Identification of Conserved miRNAs in Solanum Lycopersicum Response to Long-term RPM-treatment

Abstract-T o identify the miRNAs associated with the simulated microgravity response in plants and to ascertain the regulation network mediated by miRNAs under simulated microgravity conditions, we constructed a miRNA library by direct cloning method and analyzed the library. Six conserved Solanum lycopersicum miRNAs were identified for the first time in Solanum lycopersicum under simulated microgravity condition. Gene ontology analysis showed that most of the predicted targeted genes were involved in organelle part, transcription factor, signal transduction and metabolic process, implying a complicated relationship among the external signal, internal transduction and final phenotype. Cis-elements located in the upstream sequences of each miRNA were identified and their roles in gene regulation were investigated. In the study, miRNAs were identified in S. lycopersicum for the first time under long-term simulated microgravity condition, which will help reveal the regulation mechanism mediated by miRNAs under simulated microgravity condition and adaptation to Earth's gravity

Geology, U-Pb geochronology and stable isotope geochemistry of the Heihaibei gold deposit in the southern part of the Eastern Kunlun Orogenic Belt, China : A granitic intrusion-related gold deposit?

The Heihaibei gold deposit is a newly discovered gold deposit in the southern part of the Eastern Kunlun Orogenic Belt. Its most distinctive features are that the gold mineralization is hosted in monzogranite, and that the presence of pre-ore (possibly syn-ore) monzogranite and post-ore gabbro allows to constrain the minerali-zation's formation age. Zircons from the monzogranites yield U-Pb ages of 454 +/- 3 Ma, while zircons separated from the gabbro dikes cutting the monzogranites and gold mineralized body yield U-Pb ages of 439 +/- 3 Ma, which is interpreted to be the minimum age of the Au mineralizing event. Combined with the regional geological background, we proposed that the Heihaibei Au mineralization occurred during the subduction stage of the Early Paleozoic Proto-Tethys ocean. The ore assemblage is dominated by pyrite, arsenopyrite and native gold. The hydrothermal alteration that has led to the peculiar enrichment of Au is not systematically distributed and displays no clear concentric zoning pattern. The main mineralization formed during three stages: the K-feldspar-quartz-pyrite (Py1)-arsenopyrite-sericite-epidote stage (I), the quartz-pyrite (Py2)-native gold-chlorite stage (II), and the quartz-carbonate stage (III). The main gold mineralization occurred during stage II. Fluid inclusion homogenization temperature and salinities decrease from stage I (Th., 268-412 C; W., 6.87-16.63 wt% NaCl equiv.) to stage II (Th., 183-288 C; W., 3.69-14.84 wt% NaCl equiv.). The 818O and 8D values (818OH2O = 4.9 to 9.7%o; 8DV-SMOW =-84.1%o to -81.1%o) of quartz samples from stage I and stage II are comparable to a magmatic-hydrothermal ore-forming fluid that possibly underwent fluid-rock interaction with the Nachitai Group metamorphic rocks during the early ore-forming stage. The relatively uniform 834S values (834SV-CDT = 7.7 to 8.5%o) are slightly elevated compared to magmatic 834S values, but could be derived from a magma if a significant crustal melt component is present. Moreover, the 834S values are within the S isotopic composition range of a granitic reservoir, suggesting that they are probably inherited from the Heihaibei monzogranites. The Pb and Hf isotope compositions imply a close genetic association between the gold mineralization and granitic magmatism, which are both the products of the mixing of crustal and mantle sources. The trace element compositions of pyrite provide additional evidence that the gold mineralization in the Heihaibei deposit was related to the magmatism. Compared with the typical characteristics of orogenic gold and intrusion-related gold systems (IRGS) deposits, the Heihaibei gold deposit may instead be classified as a granitic intrusion-related gold deposit.Peer reviewe

Multi-Energy Load Collaborative Optimization of the Active Building Energy Management Strategy

Under the dual-carbon target, the popularization and application of building integrated photovoltaic (BIPV) and ground source heat pump systems have made active buildings a research hotspot in the field of architecture and energy. Aiming at this issue, based on the building energy consumption model of active buildings, an active building energy management system (EMS) control strategy based on multi-energy load collaborative optimization is proposed. Firstly, based on the thermal dynamic characteristics and building performance parameters of active buildings, the overall refined energy consumption model of active buildings is constructed. Secondly, based on the construction of BIPV, the ice storage air conditioning system, the ground source heat pump system, and the integrated demand response (IDR) model, a tiered carbon transaction cost model is introduced, and an energy management strategy that leverages the synergistic application of renewable and active technologies is proposed. This strategy aims to meet the comprehensive needs of active buildings in terms of economic benefits, comfort, and environmental protection. Finally, the strategy’s effectiveness is demonstrated through a practical example

Differences in Career Decision-Making Profiles Between American and Chinese University Students

The current research examined differences in career decision-making profiles (CDMP) between American and Chinese university students, as well as the mediating mechanisms possibly underlying these cultural differences. The results of a survey among American (n = 929) and Chinese (n = 945) undergraduates showed that Chinese participants scored significantly higher on consulting with others, desire to please others, willingness to compromise, dependence on others, and procrastination, but lower on aspiration for an ideal occupation, internal locus of control, and effort invested in career decision-making than did the American participants. Using a model based on self-construals and subjective cultural norms, we established that interdependent self-construal, independent self-construal, and the perceived individualism-collectivism norm operative in the respondents’ nation served as important mediators of the relationship between culture and endorsement of the dimensions of the CDMP. Moreover, based on the model of cultural tightness-looseness, the results provided partial support for the prediction that individuals’ personal cultural orientations (e.g., self-construals) served as stronger predictors for CDMP among the American participants than among the Chinese, whereas the perceived cultural norm served as a stronger predictor for CDMP among the Chinese participants than the Americans. The current research provides implications for career decision-making in different cultural groups and suggests the operation of differential mechanisms involved in reaching career decisions across societies varying in individualism-collectivism.Department of Management and MarketingDepartment of Applied Social Science

A metagenome-wide association study of gut microbiota in type 2 diabetes

Assessment and characterization of gut microbiota has become a major research area in human disease, including type 2 diabetes, the most prevalent endocrine disease worldwide. To carry out analysis on gut microbial content in patients with type 2 diabetes, we developed a protocol for a metagenome-wide association study (MGWAS) and undertook a two-stage MGWAS based on deep shotgun sequencing of the gut microbial DNA from 345 Chinese individuals. We identified and validated approximately 60,000 type-2-diabetes-associated markers and established the concept of a metagenomic linkage group, enabling taxonomic species-level analyses. MGWAS analysis showed that patients with type 2 diabetes were characterized by a moderate degree of gut microbial dysbiosis, a decrease in the abundance of some universal butyrate-producing bacteria and an increase in various opportunistic pathogens, as well as an enrichment of other microbial functions conferring sulphate reduction and oxidative stress resistance. An analysis of 23 additional individuals demonstrated that these gut microbial markers might be useful for classifying type 2 diabetes

A metagenome-wide association study of gut microbiota in type 2 diabetes

Assessment and characterization of gut microbiota has become a major research area in human disease, including type 2 diabetes, the most prevalent endocrine disease worldwide. To carry out analysis on gut microbial content in patients with type 2 diabetes, we developed a protocol for a metagenome-wide association study (MGWAS) and undertook a two-stage MGWAS based on deep shotgun sequencing of the gut microbial DNA from 345 Chinese individuals. We identified and validated approximately 60,000 type-2-diabetes-associated markers and established the concept of a metagenomic linkage group, enabling taxonomic species-level analyses. MGWAS analysis showed that patients with type 2 diabetes were characterized by a moderate degree of gut microbial dysbiosis, a decrease in the abundance of some universal butyrate-producing bacteria and an increase in various opportunistic pathogens, as well as an enrichment of other microbial functions conferring sulphate reduction and oxidative stress resistance. An analysis of 23 additional individuals demonstrated that these gut microbial markers might be useful for classifying type 2 diabetes