Serial negative response after standard and third (Booster) dose of COVID-19 inactivated vaccine is associated with low vitamin D levels in patients with solid cancers

IntroductionThe response is poorly understood to the third dose in patients with cancer who failed the standard dose of inactivated SARS-CoV-2 vaccines (CoronaVac). We aim to assess the immune response to the third dose and identify whether vitamin D deficiency is associated with serial serologic failure in patients with cancer.MethodsSolid cancer patients (SCP-N) and healthy controls (HCs) who were seronegative after the standard-dose vaccines in our previous study were prospectively recruited, from October 2021 to February 2022, to receive the third dose vaccines and anti-SARS-CoV-2S antibodies were measured. SCP-N who failed the third dose (serial seronegative group, SSG) were matched by propensity scores with the historical standard-dose positive cancer patient group (robust response group, RRG). An exploratory analysis was carried out to validate the role of vitamin D on the serology response.ResultsThe multi-center study recruited 97 SCP-N with 279 positive controls as RRG and 82 negative controls as HC group. The seroconversion rate after third-dose vaccination was higher in SCP-N than in HC (70.6% vs. 29.4%, p < 0.01). The matched comparison showed that patients in SSG had a significantly lower level of vitamin D and consumption rate than RRG or RRG-B (RRG with third-dose positive) (all p < 0.01). None had serious (over grade II) adverse events after the third dose.ConclusionSolid cancer patients with second-dose vaccine failure may have a relatively poor humoral response to the third dose of COVID-19 vaccines as compared with the seronegative HC group. The consecutively poor humoral response could be associated with poor vitamin D levels and intake. Vitamin D status and cancer-related immune compromise may jointly affect the humoral response following booster vaccination

Penaeid shrimp genome provides insights into benthic adaptation and frequent molting

Crustacea, the subphylum of Arthropoda which dominates the aquatic environment, is of major importance in ecology and fisheries. Here we report the genome sequence of the Pacific white shrimp Litopenaeus vannamei, covering similar to 1.66 Gb (scaffold N50 605.56 Kb) with 25,596 protein-coding genes and a high proportion of simple sequence repeats (>23.93%). The expansion of genes related to vision and locomotion is probably central to its benthic adaptation. Frequent molting of the shrimp may be explained by an intensified ecdysone signal pathway through gene expansion and positive selection. As an important aquaculture organism, L. vannamei has been subjected to high selection pressure during the past 30 years of breeding, and this has had a considerable impact on its genome. Decoding the L. vannamei genome not only provides an insight into the genetic underpinnings of specific biological processes, but also provides valuable information for enhancing crustacean aquaculture

Neuroprotective Mechanisms of Lycium barbarum Polysaccharides Against Ischemic Insults by Regulating NR2B and NR2A Containing NMDA Receptor Signaling Pathways

Glutamate excitotoxicity plays an important role in neuronal death after ischemia. However, all clinical trials using glutamate receptor inhibitors have failed. This may be related to the evidence that activation of different subunit of NMDA receptor will induce different effects. Many studies have shown that activation of the intrasynaptic NR2A subunit will stimulate survival signaling pathways, whereas upregulation of extrasynaptic NR2B will trigger apoptotic pathways. A Lycium barbarum polysaccharide (LBP) is a mixed compound extracted from Lycium barbarum fruit. Recent studies have shown that LBP protects neurons against ischemic injury by anti-oxidative effects. Here we first reported that the effect of LBP against ischemic injury can be achieved by regulating NR2B and NR2A signaling pathways. By in vivo study, we found LBP substantially reduced CA1 neurons from death after transient global ischemia and ameliorated memory deficit in ischemic rats. By in vitro study, we further confirmed that LBP increased the viability of primary cultured cortical neurons when exposed to oxygen-glucose deprivation (OGD) for 4 h. Importantly, we found that LBP antagonized increase in expression of major proteins in the NR2B signal pathway including NR2B, nNOS, Bcl-2-associated death promoter (BAD), cytochrome C (cytC) and cleaved caspase-3, and also reduced ROS level, calcium influx and mitochondrial permeability after 4 h OGD. In addition, LBP prevented the downregulation in the expression of NR2A, pAkt and pCREB, which are important cell survival pathway components. Furthermore, LBP attenuated the effects of a NR2B co-agonist and NR2A inhibitor on cell mortality under OGD conditions. Taken together, our results demonstrated that LBP is neuroprotective against ischemic injury by its dual roles in activation of NR2A and inhibition of NR2B signaling pathways, which suggests that LBP may be a superior therapeutic candidate for targeting glutamate excitotoxicity for the treatment of ischemic stroke

Charakterisierung und Regulation des stress associated proteins (SAP) 12 unter abiotischem Stress in Arabidopsis thaliana

Wang X. Charakterisierung und Regulation des stress associated proteins (SAP) 12 unter abiotischem Stress in Arabidopsis thaliana. Bielefeld; 2017

Research on Sustainable Closed-Loop Supply Chain Synergy in Forest Industry Based on High-Quality Development: A Case Study in Northeast China

Forestry construction is a fundamental issue related to sustainable economic and social development. However, the traditional development of forestry is currently facing the dilemma of insufficient resource supply, rising labor cost, and a low return on forestry investment, which needs to be transformed into high-quality forestry development. The general view is that synergistic development is an important way to achieve high-quality development. Based on this, the strategic planning and behavioral choices of forestry enterprises under synergistic development are explored. With the objectives of minimizing cost and environmental impacts, and maximizing social benefits, a multi-objective sustainable closed-loop supply chain network planning model is developed to study a real case of a forestry supply chain in northeastern China. A robust optimization approach is used to deal with the relevant uncertain parameters, and a weighted generalized epsilon-constraint approach is applied to solve the multi-objective problem, based on which a GA genetic algorithm is used to solve it. Based on the synergistic development perspective, four different scenario assumptions and sensitivity analyses are made to examine the multi-objective calculation results in this closed-loop supply chain network, and then to analyze the strategic decisions and specific measures for forest industry enterprises to achieve high-quality development. The model results show that realizing supply chain synergy is an effective way to achieve efficient business development in the future. Managers should also establish an acceptable balance between sustainability dimensions while focusing on building a collaborative supply chain development model, where small economic benefits can be appropriately ignored to reduce the harmful effects of the production process on the environment

Effects of B-site Co2O3 doping on microstructure and electrical properties of Na0.25K0.25Bi2.5Nb2O9 ceramics

The effects of cobalt addition on the properties of Na0.25K0.25Bi2.5Nb2O9 (NKBN) ebased ceramics have been investigated in details. It was found that the ceramics possess a pure phase of bismuth oxide layer etype structure. The Curie temperature T-c gradually increases from 653 degrees C to 662 degrees C with increasing the Co modification. The electrical properties of NKBNebased ceramics are improved significantly by the addition of Co. The piezoelectric constant d33, dielectric loss tan d, mechanical quality factor Q(m) and remanent polarization Pr for the NKBN ceramics with 0.20 wt% Co2O3 modification were found to be 23 pC/N, 0.35%, 3028, 12.03 mC/cm(2), respectively. Thermal annealing studies indicated that the cobalt emodified NKBN ceramics system possesses stable piezoelectric properties, demonstrating that the cobaltemodified NKBNebased ceramics are the promising candidates for highetemperature applications. (C) 2015 Elsevier B.V. All rights reserved

Forecasting and Coupled Coordination Analysis of Supply and Demand for Sustainable Talent in Chinese Agriculture

In recent years, China has achieved notable results with its poverty alleviation program, the focus of which is shifting toward the comprehensive promotion of rural revitalization. The role played by sustainable human resources in agriculture is becoming increasingly prominent. In this context, China’s sustainable talent in agriculture is used as the research object, and a neural network analysis method is applied to construct a prediction model of sustainable agricultural talent to forecast its supply and demand. The prediction aims to provide a scientific basis for the strategic planning of talent development for rural revitalization. Based on the forecast results by region and province, we analyzed the level of coordinated development of talent supply and demand to provide a reference for the coordinated development of supply and demand of sustainable talent in agriculture in China. The results showed that a large sustainable agricultural talent demand gap exists in China. The overall talent supply and demand coupling coordination level is low; we found significant differences among different regions and provinces, characterized by decreasing order of the northeast, central, west, and east. According to the socio-economic development level, agricultural economic foundation, and other factors, we divided the provinces into six types for analysis. To promote the coordinated development of sustainable human agricultural resources, talent policy support at the national level is required to reduce the loss of human resources to other countries; at the regional level, the talent environment for rural revitalization should be optimized to increase the attraction of talent. At the provincial level, agricultural and forestry education resources should be created to increase the supply of sustainable agricultural talent