In vitro susceptibility of gram-negative bacterial isolates to chlorhexidine gluconate

Objective: To investigate the susceptibility of clinical isolates of gram-negative bacteria to chlorhexidine gluconate. Design: Prospective laboratory study. Setting: Tikur Anbessa Hospital, Addis Ababa, Ethiopia. Subjects: Clinical specimens from 443 hospital patients. Main outcome measures: Significant number of gram negative bacteria were not inhibited by chlorhexidine gluconate (0.02-0.05%) used for antisepsis. Results: Four hundred and forty three strains of gram-negative bacteria were isolated from Tikur Anbessa Hospital patients. Escherichia coli (31.6%) and Klebsiella pneumoniue (23%) were the most frequently isolated bacteria followed by Proteus species (13.3%), Pseudomonas species (9.2%), and Citrobacter species (6.1%). Each organism was tested to chlorhexidine gluconate (CHG), minimum inhibitory concentration (MIC) ranging from 0.0001% to l%w/v. All Salmonella species and E. coli were inhibited by CHG, MIC I 0.01%. Twenty nine per cent of Acinetobacter, 28% of K. pneumoniae and Enterobacter species and 19-25% of Pseudomonas, Proteus and Providenciu species were only inhibited at high concentrations of CHG (2 0.1%). Conclusion: Our results showed that a significant number of the gram-negative bacterial isolates were not inhibited by CHG at the concentration used for disinfection of wounds or instruments (MIC 0.02-0.05% w/v). It is therefore important to select appropriate concentration of this disinfectant and rationally use it for disinfection and hospital hygiene. Continuing follow up and surveillance is also needed to detect resistant bacteria to chlorhexidine or other disinfectants in time.East African Medical Journal, May 1999, 234-24

Evaluation of the performance of bias-corrected CORDEX regional climate models in reproducing Baro–Akobo basin climate

The applicability of the regional climate model (RCMs) for catchment hydroclimate is obscured due to their systematic bias. As a result, bias correction has become an essential precondition for the study of climate change. This study aimed to evaluate the skill of seven rainfall and five maximum and minimum temperature RCM outputs against observed data in simulating the characteristics of climate at several locations over the Baro–Akobo basin in Ethiopia. The evaluation was performed based on raw and bias-corrected RCMs against observed for a long-term basis. Several statistical metrics were used to compare RCMs against observed using a pixel-to-point approach. In this finding, raw RCMs showed pronounced biases such as lower correlation and higher PBIAS in estimating rainfall and minimum temperature than maximum temperature. However, most RCMs after bias correction showed better performance in reproducing the magnitude and distribution of the mean monthly rainfall and temperature and improve all the statistical metrics. The Mann–Kendall trend test for observed and bias-corrected RCMs indicated a decreasing annual rainfall trend while the maximum and minimum temperature showed an increasing trend in most stations. In most statistical metrics, the ensemble mean resulted in better agreement with observation than individual models in most stations. In general, after bias correction, the ensemble adequately simulates the Baro–Akobo basin climate and can be used for evaluation of future climate projections in the region

A crowdsourcing approach to detect farmers’ preferences: evidences from Ethiopia for adapting to climate change

Climate change is severely affecting production systems all over Africa. Ethiopia will face an increase in temperature and changes in rainfall patterns. One solution for long term management of climate related risks is to introduce new traits into production systems. We present an approach to quickly deliver to farmers a selected number of preferred accessions of durum wheat using a crowdsourcing approach. After having completed a phenotypic and genotypic characterisation of 400 accessions of Ethiopian landraces and asked farmers to evaluate them, we distributed seeds of preferred varieties to a large number of farmers using a crowdsourcing approach. In two sites representing different agroecological zones, we distributed 20 superior varieties and one check, an improved variety very common in both areas to 200 farmers in 12 villages per site, covering an area of roughly 350 km 2. We repeated the experiment for 2 years. Each farmer was given 3 blind varieties and the check, each variety being equally represented in the sample. In addition, in each village we included 2 i-buttons, measuring temperature and humidity every 3 hours throughout the growing season. This allowed to analyse the data on critical climatic parameters. Results clearly indicate farmers’ preferences: a) Landraces were preferred over improved varieties for their multiple uses; b) By considering farmers as citizen scientists, we have a better understanding of the criteria farmers use to select their preferred varieties (in our case straw and grain yield, drought tolerance, uniformity, long and dense spikes). In conclusion: • This process has been very effective in quickly disseminating seeds that match farmers’ needs. After 2 years several hundred farmers can use better adapted material. • It shows the potential of landraces to provide immediate option for managing climate related risks and calls for broader use of material conserved in gene banks. • It indicates the need to strengthen local seed systems to better manage these resources. • It indicates how farmers can provide very valuable scientific information that can be translated into research as well as development potential in other areas of research

High-density molecular characterization and association mapping in Ethiopian durum wheat landraces reveals high diversity and potential for wheat breeding

Durum wheat (Triticum turgidum subsp. durum) is a key crop worldwide, and yet, its improvement and adaptation to emerging environmental threats is made difficult by the limited amount of allelic variation included in its elite pool. New allelic diversity may provide novel loci to international crop breeding through quantitative trait loci (QTL) mapping in unexplored material. Here, we report the extensive molecular and phenotypic characterization of hundreds of Ethiopian durum wheat landraces and several Ethiopian improved lines. We test 81 587 markers scoring 30 155 single nucleotide polymorphisms and use them to survey the diversity, structure, and genome-specific variation in the panel. We show the uniqueness of Ethiopian germplasm using a siding collection of Mediterranean durum wheat accessions. We phenotype the Ethiopian panel for ten agronomic traits in two highly diversified Ethiopian environments for two consecutive years and use this information to conduct a genome-wide association study. We identify several loci underpinning agronomic traits of interest, both confirming loci already reported and describing new promising genomic regions. These loci may be efficiently targeted with molecular markers already available to conduct marker-assisted selection in Ethiopian and international wheat. We show that Ethiopian durum wheat represents an important and mostly unexplored source of durum wheat diversity. The panel analysed in this study allows the accumulation of QTL mapping experiments, providing the initial step for a quantitative, methodical exploitation of untapped diversity in producing a better wheat

Socioeconomic Profile and Gender Characteristics in Relation to Camel Management Practices in the Pastoral Communities of Ethiopia

Pastoralism was one of the oldest forms of organized human society in providing sustainable livelihood opportunities. However, this way of life is increasingly under threat due to a number of reasons. Today’s political, legal, social and economic policies and laws are not taking into consideration the needs of pastoral populations specially women and children. An attempt has been made to evaluate the socio-economic conditions among the pastoral households of Eastern and Southern parts of Ethiopia. The study involved three major pastoral areas of Ethiopia, namely, Somali, Afar and Oromiya regional states. The findings of this study revealed that lacking of formal education except limited religious education for male children, girl children are excluded from any type of literacy. Population size in the study areas reflects above national average, due to custom of polygon. Regionally there has been a variation in decision making on the issues like intensity of production, breeding, camel herding, milking, marketing and other related activities. In general this study observed that there has been a dominant role played by men in most of the revenue generating activities except in few areas of districts other than economic activities. Hence, different types of social and policy intervention should be made. Keywords: Pastoralism, education, Polygon, intensity of production, Breeding

Sizing ice hydrometeor populations using the dual-wavelength; radar ratio

Dual-wavelength (3.2 and 0.32&thinsp;cm, i.e., X- and W-radar bands) radar ratio (DWR) measurements in ice clouds and precipitation using Canada's National Research Council Institute for Aerospace Research airborne radar are compared to closely collocated particle microphysical in&nbsp;situ sampling data in order to develop relations between DWR and characteristic hydrometeor size. This study uses the radar and in&nbsp;situ data sets collected during the In-Cloud ICing and Large-drop Experiment (ICICLE) campaign in midlatitude frontal clouds. Since atmospheric particle scattering at X band is predominantly in the Rayleigh regime and the W-band frequency is the highest frequency usually used for hydrometeor remote sensing, the X&ndash;W-band combination provides a relatively strong dual-wavelength reflectivity difference. This study considers radar and in&nbsp;situ measurements conducted in relatively homogeneous cloud and precipitation conditions. Measurements show that under these conditions, the difference between the X-band radar reflectivities observed with vertical and horizontal pointing of the radar beam are generally small and often negligible. However, W-band reflectivities at vertical beam pointing are, on average, larger than those for horizontal beam pointing by about&nbsp;4&thinsp;dB, which is a non-Rayleigh scattering effect from preferentially oriented non-spherical particles. A horizontal radar beam DWR&ndash;mean volume particle size relation, Dv, provides robust estimates of this characteristic size for populations of particles with different habits. Uncertainties of Dv retrievals using DWR are around 0.6&thinsp;mm when Dv is greater than approximately 1&thinsp;mm. Size estimates using vertical radar beam DWRs have larger uncertainties due to smaller dual-wavelength signals and stronger influences of hydrometeor habits and orientations at this geometry of beam pointing. Mean relations among different characteristic sizes, which describe the entire particle size distribution (PSD), such as Dv, and other sizes used in various applications (e.g., the mean, effective, and median sizes) are derived, so the results of this study can be used for estimating different PSD characteristic sizes. &nbsp;</p

Multi-Objective Optimization of Transonic Compressor Blade Using Evolutionary Algorithm

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/77106/1/AIAA-14667-294.pd

Genetic diversity and population structure among sorghum (Sorghum bicolor, L.) germplasm collections from Western Ethiopia

The Western Ethiopian region harbors a unique set of sorghum germplasm adapted to conditions not conventional to sorghums grown in other parts of the world. Accessions from the region possess unique resistance to multiple leaf and grain diseases. This study is aimed at exploring the extent of genetic variation and population structure among accessions of this region. A total of 123 accessions comprising 111 from Western Ethiopia (62 from Asosa and 49 from Pawe) and 12 U.S. adapted lines were genotyped using 30 sorghum simple sequence repeat markers (SSR). Genetic diversity and population structure were analyzed using PowerMarker and STRUCTURE software, respectively, based on 23 polymorphic SSR markers. Principal component analysis (PCA) was performed to view the variability in multi-dimensional space. Population structure analysis revealed considerable admixtures between Pawe and Asosa accessions, while the PowerMarker analysis grouped the accessions into three distinct clusters largely based on collection regions. The PCA did not clearly differentiate Asosa and Pawe accessions, but U.S. adapted lines were clearly separated from the rest. The study indicated the presence of marked genetic variability among accessions from Western Ethiopia and also provided clues on shared genetic events among accessions adapted to the two areas in Western Ethiopia.Keywords: Sorghum, genetic diversity, population structure, SSR, Ethiopi

Milk productivity of camel and growth of calves (Camelusdromedarius) in eastern Ethiopia

Milk suckled, off take and growth of calves of Camel was measured from August 2008 to July 2009 in eastern Ethiopia with the aim to evaluate the performance of the herd in terms of milk productivity and growth of calves. Data on milk suckled and growth of calves was collected by methods of weight-suckle-weight. Milk off take of the herd was measured by hand milking method. Productivity of the herd in terms of milk was evaluated based on the sum of milk suckled and off take. Average daily milk suckled was 2.00 ± 0.18 kg, daily milk off take was 3.50 ± 0.14 liter, daily weight gains of calves was 0.243 ± 0.01 kg/day, respectively. Average milk productivity as the sum of milk suckled and off take was 5.5 ± 0.16. Milk sucked, off take and daily weight gain of calves was affected by months of lactation, season of births of calves and parities of dams. Daily milk suckled, off take and weight gain in 3-4 months of lactation was relatively higher than other months of lactation which were 2.17 + 0.22 kg, 3.55± 0.16 liter and 0.300 ± 0.02 kg, respectively. The productivity of the herd in terms of milk yield and growth of calves decrease as the camels advanced in lactation. Daily milk suckled, off take and weight gain was relatively lower in 11-12 months of lactation which was 1.27+ 0.31 kg, 1.69 ± 0.15 liter and 0.193± 0.02 kg, respectively. From the study it was concluded that the Camel herd in eastern Ethiopia managed under arid range condition was comparable to the productivity of Camel in east Africa based on the performance measured in terms of milk productivity and growth of calves.National Camel Research Project and University of Haramayahttp://www.lrrd.org/hb201