Optical and electrochemical dual detection of β-lactoglobulin based on the methylene blue@copper-based metal-organic framework

β-lactoglobulin is an effective indicator of allergic protein detection. Herein, we produced a copper-based metal-organic framework coated with methylene blue, to realize the optical and electrochemical dual detection of β-lactoglobulin. Methylene blue was successfully encapsulated inside the copper-based metal-organic framework and released after addition of β-lactoglobulin. As the concentration of β-lactoglobulin increased, the intensity of the ultraviolet absorption band and the response current increased with the increasing concentration of methylene blue released from the copper-based metal-organic framework. The optical detection range is from 0.10 mg/mL to 10 mg/mL, and the detection limit is 0.10 mg/mL. The electrochemical detection range is from 1.0 × 10−7 mg/mL to 8.0 × 10−7 mg/mL, the detection limit is 2.0 × 10−8 mg/mL. The dual detection strategy, with no interfere with each other, played a synergetic role in the quick qualitative and precise quantitative analyses of β-lactoglobulin in a wide range of applications

Effect of Brazing Fillet on the Microstructure and Mechanical Properties of Vacuum Brazing Stainless Steel Joints

The enhancement mechanism of the fillet on brazing joints is of great significance for vacuum brazing technology. Although a lot of research on the enhancement mechanism of the fillet has been carried out, some key components of a comprehensive systematic enhancement mechanism for brazing fillets have yet to be established. In this paper, the enhancement mechanism for brazing fillets of SS304/pure copper brazing joints was studied by both experimental and numerical simulations. The SEM and tensile experiments were used to characterize the microstructure and shear strength of the brazing joints. The results show that the brazing joints, using 60 µm thick pure copper filler metal, exhibit a good microstructure in the brazing seam; however, its mechanical properties are lower than those found in specimens with a thickness of 90 µm. The fracture behaviors of brazing joints were also investigated, the fracture of the brazing seam was a fracture of mode II due to shear stress, while the fracture of the brazing fillet was caused by a combination of tensile stress and shear stress (mode I and mode II)

A Cruciform Petal-like (ZIF-8) with Bactericidal Activity against Foodborne Gram-Positive Bacteria for Antibacterial Food Packaging

Most antibacterial nanomaterials used in food packaging act by releasing reactive oxygen species (ROS), which cannot efficiently have an inhibitory effect by penetrating the cell wall of Gram-positive Staphylococcus aureus. In this work, we used the cruciform petal-like zeolite imidazole framework-8 (ZIF-8) synthesized in the water phase which can release active Zn compounds in aqueous solution and exert a stronger inhibitory effect on S. aureus. The experimental results demonstrated that the aqueous cruciform petal-like ZIF-8 has the same photocatalytic activity as traditional ZIF-8 and can be applied in photocatalytic bacterial inactivation. The cruciform petal-like ZIF-8 was also shown to release active Zn compounds in aqueous solution with a better antibacterial effect against S. aureus, reaching 95% inactivation efficiency. The antibacterial effect was therefore 70% higher than that of traditional ZIF-8. Based on its excellent antibacterial properties, we loaded petal-like ZIF-8, PDA and PVA onto ordinary fibers to prepare ZIF-8-Film. The results further showed that ZIF-8-Film has a high filtration capacity, which can be used in antibacterial packaging material with the required air permeability. Moreover, ZIF-8-Flim can clean the surface on its own and can maintain a sterile environment. It is different from other disposable materials on the market in that it can be reused and has a self-disinfection function

A candidate RxLR effector from Plasmopara viticola can elicit immune responses in Nicotiana benthamiana

Abstract Background Diverse plant pathogens deliver effectors into plant cells to alter host processes. Oomycete pathogen encodes a large number of putative RxLR effectors which are likely to play a role in manipulating plant defense responses. The secretome of Plasmopara viticola (downy mildew of grapevine) contains at least 162 candidate RxLR effectors discovered in our recent studies, but their roles in infection and pathogenicity remain to be determined. Here, we characterize in depth one of the putative RxLR effectors, PvRxLR16, which has been reported to induce cell death in Nicotiana benthamiana in our previous study. Results The nuclear localization, W/Y/L motifs, and a putative N-glycosylation site in C-terminal of PvRxLR16 were essential for cell death-inducing activity. Suppressor of G-two allele of Skp1 (SGT1), heat shock protein 90 (HSP90) and required for Mla12 resistance (RAR1), but not somatic embryogenesis receptor-like kinase (SERK3), were required for the cell death response triggered by PvRxLR16 in N. benthamiana. Some mitogen-activated protein kinases and transcription factors were also involved in the perception of PvRxLR16 by N. benthamiana. PvRxLR16 could also significantly enhance plant resistance to Phytophthora capsici and the nuclear localization was required for this ability. However, some other PvRxLR effectors could suppress defense responses and disease resistance induced by PvRxLR16, suggesting that it may not trigger host cell death or immune responses during physiological infection under natural conditions. Conclusion These data demonstrate that PvRxLR16 may be recognized by endogenous proteins in nucleus to trigger immune responses in N. benthamiana, which in turn can be suppressed by other PvRxLR effectors

Effect of Oily Sludge Treatment with Molten Blast Furnace Slag on the Mineral Phase Reconstruction of Water-Quenched Slag Properties

Blast furnace slag, which is the main by-product of the ironmaking process discharged at 1450 °C, contains high-quality sensible heat, while oily sludge is the main solid waste produced in the process of gas exploration, storage, and transportation. The energy and resource utilization of blast furnace slag is complementary to the environmentally friendly treatment of oily sludge, which has provided a new idea for the multi-factor synergistic cycle and energy transformation of the two wastes. The pyrolysis of the oily sludge with the molten blast furnace slag was conducted in the current paper. Results showed that the oily sludge was rapidly pyrolyzed, and the heavy metal elements in the oily sludge were solidified. The solidification rate of the heavy metals exceeds 90%, except for vanadium. The reconstituted water-quenched blast furnace slag still has good activity, and it will not affect the further use of the slag after pyrolysis (BFS-P)

Ünlü yönetmen Halit Refiğ'in hayatı kitap oldu:Yorgun Savaşçı filmini İlhan ihbar etti

Taha Toros Arşivi, Dosya No: 119-İlhan SelçukUnutma İstanbul projesi İstanbul Kalkınma Ajansı'nın 2016 yılı "Yenilikçi ve Yaratıcı İstanbul Mali Destek Programı" kapsamında desteklenmiştir. Proje No: TR10/16/YNY/010