40,748 research outputs found

    Electronic structure of heavily electron-doped BaFe1.7_{1.7}Co0.3_{0.3}As2_2 studied by angle-resolved photoemission

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    We have performed high-resolution angle-resolved photoemission spectroscopy on heavily electron-doped non-superconducting (SC) BaFe1.7_{1.7}Co0.3_{0.3}As2_2. We find that the two hole Fermi surface pockets at the zone center observed in the hole-doped superconducting Ba0.6_{0.6}K0.4_{0.4}Fe2_2As2_2 are absent or very small in this compound, while the two electron pockets at the M point significantly expand due to electron doping by the Co substitution. Comparison of the Fermi surface between non-SC and SC samples indicates that the coexistence of hole and electron pockets connected via the antiferromagnetic wave vector is essential in realizing the mechanism of superconductivity in the iron-based superconductors.Comment: 5 pages, 4 figure

    A novel and convenient method to immunize animals: Inclusion bodies from recombinant bacteria as antigen to directly immunize animals

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    Purified recombinant protein has been routinely used to immunize rabbits to produce polyclonal antibodies. The process of the purification of recombinant proteins from bacterial inclusion bodies is both labor intensive and time consuming. To determine whether whole inclusion bodies can be used as antigen in polyclonal antibody production, we amplified Gallus gallus phospholipase A2 gene by RTPCR, fused with glutathione-S-transferase (GST), and expressed recombinant Escherichia coli strain Rosseta (DE3) in the form of inclusion bodies. The inclusion bodies isolated from the bacteria cells were directly injected into rabbits and hens at 300 μg dose four times. Western blot was performed to detect antibodies in serum and in egg yolk, which revealed strong immunoreactions. The results suggested that using recombinant protein-containing inclusion bodies as antigen is a novel and convenient method to immunize animals for polyclonal antibody production.Key words: Polyclonal antibodies, inclusion bodies, antigen, recombinant phospholipase A2

    Deformation monitoring of high-latitude permafrost region of northeastern China with time series inSAR technique

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    Abstract. Permafrost distributed in northeast China is the only high-altitude permafrost in China. The deformation monitoring over this permafrost region is of great importance to local climate change and ecological environments. This study focuses on the deformation monitoring of high-latitude permafrost in northeast China with time series InSAR technique. The spatial distribution characteristics, the annual deformation rates and the temporal deformation evolutions of permafrost could be retrieved from multi-temporal InSAR processing with Sentinel-1 TOPS datasets. This work concludes that time series InSAR technique could help to retrieve a comprehensive and reliable permafrost deformation, while a long time-series of displacements facilitated to better understand permafrost kinematics.</p

    A comparison of the local spiral structure from Gaia DR2 and VLBI maser parallaxes

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    Context. The Gaia mission has released the second data set (Gaia DR2), which contains parallaxes and proper motions for a large number of massive, young stars. Aims. We investigate the spiral structure in the solar neighborhood revealed by Gaia DR2 and compare it with that depicted by VLBI maser parallaxes. Methods. We examined three samples with different constraints on parallax uncertainty and distance errors and stellar spectral types: (1) all OB stars with parallax errors of less than 10%; (2) only O-type stars with 0.1 mas errors imposed and with parallax distance errors of less than 0.2 kpc; and (3) only O-type stars with 0.05 mas errors imposed and with parallax distance errors of less than 0.3 kpc. Results. In spite of the significant distance uncertainties for stars in DR2 beyond 1.4 kpc, the spiral structure in the solar neighborhood demonstrated by Gaia agrees well with that illustrated by VLBI maser results. The O-type stars available from DR2 extend the spiral arm models determined from VLBI maser parallaxes into the fourth Galactic quadrant, and suggest the existence of a new spur between the Local and Sagittarius arms.Comment: 4 pages, 3 figures, 1 table, accepted for publication in A&

    Lycopene protects against hypoxia/reoxygenation-induced apoptosis by preventing mitochondrial dysfunction in primary neonatal mouse cardiomyocytes

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    BACKGROUND: Hypoxia/reoxygenation(H/R)-induced apoptosis of cardiomyocytes plays an important role in myocardial injury. Lycopene is a potent antioxidant carotenoid that has been shown to have protective properties on cardiovascular system. The aim of the present study is to investigate the potential for lycopene to protect the cardiomyocytes exposed to H/R. Moreover, the effect on mitochondrial function upon lycopene exposure was assessed. METHODS AND FINDINGS: Primary cardiomyocytes were isolated from neonatal mouse and established an in vitro model of H/R which resembles ischemia/reperfusion in vivo. The pretreatment of cardiomyocytes with 5 microM lycopene significantly reduced the extent of apoptosis detected by TUNEL assays. To further study the mechanism underlying the benefits of lycopene, interactions between lycopene and the process of mitochondria-mediated apoptosis were examined. Lycopene pretreatment of cardiomyocytes suppressed the activation of the mitochondrial permeability transition pore (mPTP) by reducing the intracellular reactive oxygen species (ROS) levels and inhibiting the increase of malondialdehyde (MDA) levels caused by H/R. Moreover, the loss of mitochondrial membrane potential, a decline in cellular ATP levels, a reduction in the amount of cytochrome c translocated to the cytoplasm and caspase-3 activation were observed in lycopene-treated cultures. CONCLUSION: The present results suggested that lycopene possesses great pharmacological potential in protecting against H/R-induced apoptosis. Importantly, the protective effects of lycopene may be attributed to its roles in improving mitochondrial function in H/R-treated cardiomyocytes.published_or_final_versio

    Magnetic impurities in the two-band s±s_\pm-wave superconductors

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    We investigate the effects of magnetic impurities in a superconducting state with s±s_\pm pairing symmetry. Within a two-band model, we find that the intra-band magnetic scattering serves as a pair breaker while the inter-band magnetic scattering preserves pairing and hardly affects transition temperature in the Born limit. We also show that the same physics can persist beyond the weak scattering region. Our results coincide with recent experimental measurements in iron-based superconductors and thus provides an indirect evidence of the possible s±s_\pm pairing symmetry in these materials.Comment: 5 pages,4 figure

    Superconductivity induced by Ni doping in BaFe2_2As2_2

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    A series of 122 phase BaFe2−x_{2-x}Nix_xAs2_2 (xx = 0, 0.055, 0.096, 0.18, 0.23) single crystals were grown by self flux method and a dome-like Ni doping dependence of superconducting transition temperature is discovered. The transition temperature TconT_c^{on} reaches a maximum of 20.5 K at xx = 0.096, and it drops to below 4 K as xx ≥\geq 0.23. The negative thermopower in the normal state indicates that electron-like charge carrier indeed dominates in this system. This Ni-doped system provides another example of superconductivity induced by electron doping in the 122 phase.Comment: 7 pages, 5 figures, revised version, added EDX result, accepted for special issue of NJ
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