Information technology performance measurement and improvement recommendation in Indonesian retail company

Information technology should work according to the needs and provide added value to the business. If the application of information technology does not provide added value to the business, information technology will only become a burden for the company. Therefore, it is necessary to measure performance to see to what extent the application of IT can support business processes and provide added value. This paper provides measurements and recommendations on IT governance in one of leading retail company in Indonesia. This research used descriptive quantitative research methods and IT Balanced Scorecard method that can provide an overview of IT performance in an organization based on four perspectives, such as Corporate Contribution, Customer Orientation, Operational Improvement, and Future Orientation. Based on the results of the analysis and measurement, the overall IT performance score was 62.64% where the score is in the “Moderate†category. The company contribution perspective got a score of 68.50%, the user orientation perspective was 63.00%, the operational improvement perspective was 62.06%, and the future orientation perspective was 57.44%. Several recommendations were constructed based on the consideration of the KPI value that must be improved. This can be a guide for other retail companies in formulating policies related to IT governance and enriching research in the field of IT performance measurement

Regulatory T Cells Expanded from Hiv-1-Infected Individuals Maintain Phenotype, Tcr Repertoire and Suppressive Capacity

While modulation of regulatory T cell (Treg) function and adoptive Treg transfer are being explored as therapeutic modalities in the context of autoimmune diseases, transplantation and cancer, their role in HIV-1 pathogenesis remains less well defined. Controversy persists regarding their beneficial or detrimental effects in HIV-1 disease, which warrants further detailed exploration. Our objectives were to investigate if functional CD4+ Tregs can be isolated and expanded from HIV-1-infected individuals for experimental or potential future therapeutic use and to determine phenotype and suppressive capacity of expanded Tregs from HIV-1 positive blood and tissue. Tregs and conventional T cell controls were isolated from blood and gut-associated lymphoid tissue of individuals with HIV-1 infection and healthy donors using flow-based cell-sorting. The phenotype of expanded Tregs was assessed by flow-cytometry and quantitative PCR. T-cell receptor ß-chain (TCR-β) repertoire diversity was investigated by deep sequencing. Flow-based T-cell proliferation and chromium release cytotoxicity assays were used to determine Treg suppressive function. Tregs from HIV-1 positive individuals, including infants, were successfully expanded from PBMC and GALT. Expanded Tregs expressed high levels of FOXP3, CTLA4, CD39 and HELIOS and exhibited a highly demethylated TSDR (Treg-specific demethylated region), characteristic of Treg lineage. The TCRß repertoire was maintained following Treg expansion and expanded Tregs remained highly suppressive in vitro. Our data demonstrate that Tregs can be expanded from blood and tissue compartments of HIV-1+ donors with preservation of Treg phenotype, function and TCR repertoire. These results are highly relevant for the investigation of potential future therapeutic use, as currently investigated for other disease states and hold great promise for detailed studies on the role of Tregs in HIV-1 infection.Elizabeth Glaser Pediatric AIDS Foundation (Pediatric HIV Vaccine Program Award MV-00-9-900-1429-0-00)Massachusetts General Hospital. Executive Committee on Research (MGH/ECOR Physician Scientist Development Award)National Institutes of Health (U.S.) (NIH NIAID (KO8 AI074405))National Institutes of Health (U.S.) (NIH NIAID AI074405-03S1)Massachusetts General Hospital (William F. Milton Fund)Harvard University. Center for AIDS Research (CFAR Scholar Award)Massachusetts General Hospital. Center for the Study Inflammatory Bowel Disease (P30DK043351)Harvard University. Center for AIDS Research (NIH funded program (5P30AI060354-09

Seni Memuridkan yang Bermakna dan Berbuah Berdasarkan 2 Timotius 2:1-2

Pemuridan adalah harga mati dari seorang murid Yesus. Murid Yesus yang sejati harus menghasilkan murid lainnya bagi Yesus. Pola pemuridan yang benar dan efektif yang diteladankan Yesus, dan dicontoh Rasul Paulus dalam pelayanan dan kehidupannya yang dalam 2 Timotius 1:2 dipercayakan dan diwariskan kepada Timotius untuk diteruskan kepada generasi murid-murid Yesus terus sampai kesudahan akhir zaman sungguh terbukti ampuh. Metode dalam penelitian ini menggunakan metode kualitatif dengan pendekatan studi pustaka melalui buku-buku dan artikel-artikel yang berkualitas dalam membahas pemuridan Kristen. Hasil dari penelitian ini disimpulkan bahwa implikasi kehidupan yang bermakna dari seorang murid Kristus yang memuridkan lagi yang harus nyata diperagakan dalam kehidupan kekristenannya sebagai pribadi dan gereja sebagai kehidupan komunitas murid-murid Yesus, yaitu: Pertama, Tetap tinggal di dalam Kristus. Kedua, Hidup dengan tujuan menghasilkan buah. Ketiga, Memiliki hasrat untuk regenerasi dan pelipatgandaan murid. Keempat, Meneladani Yesus yang memuridkan murid. Kelima, Gereja dan pemimpin rohani yang memuridkan. Keenam, Menjadikan pemuridan sebagai gaya hidup