El cautivo ; La violeta

Suplemento de "Misión" (n.175, 1938)Copia digital. Valladolid : Junta de Castilla y León. Consejería de Cultura y Turismo, 2009-201

Controlling spatiotemporal pattern formation in a concentration gradient with a synthetic toggle switch.

The formation of spatiotemporal patterns of gene expression is frequently guided by gradients of diffusible signaling molecules. The toggle switch subnetwork, composed of two cross-repressing transcription factors, is a common component of gene regulatory networks in charge of patterning, converting the continuous information provided by the gradient into discrete abutting stripes of gene expression. We present a synthetic biology framework to understand and characterize the spatiotemporal patterning properties of the toggle switch. To this end, we built a synthetic toggle switch controllable by diffusible molecules in Escherichia coli. We analyzed the patterning capabilities of the circuit by combining quantitative measurements with a mathematical reconstruction of the underlying dynamical system. The toggle switch can produce robust patterns with sharp boundaries, governed by bistability and hysteresis. We further demonstrate how the hysteresis, position, timing, and precision of the boundary can be controlled, highlighting the dynamical flexibility of the circuit

MPI Collective Operations over IP Multicast

Many common implementations of Message Passing Inter- face (MPI) implement collective operations over point-to-point operations. This work examines IP multicast as a framework for collective operations. IP multicast is not reliable. If a receiver is not ready when a message is sent via IP multicast, the message is lost. Two techniques for ensuring that a message is not lost due to a slow receiving process are examined. The techniques are implemented and compared experimentally over both a shared and a switched Fast Ethernet. The average performance of collective operations is improved as a function of the number of participating processes and message size for both networks