Accumulation Pattern of Flavonoids in Cabernet Sauvignon Grapes Grown in a Low-Latitude and High-Altitude Region

Particular climate conditions in a low-latitude and high-altitude region endow grape berries with distinctivequality characteristics. So far, few reports have been concerned with the formation of berry flavour in such aregion. This study aimed to investigate the accumulation pattern of flavonoids in Vitis vinifera L. cv. CabernetSauvignon grape berries growing at different altitudes of the highland in southwest China in two consecutivevintages. In addition to the 3-O-monoglucosides and 3-O-acyl monoglucosides of the five main anthocyanidins(delphinidin, cyanidin, peonidin, petunidin and malvidin), some uncommon anthocyanins, such as threediglucosides of anthocyanidins and pelargonidin-3-O-glucoside, were detected in the grape berries. Higheraltitude cultivation greatly promoted the production of anthocyanins and flavonols, particularly cyanidintypeanthocyanins and quercetin-type flavonols from the F3’H branch of the flavonoid biosynthetic pathway.Flavan-3-ols from both branches were comparatively less influenced by vineyard altitude. Vintage in thishigh-altitude region also had a dramatic influence on the accumulation of flavonoids. Most of the anthocyaninand flavonol components were affected more by vineyard altitude than by vintage, whereas the accumulationof flavan-3-ols differed mainly between vintages. The present data will not only improve the understandingof flavonoid accumulation in grapes from a high-altitude region with different climates, but also providepractical guidance for the production of high-quality grapes and wine

Combination of capecitabine and ludartin inhibits colon cancer growth in mice

Purpose: To investigate the efficacy of capecitabine and ludartin in the treatment of colon cancer in mice.Methods: Mice model of colon cancer was used in this study. Quantitative real-time polymerase chain reaction (Qrt-PCR) was used to quantify the expression of vascular endothelial growth factor (VEGF) mRNA. Micro-vessel density was assessed using immunohistochemical analysis.Results: When administered separately, capecitabine and ludartin treatments significantly suppressed tumor growth in the mice model of colon cancer for 4 weeks, compared to control group. Coadministration of capecitabine and ludartin significantly inhibited tumor growth for 6 weeks (p < 0.05). Symptoms of colon cancer such as weight loss, skin discoloration and leukopenia were observed in untreated control group. However, these symptoms were completely absent in the group treated with combination of capecitabine and ludartin. The combined treatment also prevented colon cancer-induced increase in white blood cell (WBC) count, and increased median survival time of colon cancer mice from 38 to 55 days. Expression of VEGF in combination (capecitabine + ludartin) treatment group was significantly lower than in the control, i.e., untreated group (p ˂ 0.05). The combination treatment group also had significantly lower micro-vessel density in the tumor tissues, compared to the  ntreated control mice (p < 0.05).Conclusion: These results show that a combination treatment of capecitabine and ludartin effectively inhibits colon tumor growth and angiogenesis in mice via a mechanism involving suppression of VEGF expression. Thus, capecitabine and ludartin combination is a potentially  uitable treatment for colon cancer.Keywords: Colon cancer, Mice, Ludartin, Leukopenia, VEGF expression, Angiogenesi

Age-Related Differences in Accelerometer-Assessed Physical Activity and Sleep Parameters Among Children and Adolescents With and Without Autism Spectrum Disorder: A Meta-Analysis

Importance: Physical inactivity and sleep disorders are health-related concerns of youth with autism spectrum disorder (ASD) that can persist from childhood and exacerbate core symptoms. However, evidence on group differences in accelerometer-assessed physical activity and sleep parameters among youth with and without ASD is inconclusive and age-specific effects remain unclear. Objective: To synthesize evidence on group differences in accelerometer-assessed physical activity and sleep parameters and examine the moderating effects of age between children and adolescents with and without ASD. Data Sources: American Psychological Association PsychInfo, CINAHL Ultimate, ERIC, MEDLINE, SPORTDiscus with Full Text, and Web of Science from inception to February 2023. Study Selection: Two independent reviewers screened articles for observational research comparing accelerometer-assessed physical activity levels and sleep parameters in children and adolescents with and without ASD. Data Extraction and Synthesis: After developing a standardized form, relevant data were extracted. Quality was assessed using the McMaster Critical Review Form and rated based on sample, measurement, and analyses. The Preferring Reporting Items for Systematic Reviews and Meta-Analyses guideline was followed. Main Outcomes and Measures: The primary outcomes were actigraphy-measured MVPA, sleep latency, sleep efficiency, total sleep time, and wake after sleep onset. Data were pooled using a random-effects model. Hedges g was used to express the effect size index. Meta-regression on age was also performed to investigate the potential moderating effects. Results: Collectively, 1757 studies were initially identified. Among 104 articles that were assessed, 28 were included, comprising moderate-to-vigorous physical activity (MVPA), 4 sleep parameters, and 73 independent effect sizes. A total of 28 studies were included in analysis, comprising 805 children and adolescents with ASD and 1573 without ASD (age range, 5.1-16.9 years). Compared with peers without ASD, children and adolescents with ASD had a small-to-moderate difference in MVPA (g = −0.450; 95% CI, −0.622 to −0.277), total sleep time (g = −0.332; 95% CI, −0.574 to −0.090), sleep efficiency (g = −0.424; 95% CI, −0.645 to −0.203), and a moderate difference in sleep latency (g = 0.514; 95% CI, 0.351 to 0.677) measured by actigraphy. Children and adolescents with ASD experienced an age-related decline in moderate-to-vigorous physical activity (β = −0.049 [95% CI, −0.097 to −0.001]; P = .045), indicating that younger children with ASD showed a smaller difference in MVPA compared with their peers without ASD. Moderating effects of age on sleep parameters were not significant. Conclusions and Relevance: The findings of this meta-analysis suggested that children and adolescents with ASD had lower MVPA and worse sleep than peers without ASD, and the difference in MVPA varied with age. These findings reinforce the need for public health initiatives aimed at reducing these group disparities

Galactic Disk Bulk Motions as Revealed by the LSS-GAC DR2

We report a detailed investigation of the bulk motions of the nearby Galactic stellar disk, based on three samples selected from the LSS-GAC DR2: a global sample containing 0.57 million FGK dwarfs out to $\sim$ 2 kpc, a local subset of the global sample consisting $\sim$ 5,400 stars within 150 pc, and an anti-center sample containing $\sim$ 4,400 AFGK dwarfs and red clump stars within windows of a few degree wide centered on the Galactic anti-center. The global sample is used to construct a three-dimensional map of bulk motions of the Galactic disk from the solar vicinity out to $\sim$ 2 kpc with a spatial resolution of $\sim$ 250 pc. Typical values of the radial and vertical components of bulk motion range from $-$15 km s$^{-1}$ to 15 km s$^{-1}$, while the lag behind the circular speed dominates the azimuthal component by up to $\sim$ 15 km s$^{-1}$. The map reveals spatially coherent, kpc-scale stellar flows in the disk, with typical velocities of a few tens km s$^{-1}$. Bending- and breathing-mode perturbations are clearly visible, and vary smoothly across the disk plane. Our data also reveal higher-order perturbations, such as breaks and ripples, in the profiles of vertical motion versus height. From the local sample, we find that stars of different populations exhibit very different patterns of bulk motion. Finally, the anti-center sample reveals a number of peaks in stellar number density in the line-of-sight velocity versus distance distribution, with the nearer ones apparently related to the known moving groups. The "velocity bifurcation" reported by Liu et al. (2012) at Galactocentric radii 10--11 kpc is confirmed. However, just beyond this distance, our data also reveal a new triple-peaked structure.Comment: 27 pages, 17 figures, Accepted for publication in a special issue of Research in Astronomy and Astrophysics on LAMOST science

GSK3 Inhibitor-BIO Regulates Proliferation of Immortalized Pancreatic Mesenchymal Stem Cells (iPMSCs)

<div><h3>Background</h3><p>The small molecule 6-bromoindirubin-30-oxime (BIO), a glycogen synthase kinase 3 (GSK3) inhibitor, is a pharmacological agent known to maintain self-renewal in human and mouse embryonic stem cells (ESCs). However, the precise role of GSK3 in immortalized pancreatic mesenchymal stem cells (iPMSCs) growth and survival is not completely understood at present.</p> <h3>Results</h3><p>To determine whether this molecule is involved in controlling the proliferation of iPMSCs, we examined the effect of BIO on iPMSCs. We found that the inactivation of GSK3 by BIO can robustly stimulate iPMSCs proliferation and mass formation as shown by QRT-PCR, western blotting, 5-Bromo-2-deoxyuridine (BrdU) immunostaining assay and tunel assay. However, we did not find the related roles of BIO on β cell differentiation by immunostaining, QRT-PCR assay, glucose-stimulated insulin release and C-peptide content analysis.</p> <h3>Conclusions</h3><p>These results suggest that BIO plays a key role in the regulation of cell mass proliferation and maintenance of the undifferentiated state of iPMSCs.</p> </div

NASA-Approved Rotary Bioreactor Enhances Proliferation of Human Epidermal Stem Cells and Supports Formation of 3D Epidermis-Like Structure

The skin is susceptible to different injuries and diseases. One major obstacle in skin tissue engineering is how to develop functional three-dimensional (3D) substitute for damaged skin. Previous studies have proved a 3D dynamic simulated microgravity (SMG) culture system as a “stimulatory” environment for the proliferation and differentiation of stem cells. Here, we employed the NASA-approved rotary bioreactor to investigate the proliferation and differentiation of human epidermal stem cells (hEpSCs). hEpSCs were isolated from children foreskins and enriched by collecting epidermal stem cell colonies. Cytodex-3 micro-carriers and hEpSCs were co-cultured in the rotary bioreactor and 6-well dish for 15 days. The result showed that hEpSCs cultured in rotary bioreactor exhibited enhanced proliferation and viability surpassing those cultured in static conditions. Additionally, immunostaining analysis confirmed higher percentage of ki67 positive cells in rotary bioreactor compared with the static culture. In contrast, comparing with static culture, cells in the rotary bioreactor displayed a low expression of involucrin at day 10. Histological analysis revealed that cells cultured in rotary bioreactor aggregated on the micro-carriers and formed multilayer 3D epidermis structures. In conclusion, our research suggests that NASA-approved rotary bioreactor can support the proliferation of hEpSCs and provide a strategy to form multilayer epidermis structure