1,834 research outputs found
Development of Microsatellite Markers for Loxosceles devia Gertsch & Mulaik (Araneae: Sicariidae) using Next Generation Sequencing
Brown recluse spiders in the genus Loxosceles are known for causing severe necrotic skin lesions. Despite their impact on human health, very little is known concerning the genetic diversity or population structure of this genus. The development of a suite of molecular markers (microsatellites) would allow us to obtain genetic data and expand the resources available to study Loxosceles devia, the species found throughout south Texas. Microsatellites are long tandem repeats about 1-6 nucleotides long of DNA, also known as simple sequence repeats. Their co-dominant method of inheritance, wide spread distribution across the nuclear genome, ease of scoring, and high mutation rates make microsatellites an ideal molecular marker. Unfortunately, the cost of developing microsatellites has limited their availability until now. The Ion Torrent Personal Genome Machine® was used to shotgun sequence libraries of genomic DNA derived from L. devia. The program MSATCOMMANDER™ was used to identify and design primers for di-, tri-, and tetra- microsatellite repeats from these shotgun sequences. Seventeen microsatellite markers were designed and polymerase chain reaction conditions were optimized for each primer to develop a suite of novel molecular markers for this species
Análisis cuantitativo del precio de la vivienda en Córdoba
El precio de la vivienda en zonas urbanas se analiza con un enfoque de
modelos hedónico, en el que se valoran las caracterÃsticas que determinan el
precio que adquiere este bien en el mercado. Además de definir una serie de
Ãndices que tratan de representar caracterÃsticas cualitativas de cada finca urbana,
se analizan e interpretan los componentes principales con datos muestrales tomados
en la ciudad de Córdoba, elaborándose el correspondiente modelo de determinación
del precio de una vivienda
Characteristics of GaN Thin Films Using Magnetron Sputtering System
The paper presents a polycrystalline GaN thin film with a hexagonal wurtzite structure under the optimized sputtering conditions of 40 W RF power, 5 mT working pressure, using pure nitrogen gas with a substrate temperature of 700°C. The study examines the effects of surface disorders and incorporates it in the thin films characteristics. A radio frequency (RF) Ultra High Vacuum (UHV) Magnetron Sputtering System has been used for the deposition of Gallium Nitride (GaN) on silicon, sapphire and glass substrates with different parameters. The power is varied from 40 W to 50 W, and the pressure from 4 mT to 15 mT. The effects of the RF sputtering powers and gas pressures on the structural properties are investigated experimentally. Sputtering at a lower RF power of 15 W does increase the N atomic percentage, however the deposition rate is substantially slower and the films are amorphous. GaN deposited on both silicon and sapphire wafer resulted in thin films close to stoichiometric once the N2 concentration is 60% or higher. It is also observed that the substrate cooling/heating effects improve the quality of the thin films with fewer defects present at the surface of the GaN epi-structure
Biohydrogen production from diary processing wastewater by anaerobic biofilm reactors
Fermentative hydrogen production was studied in packed bed batch reactors to assess the influence of environmental factors over yield hydrogen production from dairy wastewater. Dried stems of Opuntia imbricata were used as substratum adding a pretreated mixed culture for biofilm formation. Experimental results showed that, yield hydrogen production was significantly affected by initial COD concentration, temperature and dairy wastewater pH. Maximum yield obtained was 12.73 mM H2/g CODc when initial COD concentration was 21.1 g COD, dairy wastewater pH with no adjustment (11.32) and room temperature of 16 ± 3°C. Methane production was completely inhibit at an initial pH of 4 at all temperature studied (final pH 4.06), meanwhile, with an initial pH of 11.32, with exception for 16°C, methanogenic activity was not completely inhibit when final pH was over 5, showing an increase in methane production of 0.35 to 0.75 g CH4/l for 35 to 55°C.Key words: Biofilm, dairy wastewater, hydrogen, Opuntia imbricat
Análisis literario : colección de trozos y composiciones en prosa y verso de los clásicos castellanos
Copia digital. Valladolid : Junta de Castilla y León. ConsejerÃa de Cultura y Turismo, 2009-201
El estilo y el lenguaje : discurso leÃdo en la solemne apertura del curso 1884 a 1885 en el Instituto de Segovia
Copia digital. Valladolid : Junta de Castilla y León. ConsejerÃa de Cultura y Turismo, 2009-201
Does the Integration of Lean Production and Industry 4.0 in Green Supply Chains Generate a Better Operational Performance?
Purpose – The tightening of environmental measures and policies in various countries around the world is forcing manufacturing companies, particularly those that make up the automotive industry, to improve their production processes, through the implementation of approaches such as lean production and industry 4.0 technologies, to reduce industrial waste. However, the literature indicates that the implementation of lean production and Industry 4.0 does not always lead to an improvement in the level of operational performance. Therefore, this study analyzes the effects of the implementation of lean production practices and Industry 4.0 on a green supply chain and the operational performance of manufacturing companies in the Mexican automotive industry.
Methodology/design/approach - A theoretical research framework consisting of six hypotheses was developed and validated by applying PLS-SEM and using a sample of 460 companies from the Mexican automotive industry.
Findings - The results show that the level of operational performance of manufacturing companies increases substantially with the implementation of lean production and industry 4.0 practices, as well as a green supply chain.
Originality/value - This study contributes to the literature on lean production and Industry 4.0 by providing robust empirical evidence of the positive effects of implementing these approaches on the green supply chain and operational performance of manufacturing companies.
Practical implications - Managers of manufacturing companies will be able to use the results of this study to improve their production systems and to demonstrate the effects of these practices on operational performance
Investigating Potential Interventions on disruptive impacts of Industry 4.0 technologies in Circular Supply chains: Evidence from SMEs of an Emerging Economy
As a transversal theme, the intertwining of digitalization and sustainability has crossed all Supply Chains (SCs) levels dealing with widespread environmental and societal concerns. This paper investigates the potential interventions and disruptive impacts that Industry 4.0 technologies may have on pharmaceutical Circular SCs (CSCs). To accomplish this, a novel method involving a literature review and Pythagorean fuzzy-Delphi has initially been employed to identify and screen categorized lists of Industry 4.0 Disruptive Technologies (IDTs) and their impacts on pharmaceutical CSC. Subsequently, the weight of finalized impacts and the performance score of finalized IDTs have simultaneously been measured via a novel version of Pythagorean fuzzy SECA (Simultaneously Evaluation of Criteria and Alternatives). Then, the priority of each intervention for disruptive impacts of Industry 4.0 has been determined via the Hanlon method. This is one of the first papers to provide in-depth insights into advancing the study of the disruptive action of Industry 4.0 technologies cross-fertilizing CE throughout pharmaceutical SCs in the emerging economy of Iran. The results indicate that digital technologies such as Big Data Analytics, Global Positioning Systems, Enterprise Resource Planning, and Digital Platforms are quite available in the Irans' pharmaceutical industry. These technologies, along with four available interventions, e.g., environmental regulations, subsidy, fine, and reward, would facilitate moving towards a lean, agile, resilient, and sustainable supply chain through the efficient utilization of resources, optimized waste management, and substituting the human workforce by machines
Supplier selection for smart supply chain: An adaptive fuzzy-neuro approach
In recent years, companies have experienced international changes that have occurred as a result of technological advances, market globalization, or natural disasters. So, organizations are trying to improve their performance in order to be more competitive. In other words, organizations’ competitiveness highly depends on their suppliers. At present, companies need to consider and include so-called ‘resilience’, ‘sustainability’, and ‘smartness’ in the supplier’s selection to retain a competitive advantage. In this context, the purpose of this paper is to present an intelligent decision-making model for selecting the appropriate suppliers. For doing so, a set of criteria evaluation was determined to respond to the novel era circumstances. The suggested work is helpful for academics as well as professionals as it emphasizes the importance of resilient-sustainable supplier selection in the digital era.N/
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