23 research outputs found

    The Effects of Hydrogen Peroxide on the Circadian Rhythms of Microcystis aeruginosa

    Get PDF
    Background: The cyanobacterium Microcystis aeruginosa is one of the principal bloom-forming cyanobacteria present in a wide range of freshwater ecosystems. M. aeruginosa produces cyanotoxins, which can harm human and animal health. Many metabolic pathways in M. aeruginosa, including photosynthesis and microcystin synthesis, are controlled by its circadian rhythms. However, whether xenobiotics affect the cyanobacterial circadian system and change its growth, physiology and biochemistry is unknown. We used real-time PCR to study the effect of hydrogen peroxide (H2O2) on the expression of clock genes and some circadian genes in M. aeruginosa during the light/dark (LD) cycle. Results: The results revealed that H 2O 2 changes the expression patterns of clock genes (kaiA, kaiB, kaiC and sasA) and significantly decreases the transcript levels of kaiB, kaiC and sasA. H2O2 treatment also decreased the transcription of circadian genes, such as photosynthesis-related genes (psaB, psbD1 and rbcL) and microcystin-related genes (mcyA, mcyD and mcyH), and changed their circadian expression patterns. Moreover, the physiological functions of M. aeruginosa, including its growth and microcystin synthesis, were greatly influenced by H 2O 2 treatment during LD. These results indicate that changes in the cyanobacterial circadian system can affect its physiological and metabolic pathways. Conclusion: Our findings show that a xenobiotic can change the circadian expression patterns of its clock genes t

    Scheduling multi–mode resource–constrained tasks of automated guided vehicles with an improved particle swarm optimization algorithm

    No full text
    Abstract A modified particle swarm optimization (PSO) approach is presented for the multi‐mode resource‐constrained scheduling problem of automated guided vehicle (AGV) tasks. Various constraints in the scheduling process of the AGV system are analysed, and the types and quantities of AGVs as allocable resources are considered. The multiple‐AGV combined distribution mode and its impact on distribution tasks is also considered. Finally, a multi‐mode resource‐constrained task scheduling model is established for which the object is to minimise material delivery time. Based on the above model, the discrete particle swarm optimization algorithm that improved the basic PSO was proposed. The simulation results with the test set in PSPLIB standard library showed the effectiveness of the improved PSO algorithm

    Insights into the transcriptional responses of a microbial community to silver nanoparticles in a freshwater microcosm.

    Get PDF
    Silver nanoparticles (AgNPs) are widely used because of their excellent antibacterial properties. They are, however, easily discharged into the water environment, causing potential adverse environmental effects. Meta-transcriptomic analyses are helpful to study the transcriptional response of prokaryotic and eukaryotic aquatic microorganisms to AgNPs. In the present study, microcosms were used to investigate the toxicity of AgNPs to a natural aquatic microbial community. It was found that a 7-day exposure to 10 μg L-1 silver nanoparticles (AgNPs) dramatically affected the structure of the microbial community. Aquatic micro eukaryota (including eukaryotic algae, fungi, and zooplankton) and bacteria (i.e., heterotrophic bacteria and cyanobacteria) responded differently to the AgNPs stress. Meta-transcriptomic analyses demonstrated that eukaryota could use multiple cellular strategies to cope with AgNPs stress, such as enhancing nitrogen and sulfur metabolism, over-expressing genes related to translation, amino acids biosynthesis, and promoting bacterial-eukaryotic algae interactions. By contrast, bacteria were negatively affected by AgNPs with less signs of detoxification than in case of eukaryota; various pathways related to energy metabolism, DNA replication and genetic repair were seriously inhibited by AgNPs. As a result, eukaryotic algae (mainly Chlorophyta) dominated over cyanobacteria in the AgNPs treated microcosms over the 7-d exposure. The present study helps to understand the effects of AgNPs on aquatic microorganisms and provides insights into the contrasting AgNPs toxicity in eukaryota and bacteria

    Photosynthesis-related gene transcripts in <i>M. aeruginosa</i> under light and dark conditions after H<sub>2</sub>O<sub>2</sub> treatment.

    No full text
    <p>The shaded areas correspond to the dark period. Symbols represent mean ± SEM of triplicate cultures. The mRNA amount of all examined genes is normalized to 16S rDNA. The open circle corresponds to the control treatment; the filled circle corresponds to the L0 treatment; the filled square corresponds to the D0 treatment. A: The effect of H<sub>2</sub>O<sub>2</sub> on <i>psaB</i> circadian transcript; B: the effect of H<sub>2</sub>O<sub>2</sub> on the <i>psbD1</i> circadian transcript; C: the effect of H<sub>2</sub>O<sub>2</sub> on the <i>rbcL</i> circadian transcript.</p

    Growth of <i>M. aeruginosa</i> that was cultured with H<sub>2</sub>O<sub>2</sub> in the first 24 h.

    No full text
    <p>The shaded areas correspond to the dark period. Symbols represent mean ± SEM of triplicate cultures. The open circle corresponds to the control treatment; the filled circle corresponds to the L0 treatment; the filled square corresponds to the D0 treatment.</p

    The clock gene transcripts in <i>M. aeruginosa</i> under light and dark conditions after H<sub>2</sub>O<sub>2</sub> treatment.

    No full text
    <p>The shaded areas correspond to the dark period. Symbols represent mean ± SEM of triplicate cultures. The mRNA amount of all examined genes is normalized to 16S rDNA. The open circle corresponds to the control treatment; the filled circle corresponds to the L0 treatment; the filled square corresponds to the D0 treatment. A, The effect of H<sub>2</sub>O<sub>2</sub> on the <i>kaiA</i> circadian transcript; B, the effect of H<sub>2</sub>O<sub>2</sub> on the <i>kaiB</i> circadian transcript; C: the effect of H<sub>2</sub>O<sub>2</sub> on the <i>kaiC</i> circadian transcript; D: the effect of H<sub>2</sub>O<sub>2</sub> on the <i>sasA</i> circadian transcript.</p

    The intracellular microcystin content of <i>M. aeruginosa</i> under light and dark conditions after H<sub>2</sub>O<sub>2</sub> treatment.

    No full text
    <p>The shaded areas correspond to the dark period. The symbols represent the mean ± SEM of triplicate cultures. The filled circle corresponds to the L0 treatment; the open circle corresponds to the control treatment; the filled square corresponds to the D0 treatment.</p

    The change of microcystin content in water after oxidation by H<sub>2</sub>O<sub>2</sub> for various time periods.

    No full text
    <p>The change of microcystin content in water after oxidation by H<sub>2</sub>O<sub>2</sub> for various time periods.</p
    corecore