Bioinformatics-based analysis of the roles of basement membrane-related gene AGRN in systemic lupus erythematosus and pan-cancer development

IntroductionSystemic lupus erythematosus (SLE) is an autoimmune disease involving many systems and organs, and individuals with SLE exhibit unique cancer risk characteristics. The significance of the basement membrane (BM) in the occurrence and progression of human autoimmune diseases and tumors has been established through research. However, the roles of BM-related genes and their protein expression mechanisms in the pathogenesis of SLE and pan-cancer development has not been elucidated.MethodsIn this study, we applied bioinformatics methods to perform differential expression analysis of BM-related genes in datasets from SLE patients. We utilized LASSO logistic regression, SVM-RFE, and RandomForest to screen for feature genes and construct a diagnosis model for SLE. In order to attain a comprehensive comprehension of the biological functionalities of the feature genes, we conducted GSEA analysis, ROC analysis, and computed levels of immune cell infiltration. Finally, we sourced pan-cancer expression profiles from the TCGA and GTEx databases and performed pan-cancer analysis.ResultsWe screened six feature genes (AGRN, PHF13, SPOCK2, TGFBI, COL4A3, and COLQ) to construct an SLE diagnostic model. Immune infiltration analysis showed a significant correlation between AGRN and immune cell functions such as parainflammation and type I IFN response. After further gene expression validation, we finally selected AGRN for pan-cancer analysis. The results showed that AGRN’s expression level varied according to distinct tumor types and was closely correlated with some tumor patients’ prognosis, immune cell infiltration, and other indicators.DiscussionIn conclusion, BM-related genes play a pivotal role in the pathogenesis of SLE, and AGRN shows immense promise as a target in SLE and the progression of multiple tumors

The relationship between the interactive behavior of industry–university–research subjects and the cooperative innovation performance: The mediating role of knowledge absorptive capacity

IntroductionIndustry–university–research cooperation innovation, which is often characterized by resource complementarity and the sharing technology, has become one of the most preferred innovation cooperation methods for enterprises. However, various problems still occur in the process of industry–university–research cooperations, such as poor innovation performance and difficulty in sustaining cooperation. Existing studies mostly focus on the macroscopic perspectives of geographic location, cooperation scale, concentration, and diversification of industry–university–research cooperation subjects, and fail to explore the microscopic behavioral mechanisms.MethodsTherefore, this paper establishes the interactive behavior of industry–university–research subjects and defines its concepts and dimensions in an attempt to provide a mechanism for improving the cooperative innovation performance of industry–university–research from the micro-behavioral perspective. On the basis of theoretical analysis, this paper develops a model of the relationship between cooperative trust, cooperative communication, and cooperative innovation performance for interactive behavior, while exploring the mediating role of knowledge absorptive capacity. The model was validated by stepwise regression using data from 325 questionnaires.ResultsThe paper found that cooperative trust and cooperative communication in the cooperative interactive behavior of industry–university–research positively contribute to the improvement of cooperative innovation performance. Knowledge absorptive capacity plays a partially mediating role between the interactive behaviors and cooperative innovation performance. More specifically, knowledge absorptive capacity partially mediates cooperative communication in cooperative innovation performance and completely mediates cooperative trust in cooperative innovation performance. The results are largely consistent with the results of the heterogeneity analysis of the sample.DiscussionThis paper not only explains why the cooperative innovation performance of industry–university–research is poor from the perspective of interactive behavior, but also enriches the research perspective of industry–university–research and provides theoretical support for enterprises to optimize the relationship between industry, university, and research institutes

Genome-wide identification of the AcMADS-box family and functional validation of AcMADS32 involved in carotenoid biosynthesis in Actinidia

MADS-box is a large transcription factor family in plants and plays a crucial role in various plant developmental processes; however, it has not been systematically analyzed in kiwifruit. In the present study, 74 AcMADS genes were identified in the Red5 kiwifruit genome, including 17 type-I and 57 type-II members according to the conserved domains. The AcMADS genes were randomly distributed across 25 chromosomes and were predicted to be mostly located in the nucleus. A total of 33 fragmental duplications were detected in the AcMADS genes, which might be the main force driving the family expansion. Many hormone-associated cis-acting elements were detected in the promoter region. Expression profile analysis showed that AcMADS members had tissue specificity and different responses to dark, low temperature, drought, and salt stress. Two genes in the AG group, AcMADS32 and AcMADS48, had high expression levels during fruit development, and the role of AcMADS32 was further verified by stable overexpression in kiwifruit seedlings. The content of α-carotene and the ratio of zeaxanthin/β-carotene was increased in transgenic kiwifruit seedlings, and the expression level of AcBCH1/2 was significantly increased, suggesting that AcMADS32 plays an important role in regulating carotenoid accumulation. These results have enriched our understanding of the MADS-box gene family and laid a foundation for further research of the functions of its members during kiwifruit development

Perbandingan Perhitungan Trafik Jam Sibuk CDMA 2000 1x Pada BTS Inner City Dan BTS Outer City Dengan Mempergunakan Metode ADPH, TCBH, FDMH Dan FDMP

Cellular communication system is a wireless communication system where the subscriber can move within a wide network coverage. Code Division Multiple Access (CDMA) is a multiuser access technology that is each user uses a unique code contained in the access channel in the system. Calculation and determination of peak hours can be done by several methods such as: Average Daily Peak Hour (ADPH), Time Consistent Busy Hour (TCBH), Fixed Daily Measurement Hour (FDMH), Fixed Daily Measurement Period (FDMP). The effectiveness of the channel should be determined by occupancy both at inner city territory and outer city  territory location. Using design Erlang (Erl) for supply channel at Base Transceiver Station (BTS) that provided, BTS has a design Erlang of 369,83 Erl at inner city and it has a design Erlang of 241,8 Erl at outer city. Peak hour on the inner city occurred at 12:00 to 15:00, whereas the outer city of peak hour occurred at 18:00 to 21:00. Effectiveness value that determined by operator are : <20% = low occupancy (not effective), 21% to 69% = normal occupancy (effective), and > 70% = high occupancy (very effective). In this case occupancy values obtained in each method is between 21% to 69% which means effectiv

Addressing Antimicrobial Resistance in China: Policy Implementation in a Complex Context

The effectiveness of antibiotics in treating bacterial infections is decreasing in China because of the widespread development of resistant organisms. Although China has enacted a number of regulations to address this problem, but the impact is very limited. This paper investigates the implementation of these regulations through the lens of complex adaptive systems (CAS). It presents the findings from reviews of relevant policy documents and published papers. The paper identifies different types of agent and explores their interaction with regard to the use of antibiotics and their responses to changes of the regulations. It focuses particularly on the impact of perverse financial incentives on overall patterns of use of antibiotics. Implications for the possibilities of nonlinear results, interactive relationships, and new pathways of policy implementation are discussed. The paper concludes that policy-makers need to better understand the objectives, incentives and potential adaptive behaviors of the agents when they implement interventions to improve antibiotic use and reduce the risk of emergence of resistant organism

Effect of Formulated Fertilization on Fruit Quality of Early Ripe Peach

Using the early maturing peach variety 'Japan Matsusen' as the test material, nine groups of formula fertilization schemes were set up by orthogonal experiment to study the effects of different fertilization treatments on the quality of peach fruit, in order to obtain the best fertilization formula to improve fruit quality. The results showed that the quality of peach fruit was significantly improved by adding organic fertilizer to the base fertilizer instead of chemical fertilizer. Therefore, by adding organic fertilizer as the base fertilizer, reducing the application amount of chemical fertilizer, and adding other trace element fertilizers can effectively improve the quality of peach fruit

Exogenous Melatonin Application Delays Senescence of Kiwifruit Leaves by Regulating the Antioxidant Capacity and Biosynthesis of Flavonoids

Melatonin, a multiple signal molecule, plays important roles in delaying senescence during the development of plants. Because few species have been studied for the effect of exogenous melatonin on anti-aging, the plausible mechanism of melatonin of anti-aging effects on other plant species has remained largely unknown. In the present study, the effects of exogenous melatonin on leaf senescence in kiwifruit were examined during natural aging after melatonin (200 μM) or water (Control) pretreatment. The decreased membrane damage and lower hydrogen peroxide (H2O2) content due to the enhanced scavenging activity of antioxidant enzymes peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) demonstrated that melatonin effectively delayed the aging of kiwifruit leaves. Likewise, owing to up-regulated expression of chlorophyll a/b-binding protein (CAB) gene in the sampled leaves pretreated with melatonin, chlorophyll degradation decreased. Therefore, osmoregulatory substances in sampled leaves accumulated (e.g., soluble sugar and soluble protein) and seedling cell environment stability was maintained. Simultaneously, melatonin decreased H2O2 concentration owing to increased glutathione (GSH) and ascorbate (AsA) content, and the expression levels of glutathione reductase (GR), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR) were up-regulated by melatonin application, indicating that the increase of GSH and AsA was attributed to the expression of these genes. In addition, a large amount of flavonoids accumulated in seedlings pretreated with melatonin, and transcript levels of eight genes involved in flavonoid synthesis, including phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxymate (C4H), chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), flavonol synthase (FNS), leucoanthocyanin reductase (LAR), anthocyanin reductase (ANR), flavonoid 3-O-glucosyltransferase (UFGT) were enhanced in response to melatonin application. These results indicated that melatonin delayed aging of kiwifruit leaves by activating the antioxidant capacity and enhancing flavonoid biosynthesis. All of these results can provide clear proof that melatonin plays a key roles in delaying leaf senescence

Effects of different ways of flower bud differentiation on bud germination and fruit quality of grape

In this study, 3 year-old ‘Xiahei’ grape was used as the test material. It aimed to promote the differentiation of grape flower buds with different concentrations of paclobutrazol and chlormequat chloride, and the effects of different ways of promoting the differentiation of flower buds on the germination of grape buds and fruit quality were studied. The results showed that different ways of flower bud differentiation could promote the germination of ‘Xiahei’ grape buds. In consideration of the fruiting habit, phenological period and fruit quality of different treatments, second fruits which were sprayed with 1000 times 15% paclobutrazolin had the best effect. On May 1, 2019 (One fruit sitting period) spraying grape new tip with 1000 times 15% paclobutrazol and 0.3% potassium dihydrogen phosphate, 0.1% boric fertilizer and the same fungicides, spraying again after 7 days, reagent type and concentration were same as the first time, after 10 days of the second spraying 2.5% hydrogen cyanamide was used to break dormancy of winter bud. The way could effectively promote grape secondary fruit and improve the quality of the grapes