MLModelScope website development with react

With the rapid growth of the MLModelScope project, there is an urgent need for a user interface to show users the available resources, functionalities, and facilitate their experiments. The project was designed to develop a website with React for MLModelScope which entailed providing an interactive user interface to easily demonstrate its functionalities. Also, other users can use our platform to run some experiments without setting up the whole system. In this thesis, we will first give an introduction to MLModelScope and React and also the goal of this project. Then a discussion follows to describe the whole design and development process and the problems we faced in each stage. Next, we dive into the details of the technologies we used and how we dealt with some technical challenges. Finally, we presented our opinions about this project, thoughts for developing in React and plans for further development

Metabolism of Functional Oligosaccharides and Antagonism of Foodborne Pathogens by Resting Cells of Limosilactobacillus reuteri

Based on the metabolic activity and biotransformation capacity of the resting cells of Limosilactobacillus reuteri, its ability to metabolize functional oligosaccharides and antagonize foodborne pathogens was explored. A phylogenetic tree for L. reuteri was constructed based on its 16S rRNA gene sequence. The environmental tolerance of the resting cells of L. reuteri was analyzed by acid and bile salt tolerance, and antibiotic sensitivity assays. The effects of functional oligosaccharides on the metabolism, surface hydrophobicity, adhesion capacity and reuterin (a broad-spectrum antibacterial) production ability of L. reuteri were evaluated by using modified media. Then, the inhibitory effect of the resting cells of L. reuteri on the adhesion of Staphylococcus aureus to Caco-2 cells and its growth in skim milk was explored. Results showed that both L. reuteri strains tested could tolerate extreme acidic conditions and bile salts for more than 3 h and were susceptible to common antibiotics. All functional oligosaccharides tested except xylo-oligosaccharides could be metabolized by the resting cells, and its growth, surface hydrophobicity and adhesion capacity were improved significantly by raffinose (P < 0.05). Raffinose could promote the production of reuterin from L. reuteri HLRE13 by utilizing glycerol, attaining a concentration of (1.34 ± 0.03) g/L. Glycerol promoted the inhibitory effect of L. reuteri HLRE13 on a variety of foodborne pathogens significantly (P < 0.001), and inhibited the adhesion of S. aureus to Caco-2 cells by 41.67% through exclusion. Furthermore, glycerol could promote L. reuteri HLRE13 to reduce the number of S. aureus to 103 CFU/mL during co-culture. This study will provide a scientific basis for the functional development of resting cells of L. reuteri and its synergistic efficacy with functional oligosaccharides

Effect of Nicotinamide Against Candida albicans

Nicotinamide (NAM) has a long history in clinical applications and can be safely used for treating various diseases. In recent years, NAM was found to exhibit antimicrobial activities, inhibiting the growth of Plasmodium falciparum, Mycobacterium tuberculosis, and human immunodeficiency virus (HIV). Here we investigated the activity of NAM against Candida albicans, one of the most prevalent human fungal pathogens. Our results showed that NAM exhibited significant antifungal activity against C. albicans, including fluconazole-resistant isolates. NAM could also effectively suppress biofilm formation. In addition, NAM exhibited antifungal activity against non-Candida albicans species and Cryptococcus neoformans. Combination of NAM and fluconazole showed an even strong antifungal activity. The antifungal activity of NAM was further confirmed in a mouse model of disseminated candidiasis. Confocal laser scanning microscopy revealed that NAM increased cell wall β-glucans exposure and chitin content while decreased mannan level. Furthermore, by screening the C. albicans homozygous deletion mutant library, the C. albicans mutant lacking GIN4, which encodes a septin regulatory protein kinase and is essential for the maintenance of cell wall integrity, was identified to be high sensitive to NAM. These findings suggested that NAM might exhibit antifungal activities through affecting cell wall organization