Antifungal and Bioherbicidal Properties of Essential Oils of Thymus fallax Fish & Mey., Origanum vulgare L. and Mentha dumetorum Schult.

WOS: 000343767000048The chemical composition of the essential oils obtained from the aerial parts of Thymus fallax, Origanum vulgare and Mentha dumetorum was analyzed by gas chromatography-mass spectrometry and the following were found to be the main constituents: T. fallax-thymol (41.48 %), o-cymene (26.75 %), zeta-terpinen (15.84 %), 2-isopropyl-1-methoxy-4-methylbenzene (5.10 %), terpineolene (2.11 %) and carvacrol (1.28 %); O. vulgare-thymol (50.41 %), carvacrol (12.96 %), 2-bornene (11.28 %), zeta-terpinen (8.80 %), o-cymene (6.68 %), alpha-bisabolane (2.19 %) and caryophyllene (1.31 %); and M. dumetorum-carvone (39.64 %), eucalyptol (14.34 %), dihydrocarvone (12.78 %), limonene (7.79 %). The antifungal activities of the oils against Alternaria solani, Fusarium oxysporum and Rhizoctonia solani were also evaluated and were found to be toxic to the pathogens. The results revealed that essential oils, especially those of T. fallax and O. vulgare, had a strong antifungal activity with a significant inhibition on the growth of the 3 tested fungi. In contrast, the M. dumetorum oil did not inhibit the growth of Rhizoctonia solani and also exerted a limited inhibitory effect on the mycelial growth of the other two fungi tested. The results of herbicidal assays using these essential oils against four different plant species, Abutilon theophrasti Medik., Agrostemma githago L., Medicago sativa L. and Lepidium sativum L., showed that the oils had inhibitory effects on seed germination and seedling growth. The findings of the present study confirmed that plant essential oils can be used as natural herbicides and fungicides to control weeds and pathogenic fungi, thus, reducing the dependence on synthetic pesticides

HEPATOCYTE APOPTOSIS INDUCTION BY ACETAMINOPHEN THROUGH MODULATION OF CASPASE/BAX PATHWAY IN MICE

Objective: Acetaminophen (APAP) overdose contributes to liver damage through modulation of pro-apoptotic processing. This study evaluated the involvement of caspase/Bax factors in APAP hepatotoxicity in vivo and in vitro. Methods: The involvement of caspase/Bax factors in APAP hepatotoxicity was evaluated in BALB/c mice and on isolated primary mouse hepatocytes. In vitro MTT assay was carried out on primary cultured mouse hepatocytes treated with APAP (2.5, 5, 10 mmol) and Annexin V/PI staining was employed to cell suspension for imaging under fluorescence microscopy. In addition, caspase-3 concentrations were determined in cell lysates. In vivo, mice were treated with a toxic dose of APAP (700 mg/kg) and immunodetection of Bax was made by Western Blot. Vitamin C (Vit C) was used as a hepato-protectant due to its known antioxidant activities. Results: In vitro dose-dependent increase in mitochondrial electron transport capacity was evident in isolated mouse primary hepatocytes incubated with the high dose of APAP (10 mmol) compared to both nontreated cells and cells pre-treated with Vitamin C (Vit C) (0.5 mmol) (p<0.05). Apoptosis was confirmed in hepatocytes through Annexin V staining after APAP treatment and the signal was reduced when hepatocytes were pre-treated with Vit C. In addition, caspase-3 concentration was decreased in cells pretreated with Vit C prior to APAP exposure. In vivo, Bax immunodetection utilizing western blotting was increased in mice treated with the toxic dose of APAP (700 mg/kg) and attenuated through pre-treatment with Vit C. Conclusion: Modulation of apoptotic caspase/Bax pathway is present in hepatocytes undergoing APAP-induced toxicity

SPATIAL DISTRIBUTION OF ATMOSPHERIC POLLUTION IN DUHOK URBAN AREA BY USING GIS TOOLS

Air pollution is becoming a serious challenge in thickly populated areas in the world. The air pollution in Kurdistan region is clearly noticed, especially in areas where pollution sources and human population are concentrated. Urbanization and economic growth are proceeding at a rapid pace, accompanied by increasing emissions of a mixture of greenhouse gases especially from transporting sector, that positively contribute to accelerate climate change around the globe, and also have impacts on public health and vegetation. In order to decrease the effects that caused by atmospheric pollution, suitable monitoring systems are urgently needed that can rapidly and reliably detect and quantify polluting sources and concentration for monitoring by local authorities in order to restrain more damage of the current pollution levels. In this study, GIS with portable gas detector (K-60 IV) KELISAIKE safety equipment, China, have been used to assess the status of NOx, VOCs and noise pollution at 54 randomly selected urban locations of Duhok city. The measurements were taken during the week and weekend days started from 9 am until 4 pm. The results showed that there is a variation in the values of the three variables NOx, VOCs and noise, where the highest values recorded during working days in the locations distributed along the highway and the city center with heavy traffic load and dense human population in comparison to lowest values obtained during weekend days in locations distributed outskirt of the city with lower population and traffic loads. Therefore, likely such connections exist between the urban traffic density and  low air pollution quality within urban locations around Duhok city

The Importance of Preventive Medicine in Family Practice: A Review of Current Guidelines and Recommendations

Prevention is seen as a critical topic in family practice. Primordial prevention, primary prevention, secondary prevention, tertiary prevention, and quaternary prevention are all part of this strategy to disease prevention. To avoid the formation and development of risk factors, primary prevention focuses on addressing the fundamental causes and social determinants of disease. Primary prevention is the practice of preventing illnesses before they arise via the use of treatments such as immunizations and health education. Secondary prevention focuses on illness identification and intervention as early as possible to avoid disease development. Tertiary prevention addresses illness outcomes by restoring health and offering rehabilitation. Finally, quaternary prevention seeks to safeguard patients against needless medical treatments and the harm caused by over-medicating. Risks frequently rise in tandem with frailty and comorbidities. In contrast, advantages frequently drop as life expectancy increases. Preventive management strategies should consider the patient's viewpoint and be mutually agreed upon. Healthcare providers must prioritize the deployment of preventive care services, even when clinical treatments are required, in order to overcome preventive care hurdles. Healthcare practitioners may play a critical role in illness prevention and contribute to family well-being by investing in preventive care and executing these measures

Applying Harmonic Optical Microscopy for Spatial Alignment of Atrial Collagen Fibers

BACKGROUND: Atrial fibrosis creates a vulnerable tissue for atrial fibrillation (AF), but the spatial disarray of collagen fibers underlying atrial fibrosis is not fully elucidated. OBJECTIVE: This study hypothesizes that harmonics optical microscopy can illuminate the spatial mal-alignment of collagen fibers in AF via a layer-by-layer approach. PATIENTS AND METHODS: Atrial tissues taken from patients who underwent open-heart surgery were examined by harmonics optical microscopy. Using the two-dimensional Fourier transformation method, a spectral-energy description of image texture was constituted and its entropy was used to quantify the mal-alignment of collagen fibers. The amount of collagen fiber was derived from its area ratio to total atrial tissue in each image. Serum C-terminal pro-collagen pro-peptide (CICP), pro-matrix metalloproteinase-1 (pro-MMP-1), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) were also evaluated. RESULTS: 46 patients were evaluated, including 20 with normal sinus rhythm and 26 with AF. The entropy of spectral-energy distribution of collagen alignment was significantly higher in AF than that in sinus rhythm (3.97 ± 0.33 vs. 2.80 ± 0.18, p<0.005). This difference was more significant in the permanent AF group. The amount of collagen was also significantly higher in AF patients (0.39 ± 0.13 vs. 0.18 ± 0.06, p<0.005) but serum markers of cardiac fibrosis were not significantly different between the two groups. CONCLUSIONS: Harmonics optical microscopy can quantify the spatial mal-alignment of collagen fibers in AF. The entropy of spectral-energy distribution of collagen alignment is a potential tool for research in atrial remodeling

Community pharmacists’ perspectives on cardiovascular disease pharmaceutical care in the United Arab Emirates: a questionnaire survey-based analysis

Background: Community pharmacists play an intermediary role between prescribing physicians and patients in the United Arab Emirates (UAE) and thus are responsible for ensuring that patients receive optimal cardiovascular disease (CVD) pharmaceutical care.Methods: we used a cross-sectional design to assess the perceptions and practices of community pharmacists concerning pharmaceutical care for patients with CVD. A trained researcher visited randomly selected community pharmacies and used a structured questionnaire to conduct in-person interviews with pharmacists. The questionnaire collected demographic data and information on perceptions and practices regarding CVD pharmaceutical care.Results: Five hundred and fifty-one participants were recruited. The average participant age (mean ± SD) was 35 ± 2.7 years. The average perception score regarding CVD prevention and management was 75.6% (95% confidence interval [CI] 77.1%–74.2%), and the average practice score for CVD prevention and management was 87.1% (95% CI 76.5%–79.6%). Bivariate analysis revealed that gender (p = 0.001), education level (p &lt; 0.001), pharmacy position (p = 0.004), work experience (p &lt; 0.001), number of patients served per day (p &lt; 0.001) and being trained on CVD prevention and management (p &lt; 0.001) were significantly associated with perceptions about the prevention and management of CVD. Better practice scores were seen among older participants (OR 1.01; 95% CI 1–1.019), postgraduates (OR 1.77; 95% CI 1.66–1.89), workers at chain pharmacies (OR 1.24; 95% CI 1.11–1.39), pharmacists in charge (OR 1.22; 95% CI 1.01–1.47), pharmacists with &gt;10 years of experience (OR 11.3; 95% CI 6.01–15.62), pharmacists with 6–10 years of experience (OR 4.42; 95% CI 3.90–5) and pharmacists trained on CVD prevention and management (OR 1.29; 95% CI 1.15–1.46).Conclusion: Pharmacy practitioners working in community pharmacies in the UAE actively engage in delivering pharmaceutical care to patients, playing a role in CVD management and prevention. However, they showed low levels of involvement in other healthcare services, specifically in screening and measuring patients’ weight, glucose levels, and blood pressure, monitoring treatment responses, maintaining medical records, and reviewing medication refill histories. Activities such as educating patients, providing medication counseling, offering support for treatment adherence, and fostering collaborative relationships with other healthcare providers should be encouraged among UAE community pharmacists to ensure the provision of high-quality patient care

Chemical composition and antifungal activity of the plant extracts of turkey cardaria draba (L.) desv

The main components of Cardaria draba (L.) Devs. essential oils were chemically analysed and identified by GC-MS. They were: 5-(methylthio)-pentanenitrile (41.13%), decane (11.40%) and nonane (10.93%). Antifungal activity of C. draba methanol and aquatic extracts against the plant pathogens Fusarium oxysporum f. sp. lycopersici (FOL), Alternaria solani, Verticilium dahliae, Rhizoctonia solani, and Sclerotinia sclerotiorum in vitro was determined. Various volumes of methanol and aquatic extracts were mixed with the sterile PDA to obtain different concentrations. In order to examine the used plant extracts, percentages of mycelium inhibition (MGI) values were calculated to compare with the positive control (80% Thiram), which is a standard fungicide. The used plant extract were showed significant antifungal activity against plant pathogenic fungi. © 2016 Egyptian Society for Biological Control of Pests. All rights reserved