Immunohistochemical staining of and Representative fresh-frozen sections of human corneas from donors at various ages (: p16, 18 years; : p21, 33 years; : p27, 54 years; : p53, 18 years; : control, 68 years) are shown

<p><b>Copyright information:</b></p><p>Taken from "Expression of senescence-related genes in human corneal endothelial cells"</p><p></p><p>Molecular Vision 2008;14():161-170.</p><p>Published online 29 Jan 2008</p><p>PMCID:PMC2254959.</p><p></p> Positive staining for all four senescence-related genes are clearly visible in the endothelial nuclei. Magnification; 400×

Quantitative real-time polymerase chain reaction analyses of mRNA expression in human corneal endothelial cells at various ages

<p><b>Copyright information:</b></p><p>Taken from "Expression of senescence-related genes in human corneal endothelial cells"</p><p></p><p>Molecular Vision 2008;14():161-170.</p><p>Published online 29 Jan 2008</p><p>PMCID:PMC2254959.</p><p></p> Total RNA was isolated from HCECs of each group, and the first-strand cDNA were aynthesized from equal amounts of total RNA. Quantitative real-time PCR was performed to analyze mRNA expression. shows the expression of from the eight age groups normalized to mRNA. demonstrates fold change in expression relative to the calibrator of the 20-year-old group. is the comparison of the average level of mRNA expressed in HCECs between the young (≤ 30 years) and old (≥ 50 years). Data are presented as mean±standard deviation, and a