The mechanism of high contents of oil and oleic acid revealed by transcriptomic and lipidomic analysis during embryogenesis in Carya cathayensis Sarg.

Gene transcription in lipid synthesis. (XLSX 55 kb

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

Emerging single‐atom catalysts in electrochemical biosensing

Abstract Single‐atom catalysts (SACs) have attracted extensive interest owing to their maximized atomic utilization, low cost as well as outstanding catalytic activity, selectivity, and stability for diverse applications. Due to their excellent performance in electrocatalysis, SACs can be applied to electrochemical sensors, which have been a predominant tool employed in biosensing. In very recent studies, SAC‐based electrochemical biosensors have demonstrated enhanced sensing performances in biomarker detection and in vivo analysis. However, a comprehensive review of SAC‐based electrochemical biosensors has not been reported yet. Herein, we present a summary of the synthesis methods of SACs with their application in electrochemical sensor establishment and electrochemical characterization methods in electrochemical sensing. Biomedical applications utilizing SAC‐based electrochemical biosensors are introduced. Finally, the existing challenges and future prospects of SACs in the field of electrochemical biosensing are discussed

Relationships between Combustion Behavior in Air and the Chemical Structure of Bituminous Coal during Combustion Processes

The structural parameters of five bituminous coals were analyzed by using X-ray diffraction and attenuated total reflection–Fourier transform infrared spectroscopy. The combustion behavior of coal was investigated by using a thermogravimetric analyzer under air conditions. Furthermore, the relationships between combustion parameters and the coal structure were established. The results show that bituminous coals contain crystalline and amorphous carbon. The aromaticity, interlayer spacing, average stacking height, aliphatic chain length, and the hydrocarbon-generating potential varied with the different bituminous coals. The coal samples exhibited similar weight changes during the combustion process, and the combustion parameters increased with increments in heating rate. The maximum combustion rate and activation energy increased with declining interlayer spacing and hydrocarbon-generating potential and increasing aromaticity, average stacking height, and aliphatic chain length. The bituminous coal for the utilization of combustion should have high aromaticity, a degree of graphitization, crystalline, long aliphatic chain length, and weak hydrocarbon-generating potential

Estimation of Forest Aboveground Biomass in Changbai Mountain Region Using ICESat/GLAS and Landsat/TM Data

Mapping the magnitude and spatial distribution of forest aboveground biomass (AGB, in Mg·ha−1) is crucial to improve our understanding of the terrestrial carbon cycle. Landsat/TM (Thematic Mapper) and ICESat/GLAS (Ice, Cloud, and land Elevation Satellite, Geoscience Laser Altimeter System) data were integrated to estimate the AGB in the Changbai Mountain area. Firstly, four forest types were delineated according to TM data classification. Secondly, different models for prediction of the AGB at the GLAS footprint level were developed from GLAS waveform metrics and the AGB was derived from field observations using multiple stepwise regression. Lastly, GLAS-derived AGB, in combination with vegetation indices, leaf area index (LAI), canopy closure, and digital elevation model (DEM), were used to drive a data fusion model based on the random forest approach for extrapolating the GLAS footprint AGB to a continuous AGB map. The classification result showed that the Changbai Mountain region was characterized as forest-rich in altitudinal vegetation zones. The contribution of remote sensing variables in modeling the AGB was evaluated. Vegetation index metrics account for large amount of contribution in AGB ranges <150 Mg·ha−1, while canopy closure has the largest contribution in AGB ranges ≥150 Mg·ha−1. Our study revealed that spatial information from two sensors and DEM could be combined to estimate the AGB with an R2 of 0.72 and an RMSE of 25.24 Mg·ha−1 in validation at stand level (size varied from ~0.3 ha to ~3 ha)

Human spatial navigation: Neural representations of spatial scales and reference frames obtained from an ALE meta-analysis

Humans use different spatial reference frames (allocentric or egocentric) to navigate successfully toward their destination in different spatial scale spaces (environmental or vista). However, it remains unclear how the brain represents different spatial scales and different spatial reference frames. Thus, we conducted an activation likelihood estimation (ALE) meta-analysis of 47 fMRI articles involving human spatial navigation. We found that both the environmental and vista spaces activated the parahippocampal place area (PPA), retrosplenial complex (RSC), and occipital place area in the right hemisphere. The environmental space showed stronger activation than the vista space in the occipital and frontal regions. No brain region exhibited stronger activation for the vista than the environmental space. The allocentric and egocentric reference frames activated the bilateral PPA and right RSC. The allocentric frame showed more stronger activations than the egocentric frame in the right culmen, left middle frontal gyrus, and precuneus. No brain region displayed stronger activation for the egocentric than the allocentric navigation. Our findings suggest that navigation in different spatial scale spaces can evoke specific and common brain regions, and that the brain regions representing spatial reference frames are not absolutely separated

Solid or Liquid Food—The Intention to Eat Different Foods under Negative Emotions

Food can relieve an individual’s emotions, especially for emotional eaters. For instance, chewing alleviates negative emotions. Solid and liquid foods comprise a huge part of our daily lives, and the chewiness of solid foods is always high. Here, we explored whether people, especially emotional eaters, have higher eating intentions to eat highly chewy foods while experiencing negative emotions by comparing their eating intentions toward solid and liquid foods. To this end, we conducted a survey of 147 participants using a questionnaire (Experiment 1) to understand their eating intention toward five types of food (purple potato, maize, black soya bean, mango, and soybean; each food group contained a solid food and a liquid food) while experiencing negative emotions. The results showed that individuals exhibited higher eating intention toward solid food compared with liquid food while experiencing negative emotions. In Experiment 2, we selected 85 and 65 high-emotional and low-emotional eaters, respectively, and further explored their preference for solid foods. The results showed that individuals with high levels of emotional eating exhibited higher intentions toward solid food while experiencing negative emotions compared with those with low levels of emotional eating. In conclusion, this study proved that individuals’ higher eating intentions toward highly chewable food were pronounced among individuals with high levels of emotional eating under negative emotion conditions

A new insight into male fertility preservation for patients with completely immotile spermatozoa

Abstract Background Sperm cryopreservation is the most effective method to preserve male fertility but this is normally used for motile spermatozoa. Thus, only motile spermatozoa are used for cryopreservation in most reproductive medicine centers worldwide. The immotile spermatozoa from some problematic patients are usually discarded, resulting in a missed opportunity of sterility cryopreservation for future assisted reproductive treatments. Many studies have shown that successful fertilization can be obtained after selection of viable sperm from the completely immotile spermatozoa before ICSI. Whether the completely immotile spermatozoa are worth of freezing has not been realized The aim of this study is to explore the clinical value of cryopreservation of immotile spermatozoa. Methods Completely immotile spermatozoa were collected and frozen, and subsequently viable but immotile frozen-thawed spermatozoa were selected by laser plus for ICSI. Main outcomes included spermatozoa survival index, fertilization rate and good quality embryo rate. Results After identification by laser, the fresh samples of spermatozoa presented with a mean survival rate of 54.86% and 26.05%, and this was reduced to 44.13% and 18.13% in frozen-thawed spermatozoa samples, which showed a frozen-thawed spermatozoa survival index of 0.80 and 0.70 in the testicular and ejaculate sperm, respectively. There were no statistically differences in fertilization rate (80% vs80.51%, 75.00% vs 81.48%), cleavage rate (95.45% vs 98.95%, 100.00% vs 95.45%) and good quality embryo rate (40.48% vs 52.13%, 33.33%vs38.10%) between the frozen-thawed immotile spermatozoa group and the routine fresh immotile spermatozoa ICSI group in both testicular and ejaculate sperm, respectively. Conclusions The results of the study show that completely immotile spermatozoa can be frozen in order to preserve male fertility as long as viable spermatozoa are present. This procedure provides a further possibility for fertility preservation for patients with completely immotile spermatozoa