Identification of cuproptosis-related biomarkers and analysis of immune infiltration in allograft lung ischemia-reperfusion injury

Background: Allograft lung ischemia-reperfusion injury (ALIRI) is a major cause of early primary graft dysfunction and poor long-term survival after lung transplantation (LTx); however, its pathogenesis has not been fully elucidated. Cell death is a mechanism underlying ALIRI. Cuproptosis is a recently discovered form of programmed cell death. To date, no studies have been conducted on the mechanisms by which cuproptosis-related genes (CRGs) regulate ALIRI. Therefore, we explored the potential biomarkers related to cuproptosis to provide new insights into the treatment of ALIRI.Materials and methods: Datasets containing pre- and post-LTx lung biopsy samples and CRGs were obtained from the GEO database and previous studies. We identified differentially expressed CRGs (DE-CRGs) and performed functional analyses. Biomarker genes were selected using three machine learning algorithms. The ROC curve and logistic regression model (LRM) of these biomarkers were constructed. CIBERSORT was used to calculate the number of infiltrating immune cells pre- and post-LTx, and the correlation between these biomarkers and immune cells was analyzed. A competing endogenous RNA network was constructed using these biomarkers. Finally, the biomarkers were verified in a validation set and a rat LTx model using qRT-PCR and Western blotting.Results: Fifteen DE-CRGs were identified. GO analysis revealed that DE-CRGs were significantly enriched in the mitochondrial acetyl-CoA biosynthetic process from pyruvate, protein lipoylation, the tricarboxylic acid (TCA) cycle, and copper-transporting ATPase activity. KEGG enrichment analysis showed that the DE-CRGs were mainly enriched in metabolic pathways, carbon metabolism, and the TCA cycle. NFE2L2, NLRP3, LIPT1, and MTF1 were identified as potential biomarker genes. The AUC of the ROC curve for each biomarker was greater than 0.8, and the LRM provided an excellent classifier with an AUC of 0.96. These biomarkers were validated in another dataset and a rat LTx model, which exhibited good performance. In the CIBERSORT analysis, differentially expressed immune cells were identified, and the biomarkers were associated with the immune cells.Conclusion:NFE2L2, NLRP3, LIPT1, and MTF1 may serve as predictors of cuproptosis and play an important role in the pathogenesis of cuproptosis in ALIRI

Highly flexible, electrically driven, top-emitting, quantum dot light-emitting stickers

Flexible information displays are key elements in future optoelectronic devices. Quantum dot light-emitting diodes (QLEDs) with advantages in color quality, stability, and cost-effectiveness are emerging as a candidate for single-material, full color light sources. Despite the recent advances in QLED technology, making high-performance flexible QLEDs still remains a big challenge due to limited choices of proper materials and device architectures as well as poor mechanical stability. Here, we show highly efficient, large-area QLED tapes emitting in red, green, and blue (RGB) colors with top-emitting design and polyimide tapes as flexible substrates. The brightness and quantum efficiency are 20 000 cd/m2 and 4.03%, respectively, the highest values reported for flexible QLEDs. Besides the excellent electroluminescence performance, these QLED films are highly flexible and mechanically robust to use as electrically driven light-emitting stickers by placing on or removing from any curved surface, facilitating versatile LED applications. Our QLED tapes present a step toward practical quantum dot based platforms for high-performance flexible displays and solid-state lighting. © 2014 American Chemical Society

Development and Application of a Crossed Multi-Arch Greenhouse in Tropical China

Deep analysis and demonstration of the developed crossed multi-arch greenhouse were conducted from the perspectives of conceptual design, architectural and structural design, functional design, loading parameters, and structural internal forces. The results show that the crossed multi-arch greenhouse combines the ventilation area between the floor-standing round-arch greenhouse and the unsuitable operation area under the arch bars into one to form a multi-span crossed arch structure with good ventilation and heat dissipation, land savings, and fine mechanical behaviors. The main arch structure uses 32.4% less steel and 25% less foundation volume than the control greenhouse under the same load, which can save about CNY 10,184.00/667m2 of investment according to the current cost level. In the meantime, ventilation simulation analysis of the developed crossed multi-arch greenhouse was carried out using the software Design Builder. A comparison shows that, under the condition of no wind and breeze (1 m/s) in summer, the setting of the ventilation channel has obvious advantages for the heat dissipation of the greenhouse, and the average temperature is about 2 °C lower than that of the greenhouse without a ventilation channel; under the breeze condition, the temperature in the crossed multi-arch greenhouse is more evenly distributed than that of an ordinary round-arch greenhouse with ventilation channels. Considering the greenhouse function, building cost, using effect, and other evaluation factors, the crossed multi-arch greenhouse can meet the production environment requirements of tropical coastal areas (rain protection, sunshade, and ventilation), with obvious structural advantages, good typhoon resistance, and low construction costs, which is a preferable choice of greenhouse type

High-efficiency all-inorganic full-colour quantum dot light-emitting diodes

All-inorganic quantum dot light-emitting diodes (QLEDs) with excellent device stability have attracted significant attention for solid state lighting and flat panel display applications. However, the performance for the present all-inorganic QLEDs is far inferior to that of the well-developed QLEDs with organic charge transport layers. Our all-inorganic full-colour QLEDs show the maximum brightness and efficiency values of 21,600 cd/m2 and 6.52%, respectively, which are record-breaking among the existing all-inorganic QLEDs. The outstanding performance is achieved by an efficient design of device architecture with solution-processed charge transport layers (CTLs). Meanwhile, the ultrathin double-sided insulating layers are inserted between the quantum dot emissive layer and their adjacent oxide electron transport layers to better balance charge injection in the device and reduce the quenching effects for inorganic CTLs on QD emission. This study is the first account for high-performance, all-inorganic QLEDs insightfully offering detailed investigations into the performance promotion for inorganic electroluminescent devices

3D Photoluminescent Nanostructures Containing Quantum Dots Fabricated by Two-Photon Polymerization: Influence of Quantum Dots on the Spatial Resolution of Laser Writing

International audienceQuantum dots (QDs) films or layers are extensively used for various photonic and electronic applications since recent decades. Three‐dimensional (3D) nanostructures are the elegant fashion in micro‐optics, microfluidics, biomedicine, and imaging. In particular, 3D photoluminescent nanostructures play an important role in detection and sensing as well as emitting systems. In this work, 3D photoluminescent structures containing QDs with feature sizes down to 80 nm are fabricated via two‐photon polymerization‐based direct laser writing. The photoluminescent images and emission spectra show strong local emission of blue, green, and red light from the polymer micro and nanostructures. The influence of the QDs, laser power, and writing scan speed on the resolution of the as‐prepared structures is also investigated. It turns out that the presence of QDs increases the lateral resolution where the feature size of polymer lines can reach 75 nm in 3D. This promising result paves the way for the integration of single nano‐emitters with a fine control of their spatial positioning in some nanophotonic devices

Effects of fabrics with dynamic moisture transfer properties on skin temperature in females during exercise and recovery

Based on the physiological nature of breast movement in exercising females, a sports bra made of fabric with dynamic moisture transfer properties was developed to improve female thermal comfort. This study aimed to investigate the effects of fabrics with dynamic moisture transfer properties on breast skin temperature, and the thermal physiological and psychological response of women while wearing the sports bra during exercise and recovery. Ten healthy women exercised in random order with two types of sports bra with or without the dynamic moisture transfer properties and then performed a 20-minute short-duration high-intensity exercise and rest to recover under thermoneutral conditions. Heart rate, body core temperature, skin temperature, body mass and thermal psychological subjective sensations were investigated during exercise and recovery. The results indicated that in the running state, the local breast skin temperatures of sports bra made of fabrics with dynamic moisture transfer properties (33.427±0.087) are significantly lower than bras without these dynamic moisture transfer properties (33.964±0.055) (P<0.01). During the exercise and recovery, the thermal psychological subjective sensation for the two types of fabrics were very similar, whereas the body mean skin temperature was revealed to undergo greater decreasing effects in sports bras made of fabrics with dynamic moisture transfer properties than those without the dynamic moisture transfer properties (P<0.05). These results provide novel information that usage of fabrics with dynamic moisture properties in sports bras could improve thermoregulation to benefit exercising women’s thermal comfort in terms of decreasing local breast skin temperature.Institute of Textiles and Clothin