Influence of mixed culture system on the growth performance of Aspergillus oryzae and Saccharomyces cerevisiae

This study describes a novel strategy to improve the α-galactosidase and invertase production of Saccharomyces cerevisiae by co-cultivating it with Aspergillus oryzae. In the mixed culture, the growth of the both strains was repressed, and the protein synthesis for the yeast cell wall was promoted significantly. As a result, α-galactosidase and invertase activities of the mixed culture reached 85.16 and 561.60 Uml-1, over 9 and 15 fold greater than the values obtained in the cultures of single strain, respectively. During the simultaneous saccharification and fermentation procedure, ethanol with the concentration of 15.2% (v/v) was obtained from soybean sirup in 60 h at 30°C, and the conversion efficiency of total sugar to ethanol was 96.3% of the theoretical ethanol yield.Keywords: Co-cultivation, Aspergillus oryzae, Saccharomyces cerevisiae, α-galactosidase, invertaseAfrican Journal of Biotechnology Vol. 12(21), pp. 3272-327

Synthesis and characterization of interspecific trigenomic hybrids and allohexaploids between three cultivated Brassica allotetraploids and wild species Brassica fruticulosa

Interspecific reciprocal crosses between three cultivated Brassica allotetraploids and one wild species Brassica fruticulosa (FF, 2n =16) were made and the trigenomic hybrids were produced only with embryo rescue. From the crosses with Brassica juncea (AABB, 2n = 36) and Brassica napus (AACC, 2n = 38), hybrids (F.AB, 2n = 26; F.AC, 2n = 27) were obtained only with B. fruticulosa as female, but the reciprocal crosses with Brassica carinata (BBCC, 2n = 34) gave rise to hybrids (F.BC/BC.F, 2n = 25). These hybrids showed an intermediate morphology and were sterile for male and female except those with B. carinata. All the triploids showed high frequency of bivalents during diakinesis and metaphase I (MI) of meiosis. The allohexaploids (FF.AABB, 2n = 52; FF.AACC, 2n = 54; BBCC.FF, 2n = 50) were synthesized by colchicine treatments of respective hybrids in vitro, which showed growth vigor and had larger stature than the hybrids, but were male sterile except one (FF.AABB). They produced progenies with the expected chromosome complements (F.AABB, 2n = 44; F.AACC, 2n = 46; BBCC.F, 2n = 42) after pollination by respective Brassica allotetraploids. In these allohexaploids, chromosomes were mainly paired as bivalents at diakinesis and segregated equally at anaphase I (AI) during meiosis of pollen mother cells (PMCs). These allohexaploids and progenies were valuable for the breeding of Brassica crops.Key words: Brassica allotetraploids, Brassica fruticulosa, interspecific hybrids, crossability, morphology, genomic affinity

A scheme for demodulation of fiber grating sensors with high resolution

2000-2001 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Performance analysis of finned-tube CO2 gas cooler with advanced 1D-3D CFD modelling development and simulation

Research data for this article: Data not available / Data will be made available on request.Supplementary material is available online at: https://www.sciencedirect.com/science/article/pii/S1359431120307122?via%3Dihub#s0070 .Due to natural refrigerant applied, CO2 transcritical refrigeration and heat pump systems have been widely applied and attracted more attentions. As a main component, CO2 gas cooler plays an important role in the system performance and thus requires further development for design and control optimizations with advanced technology. Correspondingly, a new coupled 1D and 3D Computational Fluid Dynamics (CFD) model on a finned-tube CO2 gas cooler has been proposed and developed. The CFD model has been validated by comparing with literatures for parameters including airside heat transfer coefficient, refrigerant side temperature profile as well as heating capacity. The model has been applied to predict the heat exchanger performance at different operating conditions of both air and refrigerant sides and maldistributions of air flow inlet. It is found from the simulation results that the refrigerant temperature decreases abruptly in the first coil row and the refrigerant temperature profile along the heat exchanger tubes is affected by thermal conduction between two adjacent tube rows through fins. In addition, the higher air flow inlet velocity can reduce greatly the coil approach temperature and thus improve the system efficiency. Similar effect can also be found from refrigerant pressure. Furthermore, the non-uniform air flow patterns can affect considerably the coil performance in terms of the refrigerant temperature profile, coil approach temperature, coil heating capacity and system energy efficiency. The developed CFD model can be an efficient tool for the performance evaluation and optimisation of the CO2 gas cooler and its associated system.The authors would like to acknowledge the support received from Research Councils UK (ESPRC, EP/R000298/1) for this research project

Effect of annealing on the performance of CrO3/ZnO light emitting diodes

Heterojunction CrO3/ZnO light emitting diodes have been fabricated. Their performance was investigated for different annealing temperature for ZnO nanorods. Annealing in oxygen atmosphere had significant influence on carrier concentration in the nanorods, as well as on the emission spectra of the nanorods. Surprisingly, annealing conditions, which yield the lowest band edge-to-defect emission ratio in the photoluminescence spectra, result in the highest band edge-to-defect emission ratio in the electroluminescence spectra. The influence of the native defects on ZnO light emitting diode performance is discussed. © 2009 American Institute of Physics.published_or_final_versio

Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization

Calreticulin (CRT) is a highly conserved and ubiquitously expressed Ca2+-binding protein in multicellular eukaryotes. As an endoplasmic reticulum-resident protein, CRT plays a key role in many cellular processes including Ca2+ storage and release, protein synthesis, and molecular chaperoning in both animals and plants. CRT has long been suggested to play a role in plant sexual reproduction. To begin to address this possibility, we cloned and characterized the full-length cDNA of a new CRT gene (PhCRT) from Petunia. The deduced amino acid sequence of PhCRT shares homology with other known plant CRTs, and phylogenetic analysis indicates that the PhCRT cDNA clone belongs to the CRT1/CRT2 subclass. Northern blot analysis and fluorescent in situ hybridization were used to assess PhCRT gene expression in different parts of the pistil before pollination, during subsequent stages of the progamic phase, and at fertilization. The highest level of PhCRT mRNA was detected in the stigma–style part of the unpollinated pistil 1 day before anthesis and during the early stage of the progamic phase, when pollen is germinated and tubes outgrow on the stigma. In the ovary, PhCRT mRNA was most abundant after pollination and reached maximum at the late stage of the progamic phase, when pollen tubes grow into the ovules and fertilization occurs. PhCRT mRNA transcripts were seen to accumulate predominantly in transmitting tract cells of maturing and receptive stigma, in germinated pollen/growing tubes, and at the micropylar region of the ovule, where the female gametophyte is located. From these results, we suggest that PhCRT gene expression is up-regulated during secretory activity of the pistil transmitting tract cells, pollen germination and outgrowth of the tubes, and then during gamete fusion and early embryogenesis

High energy emission from microquasars

The microquasar phenomenon is associated with the production of jets by X-ray binaries and, as such, may be associated with the majority of such systems. In this chapter we briefly outline the associations, definite, probable, possible, and speculative, between such jets and X-ray, gamma-ray and particle emission.Comment: Contributing chapter to the book Cosmic Gamma-Ray Sources, K.S. Cheng and G.E. Romero (eds.), to be published by Kluwer Academic Publishers, Dordrecht, 2004. (19 pages

Identification of Extracellular Segments by Mass Spectrometry Improves Topology Prediction of Transmembrane Proteins

Transmembrane proteins play crucial role in signaling, ion transport, nutrient uptake, as well as in maintaining the dynamic equilibrium between the internal and external environment of cells. Despite their important biological functions and abundance, less than 2% of all determined structures are transmembrane proteins. Given the persisting technical difficulties associated with high resolution structure determination of transmembrane proteins, additional methods, including computational and experimental techniques remain vital in promoting our understanding of their topologies, 3D structures, functions and interactions. Here we report a method for the high-throughput determination of extracellular segments of transmembrane proteins based on the identification of surface labeled and biotin captured peptide fragments by LC/MS/MS. We show that reliable identification of extracellular protein segments increases the accuracy and reliability of existing topology prediction algorithms. Using the experimental topology data as constraints, our improved prediction tool provides accurate and reliable topology models for hundreds of human transmembrane proteins

Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats

The association of IGF-I gene polymorphisms with certain traits in 708 individuals of two Chinese dairy-goat breeds (Guanzhong and Xinong Saanen) was investigated. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods were employed in screening for genetic variation. Two novel mutations were detected in the 5'-flanking region and in intron 4 of IGF-I gene, viz., g.1617 G > A and g.5752 G > C (accession D26119.2), respectively. The associations of the g.1617 G > A mutation with milk yield and the body size were not significant (p > 0.05). However, in the case of g.5752 G > C, Xinong Saanen dairy goats with the CG genotype presented longer bodies (p < 0.05). Chest circumference (p < 0.05) was larger in Guanzhong goats with the GG genotype. In Xinong Saanen dairy goats with the CC genotype, milk yields were significantly higher during the first and second lactations (p < 0.05). Hence, the g.5752 G > C mutation could facilitate association analysis and serve as a genetic marker for Chinese dairy-goat breeding and genetics