Insecticidal Activity of Lichens against the Maize Weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae

Extracts of Letharia vulpina (L.) Hue and Peltigera rufescens (Weiss) Humb. lichen species and two major lichen compounds (diffractaic and usnic acids), isolated from Usnea longissima Ach., were tested against adults of the maize weevil, Sitophilus zeamais Motschulsky under laboratory conditions at various concentrations (2.5, 5, 10, 20 mg.ml-1 for extracts and 1.25, 2.5, 5, 10 mg.ml-1 for major compounds) and at 24, 48, 72, and 96 h period. Results showed that both the extracts and the secondary metabolites of U. longissima had significant insecticidal effects on adults of S. zeamais. Mortality rate was the highest at 96 h period at the treatment of maximum concentration of extracts (20 mg.ml -1) and compounds (10 mg.ml-1). The mortality rates attained 96.97, 95.96, 96.97 and 76.77% for L. vulpine, P. rufescens, diffractaic and usnic acids, respectively. No mortality was found in the control. The present results suggest that the lichen extracts and the secondary metabolites can provide a good potential for the control of S. zeamais adults

Insecticidal Effect of the Extracts of Cladonia foliacea (Huds.) Willd. and Flavoparmelia caperata (L.) Hale AgainstAdults of the Grain Weevil, Sitophilus granarius (L.) (Coleoptera: Curculionidae)

Four different concentrations of extracts, obtained from the two lichen species (Cladonia foliacea (Huds.) Willd. and Flavoparmelia caperata (L.) Hale), were tested against the grain weevil, Sitophilus granaries (L.) under laboratory conditions. Mortality rate of adults was determined at 24, 48, and 96 h. Obtained results showed that the extracts of C. foliacea and F. caperata had insecticidal effects on the adults of S. granarius in comparison with control. The insecticidal effect was influenced by the concentration of the extract and the exposure time. Higher concentrations and longer exposure time resulted to highest toxicity levels on S. granarius. Mortality rates after 96 h of treatment with highest concentration (20 mg.ml-1) of the extracts of F. caperata and C. foliacea were determined as 91 and 83%, respectively. Bioassay revealed that 96 h LC50 values were 0.107 and 0.354 mg.ml-1, respectively. The results suggest that lichen compounds could be useful as alternatives of pesticides

Multidisciplinary Treatment of an Untreated Young Adult Patient with Unilateral Complete Cleft Lip and Palate

We present the multidisciplinary treatment of a young adult patient with unilateral complete cleft lip and palate (UCLP). The patient with UCLP was 17 years old and had not applied for treatment before. He presented with a concave profile, lateral crossbite and a tete-a-tete overbite. After initial orthodontic treatment the patient’s cleft lip and nose and afterwards his palate were operated on. During the orthodontic treatment the patient had a negative overjet of 6 mm, a residuel fistule in soft palate, maxillary and secondary nose base deficiency, also a severe alveolar cleft in the premaxilla. To fix these problems, the patient’s maxilla was advanced by applying a Lefort-1 osteotomy, the secondary fistule in the soft palate was operated on and the alveolar defect was grafted with a biocollagen membrane, cansellous block graft and cansellous granular graft. The orthodontic treatment lasted 1 year following the orthognathic surgery. At the end of the orthodontic treatment Class I molar relationship was achieved on the right side and full Class II on the left side and also a 1 mm overjet and overbite. After a period of retention of 1.5 years some relapse occurred and delayed prosthetic treatment was performed by applying an adhesive bridge. Late term multidisciplinary treatment gave the UCLP patient a good appearance as well as psychological and social benefits

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Relationship between heart rate variability and radial artery spasm in patients undergoing percutaneous coronary angiography via radial access

OBJECTIVE: The objective of this study is to investigate the relationship and predictive power between heart rate variability (HRV) and radial artery spasm, in cases where the radial artery is preferred as the access route for coronary angiography (CAG). PATIENTS AND METHODS: A total of 394 patients, who were scheduled to have CAG were included in this study. Patients who developed radial artery spasms during CAG, performed using the radial artery as the access route, were analyzed for HRV parameters. RESULTS: Ages of the patients ranged between 31 and 74 years. Significant time domain measurements such as the standard deviation of normal-normal (NN) intervals, the standard deviation of the averages of NN, the average of the standard deviations of all NN intervals, and root mean square of successive differences between normal heartbeats were statistically significantly lower in the patient group that developed radial artery spasm. Frequency field measurements with prognostic values such as HF (high frequency) and very low frequency were also statistically significantly lower in the patient group that developed radial artery spasms. On the other hand, there was no statistical difference between the groups in LF (low frequency) and LF/HF ratio measurements. A statistically significantly higher radial artery spasm rate was observed in the coexistence of anxiety and low HRV. CONCLUSIONS: A significant decrease was found in major HRV values, which are closely related to the autonomic nervous system and its dysfunction, in patients with radial artery spasms

Toxicity effects of some lichen species extracts against the Colorado potato beetle, leptinotarsa decemlineata say (coleoptera: chrysomelidae)

WOS:000331597600002Extracts of the three lichen species; Lecanora muralis (Schreb.) Rabenh., Letharia vulpina (L.) Hue and Peltigera rufescens (Weiss) Humb. were tested against the 4th larval instar and adults of the Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Results showed that the three extracts had insecticidal effects on larvae and adults of the pest. Treatment with the extracts of L. vulpina and P. rufescens led to highest percentages of mortality (reached 100%). The extract of L. muralis caused the least mortality rate. Mortality rates, 120 hrs post treatment with the highest extract concentration (20 mg/ml) of L. vulpina, P. rufescens and L. muralis, were estimated as 100, 100 and 76.66% for the 4th larval instar and 100, 100 and 63.33% for adults of L. decemlineata, respectively, compared to no mortality in the control

Physiological aspect of apoptosis-regulating microRNAs expressions during fasting

OBJECTIVE: Fasting is an activi-ty that requires a certain calorie restriction with-out consuming food or drinks for a certain pe-riod of daytime. However, fasting triggers ma-ny complex events, including activating cellular stress response pathways, autophagy promo-tion, apoptosis pathways, and a change in hor-monal balance. Among the many events affect-ing the regulation of apoptosis, the expression of microRNAs (miRNAs) plays an important role. Therefore, we aimed to investigate the levels and importance of miRNA expression in fasting.PATIENTS AND METHODS: The expressions of 19 miRNAs regulating different pathways from saliva samples, isolated by matching healthy university students (n = 34) as group 1 (fasting for 17 consecutive hours) and group 2 (testing 70 minutes after meal consumption), were exam-ined using the real-time PCR method. RESULTS: In fasting, modulation of apoptotic pathways by miRNAs triggers anti-pathogenic ef-fects, and the adaptation of abnormal cells in the body decreases. For this reason, vital diseases, such as cancer, can be treated by preventing the proliferation and growth of cancerous cells by in-creasing programmed cell death due to the down -regulation expression mechanism of miRNAs.CONCLUSIONS: Our study aims to improve the knowledge about the mechanisms and func-tions of miRNAs in various apoptosis pathways during fasting and may be a model for further fu-ture physiological and pathological studies