Endophytic actinomycetes from spontaneous plants of Algerian Sahara: indole-3-acetic acid production and tomato plants growth promoting activity

Twenty-seven endophytic actinomycete strains were isolated from five spontaneous plants well adapted to the poor sandy soil and arid climatic conditions of the Algerian Sahara. Morphological and chemotaxonomical analysis indicated that twenty-two isolates belonged to the Streptomyces genus and the remaining five were non- Streptomyces. All endophytic strains were screened for their ability to produce indole-3-acetic acid (IAA) in vitro on a chemically defined medium. Eighteen strains were able to produce IAA and the maximum production occurred with the Streptomyces sp. PT2 strain. The IAA produced was further extracted, partially purified and confirmed by thin layer chromatography (TLC) analysis. The 16S rDNA sequence analysis and phylogenetic studies indicated that strain PT2 was closely related to Streptomyces enissocaecilis NRRL B 16365T, Streptomyces rochei NBRC 12908T and Streptomyces plicatus NBRC 13071T, with 99.52 % similarity. The production of IAA was affected by cultural conditions such as temperature, pH, incubation period and L-tryptophan concentration. The highest level of IAA production (127 lg/ml) was obtained by cultivating the Streptomyces sp. PT2 strain in yeast extract-tryptone broth supplemented with 5 mg L-tryptophan/ ml at pH 7 and incubated on a rotary shaker (200 rpm) at 30°C for 5 days. Twenty-four-hour treatment of tomato cv. Marmande seeds with the supernatant culture of Streptomyces sp. PT2 that contained the crude IAA showed the maximum effect in promoting seed germination and root elongation

Determination of adsorption characteristics of synthetic NaX nanoparticles

Zeolite nanoparticles were synthesized by means of a hydrothermal method and the sorption performances were tested in batch experiments using single and binary components of Pb2+, Ni2+ and Cu2+ synthetic solutions. Fast adsorption was observed and the majority of sorption occurred within the first 5 min for each case of metal cations. Percentage metal removal increased with the adsorbent dosage and it was adversely affected by the initial metal concentrations. XRD, FTIR-ATR and XPS analyses revealed that the removal of metal ions occurred mainly on the basis of ion-exchange. Equilibrium sorption data were best described by Sips isotherm model and the maximum attainable metal amount on the NaX were estimated as 1.23, 1.76 and 2.20 mmol/g for the Pb2+, Ni2+, and Cu2+ cations, respectively. A single step desorption process conducted in NaCl solution seemed to be practically applicable for regeneration and after three adsorption/desorption cycles, 79, 76 and 48% of initial sorption capacity of NaX were preserved for lead, nickel and copper ions, respectively. Single isotherm parameters used for the prediction of binary equilibrium data were satisfactory. Binary sorption experiments for Pb2+-Ni2+, Pb2+-Cu2+ and Ni2+-Cu2+ couples reflected that the presence of secondary ions decreased the uptake of the primary one. Lead exhibited greater inhibition of the sorption of nickel and copper, demonstrating the stronger affinity of NaX for Pb2+. Extended Freundlich model best described all the three binary metal systems. Adsorption experiments carried out in real wastewater demonstrated that NaX nanoparticle has a high affinity for all the cations except copper. © 2019 Elsevier B.V

JOURNAL OF HAZARDOUS MATERIALS

In this study, the adsorption and the filtration processes were coupled by a zeolite nanoparticle impregnated polysulfone (PSf) membrane which was used to remove the lead and the nickel cations from synthetically prepared solutions. The results obtained from X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis indicated that the synthesized zeolite nanoparticles, using conventional hydrothermal method, produced a pure NaX with ultrafine and uniform particles. The performance of the hybrid membrane was determined under dynamic conditions. The results also revealed that the sorption capacity as well as the water hydraulic permeability of the membranes could both be improved by simply tuning the membrane fabricating conditions such as evaporation period of the casting film and NaX loading. The maximum sorption capacity of the hybrid membrane for the lead and nickel ions was measured as 682 and 122 mg/g respectively at the end of 60 min of filtration, under 1 bar of transmembrane pressure. The coupling process suggested that the membrane architecture could be efficiently used for treating metal solutions with low concentrations and transmembrane pressures, (C) 2016 Elsevier B.V. All rights reserved

Layer-by-layer self-assembly of multifunctional enzymatic UF membranes

In order to eliminate membrane fouling and to ensure enzymatic hydrolysis of urea, multifunctional biocatalytic membranes were prepared by using urease (URE) and trypsin (TRY) enzymes on the sulfonated polysulfone (SPSf) ultrafiltration membrane via layer-by-layer (LbL) self-assembly method. The membrane architecture consisted of multilayer assembly with TRY and URE enzymes as the outer layer and inner sandwiched layer, respectively. Polyethyleneimine (PEI) and alginate (ALG) were used as cationic and anionic polyelectrolytes. Sulfonation and PEI deposition were successfully accomplished as confirmed by attenuated total reflectance Fourier transform infrared spectroscopy, differential scanning calorimetry (DSC) and scanning electron microscopy analysis, contact angle measurements, staining with toluidine blue and Congo red dyes and dead-end filtration experiments. A characteristic value of SPSf membrane with a high water permeability (1000 L/m2.h.bar) and 95% bovine serum albumin (BSA) rejection was observed. In static conditions, URE activities of SPSf2-PEI-URE membrane were not affected by BSA fouling, while TRY immobilizations with increased concentrations (SPSf2-PEI-URE-PEI-ALG-TRY) significantly lowered the activity of URE. In dynamic conditions, each deposited layer exhibited individual resistance to flow that can be considered as irreversible fouling and caused 90% of flux decline for the SPSf2-PEI-URE-PEI-ALG-TRY membrane assembly. The recovery of the initial flux for the multilayered membrane at the end of six fouling and washing cycles was observed 85%. Moreover, at the end of 5 cycles, 78% of the URE initial activity of the multilayered membrane was preserved. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48750. © 2019 Wiley Periodicals, Inc

JOURNAL OF THE FACULTY OF ENGINEERING AND ARCHITECTURE OF GAZI UNIVERSITY

Adsorption is an effective method for the removing of heavy metals in the industrial waste water. Improvement of the efficiencies of the adsorbents can be possible by reduction of the particle size into nano scale. Therefore, in this study, zeolite nano-particles were synthesized by classical and microwave heating methods and then determined their adsorption capacities for the lead and nickel cations during batch adsorption processes carried out at 25 degrees C with different adsorbent loading and initial metal concentrations. Results obtained from X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis revealed that the synthesized zeolite nanoparticles was pure NaX with ultrafine and uniform particle sizes around 170 nm and Si/Al ratio of 1.24. Batch adsorption studies at 25 degrees C revealed that, NaX nanoparticles have high affinities against lead, nickel and methylene blue dye. As the initial metal and dye concentrations were increased, sorption capacity of the NaX was increased and reached the maximum value of 0.99, 1.33, 2.11, 1.89 and 0.15 mmol/g for the lead, nickel, copper, silver and methylene blue cations respectively. Sips model equation successfully described the equilibrium adsorption data of all adsorbates. Adsorption experiments of NaX for heavy metals from a real wastewater indicates that although the solution contains calcium and potassium ions in huge concentrations, the adsorbent is capable to adsorb lead and nickel even if they are low in concentrations

Relation Between Metabolic Parameters in 18F-FDG PET/CT and Hematological Markers in Non-Small Cell Lung Cancer

32nd Annual Congress of the European-Association-of-Nuclear-Medicine (EANM) -- OCT 12-16, 2019 -- Barcelona, SPAINWOS: 000492444405096[No abstract available]European Assoc Nucl Me