSLP expression. In two asthma models, Itln knockdown reduced allergen-induced increases in Il-25, Il-33, and Tslp and development of type 2 response, eosinophilic inflammation, mucus overproduction, and airway hyperresponsiveness. Itln knockdown also inhibited house dust mite (HDM)-induced early upregulation of Il-25, Il-33, and Tslp in a model solely inducing airway sensitization. Using human airway epithelial cells, we demonstrated that HDM-induced increases in ITLN led to phosphorylation of epidermal growth factor receptor and extracellular-signal regulated kinase, which were required for induction of IL-25, IL-33, and TSLP expression. In two AD models, Itln knockdown suppressed expression of Il-33, Tslp, and Th2 cytokines and eosinophilic inflammation. In humans, ITLN1 expression was significantly increased in asthmatic airways and in lesional skin of AD. We conclude that ITLN contributes to allergen-induced Il-25, Il-33, and Tslp expression in asthma and AD

Highly indistinguishable and strongly entangled photons from symmetric GaAs quantum dots

The development of scalable sources of non-classical light is fundamental to unlocking thetechnological potential of quantum photonics. Semiconductor quantum dots are emerging asnear-optimal sources of indistinguishable single photons. However, their performance assources of entangled-photon pairs are still modest compared to parametric down converters.Photons emitted from conventional Stranski–Krastanov InGaAs quantum dots have shownnon-optimal levels of entanglement and indistinguishability. For quantum networks, bothcriteria must be met simultaneously. Here, we show that this is possible with a system thathas received limited attention so far: GaAs quantum dots. They can emit triggered polar-ization-entangled photons with high purity (g(2)(0) = 0.002±0.002), high indistinguish-ability (0.93±0.07 for 2 ns pulse separation) and high entanglement fidelity(0.94±0.01). Our results show that GaAs might be the material of choice for quantum-dotentanglement sources in future quantum technologie

When and where do you want to hide? Recommendation of location privacy preferences with local differential privacy

In recent years, it has become easy to obtain location information quite precisely. However, the acquisition of such information has risks such as individual identification and leakage of sensitive information, so it is necessary to protect the privacy of location information. For this purpose, people should know their location privacy preferences, that is, whether or not he/she can release location information at each place and time. However, it is not easy for each user to make such decisions and it is troublesome to set the privacy preference at each time. Therefore, we propose a method to recommend location privacy preferences for decision making. Comparing to existing method, our method can improve the accuracy of recommendation by using matrix factorization and preserve privacy strictly by local differential privacy, whereas the existing method does not achieve formal privacy guarantee. In addition, we found the best granularity of a location privacy preference, that is, how to express the information in location privacy protection. To evaluate and verify the utility of our method, we have integrated two existing datasets to create a rich information in term of user number. From the results of the evaluation using this dataset, we confirmed that our method can predict location privacy preferences accurately and that it provides a suitable method to define the location privacy preference