Binder materials for the cathodes applied to self-stratifying membraneless microbial fuel cell

© 2018 The Authors The recently developed self-stratifying membraneless microbial fuel cell (SSM-MFC) has been shown as a promising concept for urine treatment. The first prototypes employed cathodes made of activated carbon (AC) and polytetrafluoroethylene (PTFE) mixture. Here, we explored the possibility to substitute PTFE with either polyvinyl-alcohol (PVA) or PlastiDip (CPD; i.e. synthetic rubber) as binder for AC-based cathode in SSM-MFC. Sintered activated carbon (SAC) was also tested due to its ease of manufacturing and the fact that no stainless steel collector is needed. Results indicate that the SSM-MFC having PTFE cathodes were the most powerful measuring 1617 μW (11 W·m−3 or 101 mW·m−2). SSM-MFC with PVA and CPD as binders were producing on average the same level of power (1226 ± 90 μW), which was 24% less than the SSM-MFC having PTFE-based cathodes. When balancing the power by the cost and environmental impact, results clearly show that PVA was the best alternative. Power wise, the SAC cathodes were shown being the less performing (≈1070 μW). Nonetheless, the lower power of SAC was balanced by its inexpensiveness. Overall results indicate that (i) PTFE is yet the best binder to employ, and (ii) SAC and PVA-based cathodes are promising alternatives that would benefit from further improvements

Self-stratifying microbial fuel cell: The importance of the cathode electrode immersion height

© 2018 The Author(s) Power generation of bioelectrochemical systems (BESs) is a very important electrochemical parameter to consider particularly when the output has to be harvested for practical applications. This work studies the effect of cathode immersion on the performance of a self-stratified membraneless microbial fuel cell (SSM-MFC) fuelled with human urine. Four different electrolyte immersion heights, i.e. 1/4, 2/4, 3/4 and fully submerged were considered. The SSM-MFC performance improved with increased immersion up to 3/4. The output dropped drastically when the cathode was fully submerged with the conditions becoming fully anaerobic. SSM-MFC with 3/4 submerged cathode had a maximum power output of 3.0 mW followed by 2.4 mW, 2.0 mW, and 0.2 mW for the 2/4, 1/4 and fully submerged conditions. Durability tests were run on the best performing SSM-MFC with 3/4 cathode immersed and showed an additional increase in the electrochemical output by 17% from 3.0 mW to 3.5 mW. The analysis performed on the anode and cathode separately demonstrated the stability in the cathode behaviour and in parallel an improvement in the anodic performance during one month of investigation

Scalability of self-stratifying microbial fuel cell: Towards height miniaturisation

© 2019 The Authors The scalability of bioelectrochemical systems is a key parameter for their practical implementation in the real-world. Up until now, only urine-fed self-stratifying microbial fuel cells (SSM-MFCs) have been shown to be scalable in width and length with limited power density losses. For practical reasons, the present work focuses on the scalability of SSM-MFCs in the one dimension that has not yet been investigated, namely height. Three different height conditions were considered (1 cm, 2 cm and 3 cm tall electrodes). The normalised power density of the 2 cm and 3 cm conditions were similar either during the durability test under a hydraulic retention time of ≈39 h (i.e. 15.74 ± 0.99 μW.cm −3 ) and during the polarisation experiments (i.e. 27.79 ± 0.92 μW.cm −3 ). Conversely, the 1 cm condition had lower power densities of 11.23 ± 0.07 μW.cm −3 and 17.73 ± 3.94 μW.cm −3 both during the durability test and the polarisation experiment, respectively. These results confirm that SSM-MFCs can be scaled in all 3 dimensions with minimal power density losses, with a minimum height threshold for the electrode comprised between 1 cm and 2 cm

Scalability and stacking of self-stratifying microbial fuel cells treating urine

The scalability of Microbial fuel cells (MFCs) is key to the development of stacks. A recent study has shown that self-stratifying membraneless MFCs (S-MFCs) could be scaled down to 2 cm without performance deterioration. However, the scaling-up limit of S-MFC is yet unknown. Here the study evaluates the scale-up height of S-MFCs treating urine, from 2 cm, 4 cm to 12 cm high electrodes. The electrochemical properties of the S-MFCs were investigated after steady-states were established, following a 70-days longevity study. The electrochemical properties of the 2 cm and 4 cm conditions were similar (5.45 ± 0.32 mW per cascade). Conversely, the 12 cm conditions had much lower power output (1.48 ± 0.15 mW). The biofilm on the 12 cm cathodes only developed on the upper 5–6 cm of the immersed part of the electrode suggesting that the cathodic reactions were the limiting factor. This hypothesis was confirmed by the cathode polarisations showing that the 12 cm S-MFC had low current density (1.64 ± 9.53 µA cm−2, at 0 mV) compared to the other two conditions taht had similar current densities (192.73 ± 20.35 µA cm−2, at 0 mV). These results indicate that S-MFC treating urine can only be scaled-up to an electrode height of around 5–6 cm before the performance is negatively affected

PEE POWER® urinal II - Urinal scale-up with microbial fuel cell scale-down for improved lighting

© 2018 The Authors A novel design of microbial fuel cells (MFC) fuelled with undiluted urine was demonstrated to be an efficient power source for decentralised areas, but had only been tested under controlled laboratory conditions. Hence, a field-trial was carried out to assess its feasibility for practical implementation: a bespoke stack of 12 MFC modules was implemented as a self-sufficient lit urinal system at UK's largest music festival. Laboratory investigation showed that with a hydraulic retention time (HRT) of 44 h, a cascade of 4 modules (19.2 L displacement volume) was continuously producing ≈150 mW. At the same HRT, the chemical oxygen demand (COD) was reduced from 5586 mg COD·L−1 to 625 mg COD·L−1. Field results of the system under uncontrolled usage indicate an optimal retention time for power production between 2h30 and ≈9 h. When measured (HRT of ≈11h40), the COD decreased by 48% and the total nitrogen content by 13%. Compared to the previous PEE POWER® field-trial (2015), the present system achieved a 37% higher COD removal with half the HRT. The 2016 set-up produced ≈30% more energy in a third of the total volumetric footprint (max 600 mW). This performance corresponds to ≈7-fold technological improvement

Self-stratified and self-powered micro-supercapacitor integrated into a microbial fuel cell operating in human urine

© 2019 The Authors A self-stratified microbial fuel cell fed with human urine with a total internal volume of 0.55 ml was investigated as an internal supercapacitor, for the first time. The internal self-stratification allowed the development of two zones within the cell volume. The oxidation reaction occurred on the bottom electrode (anode) and the reduction reaction on the top electrode (cathode). The electrodes were discharged galvanostatically at different currents and the two electrodes were able to recover their initial voltage value due to their red-ox reactions. Anode and cathode apparent capacitance was increased after introducing high surface area activated carbon embedded within the electrodes. Peak power produced was 1.20 ± 0.04 mW (2.19 ± 0.06 mW ml −1 ) for a pulse time of 0.01 s that decreased to 0.65 ± 0.02 mW (1.18 ± 0.04 mW ml −1 ) for longer pulse periods (5 s). Durability tests were conducted over 44 h with ≈2600 discharge/recharge cycles. In this relatively long-term test, the equivalent series resistance increased only by 10% and the apparent capacitance decreased by 18%

Air-breathing cathode self-powered supercapacitive microbial fuel cell with human urine as electrolyte

In this work, a membraneless microbial fuel cell (MFC) with an empty volume of 1.5 mL, fed continuously with hydrolysed urine, was tested in supercapacitive mode (SC-MFC). In order to enhance the power output, a double strategy was used: i) a double cathode was added leading to a decrease in the equivalent series resistance (ESR); ii) the apparent capacitance was boosted up by adding capacitive features on the anode electrode. Galvanostatic (GLV) discharges were performed at different discharge currents. The results showed that both strategies were successful obtaining a maximum power output of 1.59 ± 0.01 mW (1.06 ± 0.01 mW mL−1) at pulse time of 0.01 s and 0.57 ± 0.01 mW (0.38 ± 0.01 mW mL−1) at pulse time of 2 s. The highest energy delivered at ipulse equal to 2 mA was 3.3 ± 0.1 mJ. The best performing SC-MFCs were then connected in series and parallel and tested through GLV discharges. As the power output was similar, the connection in parallel allowed to roughly doubling the current produced. Durability tests over ≈5.6 days showed certain stability despite a light overall decrease

On hybrid circuits exploiting thermistive properties of slime mould

Slime mould Physarum polycephalum is a single cell visible by the unaided eye. Let the slime mould span two electrodes with a single protoplasmic tube: if the tube is heated to approximately ≈40 °C, the electrical resistance of the protoplasmic tube increases from ≈3 Mω to ≈10,000 Mω. The organisms resistance is not proportional nor correlated to the temperature of its environment. Slime mould can therefore not be considered as a thermistor but rather as a thermic switch. We employ the P. polycephalum thermic switch to prototype hybrid electrical analog summator, NAND gates, and cascade the gates into Flip-Flop latch. Computing operations performed on this bio-hybrid computing circuitry feature high repeatability, reproducibility and comparably low propagation delays

Impact of disinfectant on the electrical outputs of urine-fed ceramic and membrane-less microbial fuel cell cascades

The application of microbial fuel cells in sanitation has demonstrated feasibility in supplying electricity and providing safety in underserved communities, especially at toilet blocks. Two different designs of urine fed MFC cascades, ceramic MFCs (c-MFC) and self-stratifying MFCs (s-MFC), have been employed in large-scale feasibility studies. As part of a pre-commercialisation approach, this study verified the resilience of each design when a commercial disinfectant was introduced into the system. Five different conditions, varying in concentrations (24.2 mM–604.5 mM) and the total volume (50–500 mL) of sodium hypochlorite disinfectant introduced, were tested. Upon adding the disinfectant, both types of MFC-cascades exhibited rapid power drops with response times lower than 5 min in all tested conditions, followed by relatively swift recovery times of up to 250 min. The volume of disinfectant introduced had a greater impact on power output than its concentration or dose. Comparing the two designs, the c-MFC demonstrated a much larger voltage drop, up to 0 mV, and shorter recovery time compared to the s-MFC under most test conditions, mainly attributed to the presence (c-MFC) or absence (s-MFC) of a membrane. Overall, both types of MFCs exhibited strong resilience to sodium hypochlorite additions, thereby highlighting the commercial potential of the technology towards safe off-grid sanitation

Microbial fuel cells directly powering a microcomputer

© 2019 The Authors Many studies have demonstrated that microbial fuel cells (MFC) can be energy-positive systems and power various low power applications. However, to be employed as a low-level power source, MFC systems rely on energy management circuitry, used to increase voltage levels and act as energy buffers, thus delivering stable power outputs. But stability comes at a cost, one that needs to be kept minimal for the technology to be deployed into society. The present study reports, for the first time, the use of a MFC system that directly and continuously powered a small application without any electronic intermediary. A cascade comprising four membrane-less MFCs modules and producing an average of 62 mA at 2550 mV (158 mW) was used to directly power a microcomputer and its screen (Gameboy Color, Nintendo®). The polarisation experiment showed that the cascade produced 164 mA, at the minimum voltage required to run the microcomputer (ca. 1.850 V). As the microcomputer only needed ≈70 mA, the cascade ran at a higher voltage (2.550 V), thus, maintaining the individual modules at a high potential (>0.55 V). Running the system at these high potentials helped avoid cell reversal, thus delivering a stable level of energy without the support of any electronics