Role of (p)ppGpp in Viability and Biofilm Formation of Actinobacillus pleuropneumoniae S8.

Actinobacillus pleuropneumoniae is a Gram-negative bacterium and the cause of porcine pleuropneumonia. When the bacterium encounters nutritional starvation, the relA-dependent (p)ppGpp-mediated stringent response is activated. The modified nucleotides guanosine 5'-diphosphate 3'-diphosphate (ppGpp) and guanosine 5'-triphosphate 3'-diphosphate (pppGpp) are known to be signaling molecules in other prokaryotes. Here, to investigate the role of (p)ppGpp in A. pleuropneumoniae, we created a mutant A. pleuropneumoniae strain, S8ΔrelA, which lacks the (p)ppGpp-synthesizing enzyme RelA, and investigated its phenotype in vitro. S8ΔrelA did not survive after stationary phase (starvation condition) and grew exclusively as non-extended cells. Compared to the wild-type (WT) strain, the S8ΔrelA mutant had an increased ability to form a biofilm. Transcriptional profiles of early stationary phase cultures revealed that a total of 405 bacterial genes were differentially expressed (including 380 up-regulated and 25 down-regulated genes) in S8ΔrelA as compared with the WT strain. Most of the up-regulated genes are involved in ribosomal structure and biogenesis, amino acid transport and metabolism, translation cell wall/membrane/envelope biogenesis. The data indicate that (p)ppGpp coordinates the growth, viability, morphology, biofilm formation and metabolic ability of A. pleuropneumoniae in starvation conditions. Furthermore, S8ΔrelA could not use certain sugars nor produce urease which has been associated with the virulence of A. pleuropneumoniae, suggesting that (p)ppGpp may directly or indirectly affect the pathogenesis of A. pleuropneumoniae during the infection process. In summary, (p)ppGpp signaling represents an essential component of the regulatory network governing stress adaptation and virulence in A. pleuropneumoniae

The Technical and Economical Analysis of a Centralized Air-Conditioning System with Cold Storage Refrigeration in High-Rise Residential Buildings

In recent years, the application of a centralized air-conditioning system (CACS) with cold storage refrigeration in high-rise residential buildings has gradually increased. Due to the large difference between civil residential buildings and commercial buildings, characteristics such as the cooling load in summer and the storey height must be considered in the design of the air-conditioning system, and the cold storage equipment and the cold supplying means must be properly selected. The option of establishing centralized air-conditioning equipment with cold storage and supplying unified cold in high-rise residential buildings is analyzed objectively with technical and economical methods in this paper. It is not true that the option of supplying unified cold can save energy all the time. CACS with cold storage will not always be economical. Based on a 27-floor building, the running costs in summer and the first costs are both compared between CACS with and without cold storage refrigeration. The cold storage method selected will significantly impact the residents

Mechanically-Induced Transport Switching Effect in Graphene-based Nanojunctions

We report a theoretical study suggesting a novel type of electronic switching effect, driven by the geometrical reconstruction of nanoscale graphene-based junctions. We considered junction struc- tures which have alternative metastable configurations transformed by rotations of local carbon dimers. The use of external mechanical strain allows a control of the energy barrier heights of the potential profiles and also changes the reaction character from endothermic to exothermic or vice-versa. The reshaping of the atomic details of the junction encode binary electronic ON or OFF states, with ON/OFF transmission ratio that can reach up to 10^4-10^5. Our results suggest the possibility to design modern logical switching devices or mechanophore sensors, monitored by mechanical strain and structural rearrangements.Comment: 10 pages, 4 figure

Dense-coding quantum key distribution based on continuous-variable entanglement

We proposed a scheme of continuous-variable quantum key distribution, in which the bright Einstein-Podolsky-Rosen entangled optical beams are utilized. The source of the entangled beams is placed inside the receiving station, where half of the entangled beams are transmitted with round trip and the other half are retained by the receiver. The amplitude and phase signals modulated on the signal beam by the sender are simultaneously extracted by the authorized receiver with the scheme of the dense-coding correlation measurement for continuous quantum variables, thus the channel capacity is significantly improved. Two kinds of possible eavesdropping are discussed. The mutual information and the secret key rates are calculated and compared with those of unidirectional transmission schemes

Integration of crosswind forces into train dynamic modelling

In this paper a new method is used to calculate unsteady wind loadings acting on a railway vehicle. The method takes input data from wind tunnel testing or from computational fluid dynamics simulations (one example of each is presented in this article), for aerodynamic force and moment coefficients and combines these with fluctuating wind velocity time histories and train speed to produce wind force time histories on the train. This method is fast and efficient and this has allowed the wind forces to be applied to a vehicle dynamics simulation for a long length of track. Two typical vehicles (one passenger, one freight) have been modelled using the vehicle dynamics simulation package ‘VAMPIRE®’, which allows detailed modelling of the vehicle suspension and wheel—rail contact. The aerodynamic coefficients of the passenger train have been obtained from wind tunnel tests while those of the freight train have been obtained through fluid dynamic computations using large-eddy simulation. Wind loadings were calculated for the same vehicles for a range of average wind speeds and applied to the vehicle models using a user routine within the VAMPIRE package. Track irregularities measured by a track recording coach for a 40 km section of the main line route from London to King's Lynn were used as input to the vehicle simulations. The simulated vehicle behaviour was assessed against two key indicators for derailment; the Y/Q ratio, which is an indicator of wheel climb derailment, and the Δ Q/Q value, which indicates wheel unloading and therefore potential roll over. The results show that vehicle derailment by either indicator is not predicted for either vehicle for any mean wind speed up to 20 m/s (with consequent gusts up to around 30 m/s). At a higher mean wind speed of 25 m/s derailment is predicted for the passenger vehicle and the unladen freight vehicle (but not for the laden freight vehicle)

Radiation-Induced "Zero-Resistance State" and the Photon Assisted Transport

We demonstrate that the radiation induced "zero-resistance state" observed in a two-dimensional electron gas is a result of the non-trivial structure of the density of states of the systems and the photon assisted transport. A toy model of a structureless quantum tunneling junction where the system has oscillatory density of states catches most of the important features of the experiments. We present a generalized Kubo-Greenwood conductivity formula for the photon assisted transport in a general system, and show essentially the same nature of the transport anomaly in a uniform system.Comment: 4 pages, 3 figures. Please send comment to [email protected]. This version added a paragraph to discuss the implication of negative conductanc

Historic Light Curve and Long-term Optical Variation of BL Lacertae 2200+420

In this paper, historical optical(UBVRI) data and newly observed data from the Yunnan Observatory of China(about100 years) are presented for BL Lacertae. Maximum variations in UBVRI: 5.12, 5.31, 4.73, 2.59, and 2.54 and color indices of U-B = -0.11 +/- 0.20, B-V= 1.0 +/- 0.11, V-R= 0.73 +/- 0.19, V-I= 1.42 +/- 0.25, R-I= 0.82 +/- 0.11, and B-I= 2.44 +/- 0.29 have been obtained from the literature; The Jurkevich method is used to investigate the existence of periods in the B band light curve, and a long-term period of 14 years is found. The 0.6 and 0.88 year periods reported by Webb et al.(1988) are confirmed. In addition, a close relation between B-I and B is found, suggesting that the spectra flattens when the source brightens.Comment: 21 pages, 6 figures, 2 table, aasms4.sty, to be published in ApJ, Vol. 507, 199