1,163 research outputs found
“Molybdenum-based magnetic nanostructures/polymeric nanogels composites: Towards developing sustainable nanomaterials for virus detection"
“Molybdenum-based magnetic nanostructures/polymeric nanogels composites: Towards developing sustainable nanomaterials for virus detection"
Ultrasensitive Raman spectroscopy-based virus detection using glycan-coated plasmonic substrates
Hepatitis viral infections are the most common cause of hepatitis liver disease, which eventually leads to cancer and fibrosis if undetected early. Therefore, early detection would allow for preventive and therapeutic actions. Here, a surface-enhanced Raman spectroscopy (SERS)-based biosensor was developed using plasmonic molybdenum trioxide quantum dots (MoO3-QDs) as theSERS substrates. The nanostructured substrate of MoO3-QDs was functionalized with a proteoglycan (Syndecan-1) as a novel bioreceptor for target hepatitis E virus (HEV). The innovative bio-detection system achieved a detection limit of 1.05 fg/mL for tested HEV target (ORF2), indicating superb clinically relevant sensitivity and performance. The designed biosensing system incorporating a glycan motif as a bioreceptor instead of the conventional antibodies or aptamers,presents new insights for the ultrasensitive detection of HEV and other infectious viruses.<br/
Plasmonic Oleylamine-Capped Gold and Silver Nanoparticle-Assisted Synthesis of Luminescent Alloyed CdZnSeS Quantum Dots
We
report on a novel strategy to tune the structural and optical
properties of luminescent alloyed quantum dot (QD) nanocrystals using
plasmonic gold (Au) and silver (Ag) nanoparticles (NPs). Alloyed CdZnSeS
QDs were synthesized via the organometallic synthetic route with different
fabrication strategies that involve alternative utilization of blends
of organic surfactants, ligands, capping agents, and plasmonic oleylamine
(OLA)-functionalized AuNPs and AgNPs. Ligand exchange with thiol l-cysteine (l-cyst) was used to prepare the hydrophilic
nanocrystals. Analysis of the structural properties using powder X-ray
diffraction revealed that under the same experimental condition, the
plasmonic NPs altered the diffractive crystal structure of the alloyed
QDs. Depending on the fabrication strategy, the crystal nature of
OLA-AuNP-assisted CdZnSeS QDs was a pure hexagonal wurtzite domain
and a cubic zinc-blende domain, whereas the diffraction pattern of
OLA-AgNP-assisted CdZnSeS QDs was dominantly a cubic zinc-blende domain.
Insights into the growth morphology of the QDs revealed a steady transformation
from a heterogeneous growth pattern to a homogenous growth pattern
that was strongly influenced by the plasmonic NPs. Tuning the optical
properties of the alloyed QDs via plasmonic optical engineering showed
that the photoluminescence (PL) quantum yield (QY) of the AuNP-assisted l-cyst-CdZnSeS QDs was tuned from 10 to 31%, whereas the PL
QY of the AgNP-assisted l-cyst-CdZnSeS QDs was tuned from
15 to 90%. The low PL QY was associated with the surface defect state,
while the remarkably high PL QY exhibited by the AgNP-assisted l-cyst-CdZnSeS QDs lends strong affirmation that the fabrication
strategy employed in this work provides a unique opportunity to create
single ensemble, multifunctional, highly fluorescent alloyed QDs for
tailored biological applications
Purification of functional baculovirus particles from silkworm larval hemolymph and their use as nanoparticles for the detection of human prorenin receptor (PRR) binding
<p>Abstract</p> <p>Background</p> <p>Baculovirus, which has a width of 40 nm and a length of 250-300 nm, can display functional peptides, receptors and antigens on its surface by their fusion with a baculovirus envelop protein, GP64. In addition, some transmembrane proteins can be displayed without GP64 fusion, using the native transmembrane domains of the baculovirus. We used this functionality to display human prorenin receptor fused with GFP<sub>uv </sub>(GFP<sub>uv</sub>-hPRR) on the surface of silkworm <it>Bombyx mori </it>nucleopolyhedrovirus (BmNPV) and then tested whether these baculovirus particles could be used to detect protein-protein interactions.</p> <p>Results</p> <p>BmNPV displaying GFP<sub>uv</sub>-hPRR (BmNPV-GFP<sub>uv</sub>-hPRR) was purified from hemolymph by using Sephacryl S-1000 column chromatography in the presence of 0.01% Triton X-100. Its recovery was 86% and the final baculovirus particles number was 4.98 × 10<sup>8 </sup>pfu. Based on the results of enzyme-linked immunosorbent assay (ELISA), 3.1% of the total proteins in BmNPV-GFP<sub>uv</sub>-hPRR were GFP<sub>uv</sub>-hPRR. This value was similar to that calculated from the result of western blot by a densitometry (2.7%). To determine whether BmNPV-GFP<sub>uv</sub>-hPRR particles were bound to human prorenin, ELISA results were compared with those from ELISAs using protease negative BmNPV displaying β1,3-<it>N</it>-acetylglucosaminyltransferase 2 fused with the gene encoding GFP<sub>uv </sub>(GGT2) (BmNPV-<it>CP</it><sup>-</sup>-GGT2) particles, which do not display hPRR on their surfaces.</p> <p>Conclusion</p> <p>The display of on the surface of the BmNPV particles will be useful for the detection of protein-protein interactions and the screening of inhibitors and drugs in their roles as nanobioparticles.</p
Entrapment of calf-thymus DNA on magnetic nanoparticles
Magnetic nanoparticles can be used to load bio–molecules for various biophysical applications. The direct attachment of bio–molecules such as protein and DNA to magnetic nanoparticles may lead to alter their structure. A method to immobilize and store biomolecules for example DNA on to a magnetic nanoparticles surface without much structural alteration is carried out in the present work. DNA is a target for many therapeutic small molecules as therapeutic drugs bind to DNA – interfering with protein factors involved in DNA mechanism, or cleave DNA cross-link – interfering with the cell division. This may find applications in drug interactions study with the stored DNA without much alteration in their structure
Development of new coronavirus detection method using fluorescence enhancement by quantum dot-gold nanoparticle complex interaction
Development of new coronavirus detection method using fluorescence enhancement by quantum dot-gold nanoparticle complex interaction
A localized surface plasmon resonance-amplified immunofluorescence biosensor for ultrasensitive and rapid detection of nonstructural protein 1 of Zika virus
publishe
High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization
<p>Abstract</p> <p>Background</p> <p>Budded baculoviruses are utilized for vaccine, the production of antibody and functional analysis of transmembrane proteins. In this study, we tried to produce and purify the recombinant <it>Bombyx mori </it>nucleopolyhedrovirus (rBmNPV-hPRR) that displayed human (pro)renin receptor (hPRR) connected with FLAG peptide sequence on its own surface. These particles were used for further binding analysis of hPRR to human prorenin. The rBmNPV-hPRR was produced in silkworm larvae and purified from its hemolymph using size exclusion chromatography (SEC).</p> <p>Results</p> <p>A rapid method of BmNPV titer determination in hemolymph was performed using quantitative real-time PCR (Q-PCR). A correlation coefficient of BmNPV determination between end-point dilution and Q-PCR methods was found to be 0.99. rBmNPV-hPRR bacmid-injected silkworm larvae produced recombinant baculovirus of 1.31 × 10<sup>8 </sup>plaque forming unit (pfu) in hemolymph, which was 2.8 × 10<sup>4 </sup>times higher than transfection solution in Bm5 cells. Its purification yield by Sephacryl S-1000 SF column chromatography was 264 fold from larval hemolymph at 4 days post-injection (p.i.), but 35 or 39 fold at 4.5 or 5 days p.i., respectively. Protein patterns of rBmNPV-hPRR purified at 4 and 5 days were the same and ratio of envelope proteins (76, 45 and 35 kDa) to VP39, one of nucleocapsid proteins, increased at 5 days p.i. hPRR was detected in only purified rBmNPV-hPRR at 5 days p.i..</p> <p>Conclusion</p> <p>The successful purification of rBmNPV-hPRR indicates that baculovirus production using silkworm larvae and its purification from hemolymph by Sephacryl S-1000 SF column chromatography can provide an economical approach in obtaining the purified BmNPV stocks with high titer for large-scale production of hPRR. Also, it can be utilized for further binding analysis and screening of inhibitors of hPRR.</p
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