Do lncRNAs and circRNAs expression profiles influence discoid lupus erythematosus progression?—a comprehensive analysis.

Background: Long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs)are involved in the progression of discoid lupus erythematosus (DLE), but an understanding of their underlying mechanisms remains elusive. To explore the expression profiles of lncRNAs and circRNAs in DLE, we surveyed the lncRNA/circRNA and mRNA expression profiles in the epithelia of oral DLE and adjacent normal tissues. Methods: The lesional and non-lesional lower lips of three DLE patients were analysed by RNAsequencing (RNA-seq). The principal functions of the significantly deregulated genes were identified using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. And the correlated expression networks (coding-noncoding co-expression and lncRNAstranscription factor-mRNA) were evaluated as well. Results: Hundreds of significantly changed lncRNAs and mRNAs and dozens of significantly changed circRNAs were identified. lncRNA lnc-MIPOL1-6 and IncRNA IncDDX47-3 expressions were correlated with immune response-related genes, including IL19, CXCL1, CXCL11, and TNFSF15. Up-regulated IncRNA-TF network consists of 8 TFs and 74 related lncRNAs. The lncRNA-TF-gene trans-regulation consisting of 204 lncRNAs,39 TFs, and correlated 3 genes. Conclusions: These results demonstrate that lncRNAs and circRNAs can influence the progression of DLE. Certain mRNAs/lncRNAs/circRNAs may have substantial value in DLE diagnosis and therapy

Lowest-order QED radiative corrections in unpolarized elastic electron-deuteron scattering beyond the ultra-relativistic limit for the proposed deuteron charge radius measurement at Jefferson Laboratory

Analogous to the well-known proton charge radius puzzle, a similar puzzle exists for the deuteron charge radius, $r_{d}$. There are discrepancies observed in the results of $r_{d}$, measured from electron-deuteron ($e-d$) scattering experiments, as well as from atomic spectroscopy. In order to help resolve the charge radius puzzle of the deuteron, the PRad collaboration at Jefferson Lab has proposed an experiment for measuring $r_{d}$, named DRad. This experiment is designed to measure the unpolarized elastic $e-d$ scattering cross section in a low-$Q^{2}$ region. To extract the cross section with a high precision, having reliable knowledge of QED radiative corrections is important. In this paper, we present complete numerical calculations of the lowest-order radiative corrections in $e-d$ scattering for the DRad kinematics. The calculations have been performed within a covariant formalism and beyond the ultra-relativistic approximation ($m_{e}^{2} \ll Q^{2}$). Besides, we present a systematic uncertainty on $r_{d}$ arising from higher-order radiative corrections, estimated based on our cross-section results.Comment: 16 pages and 6 figure

Photosynthesis-independent production of reactive oxygen species in the rice bundle sheath during high light is mediated by NADPH oxidase.

When exposed to high light, plants produce reactive oxygen species (ROS). In Arabidopsis thaliana, local stress such as excess heat or light initiates a systemic ROS wave in phloem and xylem cells dependent on NADPH oxidase/respiratory burst oxidase homolog (RBOH) proteins. In the case of excess light, although the initial local accumulation of ROS preferentially takes place in bundle-sheath strands, little is known about how this response takes place. Using rice and the ROS probes diaminobenzidine and 2',7'-dichlorodihydrofluorescein diacetate, we found that, after exposure to high light, ROS were produced more rapidly in bundle-sheath strands than mesophyll cells. This response was not affected either by CO2 supply or photorespiration. Consistent with these findings, deep sequencing of messenger RNA (mRNA) isolated from mesophyll or bundle-sheath strands indicated balanced accumulation of transcripts encoding all major components of the photosynthetic apparatus. However, transcripts encoding several isoforms of the superoxide/H2O2-producing enzyme NADPH oxidase were more abundant in bundle-sheath strands than mesophyll cells. ROS production in bundle-sheath strands was decreased in mutant alleles of the bundle-sheath strand preferential isoform of OsRBOHA and increased when it was overexpressed. Despite the plethora of pathways able to generate ROS in response to excess light, NADPH oxidase-mediated accumulation of ROS in the rice bundle-sheath strand was detected in etiolated leaves lacking chlorophyll. We conclude that photosynthesis is not necessary for the local ROS response to high light but is in part mediated by NADPH oxidase activity