26 research outputs found

    P2X7 receptor contributes to long-term neuroinflammation and cognitive impairment in sepsis-surviving mice

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    Introduction: sepsis is defined as a multifactorial debilitating condition with high risks of death. The intense inflammatory response causes deleterious effects on the brain, a condition called sepsis-associated encephalopathy. Neuroinflammation or pathogen recognition are able to stress cells, resulting in ATP (Adenosine Triphosphate) release and P2X7 receptor activation, which is abundantly expressed in the brain. The P2X7 receptor contributes to chronic neurodegenerative and neuroinflammatory diseases; however, its function in long-term neurological impairment caused by sepsis remains unclear. Therefore, we sought to evaluate the effects of P2X7 receptor activation in neuroinflammatory and behavioral changes in sepsis-surviving mice. Methods: sepsis was induced in wild-type (WT), P2X7−/− , and BBG (Brilliant Blue G)-treated mice by cecal ligation and perforation (CLP). On the thirteenth day after the surgery, the cognitive function of mice was assessed using the novel recognition object and Water T-maze tests. Acetylcholinesterase (AChE) activity, microglial and astrocytic activation markers, and cytokine production were also evaluated. Results: Initially, we observed that both WT and P2X7−/− sepsis-surviving mice showed memory impairment 13 days after surgery, once they did not differentiate between novel and familiar objects. Both groups of animals presented increased AChE activity in the hippocampus and cerebral cortex. However, the absence of P2X7 prevented partly this increase in the cerebral cortex. Likewise, P2X7 absence decreased ionized calcium-binding protein 1 (Iba−1 ) and glial fibrillary acidic protein (GFAP) upregulation in the cerebral cortex of sepsis-surviving animals. There was an increase in GFAP protein levels in the cerebral cortex but not in the hippocampus of both WT and P2X7−/− sepsis-surviving animals. Pharmacological inhibition or genetic deletion of P2X7 receptor attenuated the production of Interleukin-1ÎČ (IL-1ÎČ), Tumor necrosis factor-α (TNF-α), and Interleukin-10 (IL-10). Conclusion: the modulation of the P2X7 receptor in sepsis-surviving animals may reduce neuroinflammation and prevent cognitive impairment due to sepsisassociated encephalopathy, being considered an important therapeutic target

    The GALAH Survey: Chemical tagging and chrono-chemodynamics of accreted halo stars with GALAH+ DR3 and GaiaGaia eDR3

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    © 2021 The Author(s) Published by Oxford University Press on behalf of Royal Astronomical Society. This is the accepted manuscript version of an article which has been published in final form at https://doi.org/10.1093/mnras/stab3504Since the advent of GaiaGaia astrometry, it is possible to identify massive accreted systems within the Galaxy through their unique dynamical signatures. One such system, GaiaGaia-Sausage-Enceladus (GSE), appears to be an early "building block" given its virial mass >1010 M⊙> 10^{10}\,\mathrm{M_\odot} at infall (z∌1−3z\sim1-3). In order to separate the progenitor population from the background stars, we investigate its chemical properties with up to 30 element abundances from the GALAH+ Survey Data Release 3 (DR3). To inform our choice of elements for purely chemically selecting accreted stars, we analyse 4164 stars with low-α\alpha abundances and halo kinematics. These are most different to the Milky Way stars for abundances of Mg, Si, Na, Al, Mn, Fe, Ni, and Cu. Based on the significance of abundance differences and detection rates, we apply Gaussian mixture models to various element abundance combinations. We find the most populated and least contaminated component, which we confirm to represent GSE, contains 1049 stars selected via [Na/Fe] vs. [Mg/Mn] in GALAH+ DR3. We provide tables of our selections and report the chrono-chemodynamical properties (age, chemistry, and dynamics). Through a previously reported clean dynamical selection of GSE stars, including 30<JR / kpc km s−1<5530 < \sqrt{J_R~/~\mathrm{kpc\,km\,s^{-1}}} < 55, we can characterise an unprecedented 24 abundances of this structure with GALAH+ DR3. Our chemical selection allows us to prevent circular reasoning and characterise the dynamical properties of the GSE, for example mean JR / kpc km s−1=26−14+9\sqrt{J_R~/~\mathrm{kpc\,km\,s^{-1}}} = 26_{-14}^{+9}. We find only (29±1)%(29\pm1)\% of the GSE stars within the clean dynamical selection region. Our methodology will improve future studies of accreted structures and their importance for the formation of the Milky Way.Peer reviewedFinal Accepted Versio

    The Milky Way Tomography with SDSS: I. Stellar Number Density Distribution

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    Abridged: We estimate the distances to ~48 million stars detected by the Sloan Digital Sky Survey and map their 3D number density distribution in 100 < D < 20 kpc range over 6,500 deg^2 of sky. The data show strong evidence for a Galaxy consisting of an oblate halo, a disk component, and a number of localized overdensities with exponential disk parameters (bias-corrected for an assumed 35% binary fraction) H_1 = 300 pc, L_1 = 2600 pc, H_2 = 900 pc, L_2 = 3600 pc, and local density normalization of 12%. We find the halo to be oblate, with best-fit axis ratio c/a = 0.64, r^{-2.8} profile, and the local halo-to-thin disk normalization of 0.5%. We estimate the errors of derived model parameters to be no larger than ~20% (disk scales) and ~10% (thick disk normalization). While generally consistent with the above model, the density distribution shows a number of statistically significant localized deviations. We detect two overdensities in the thick disk region at (R, Z) ~ (6.5, 1.5)kpc and (R, Z) ~ (9.5, 0.8) kpc, and a remarkable density enhancement in the halo covering >1000deg^2 of sky towards the constellation of Virgo, at distances of ~6-20 kpc. Compared to a region symmetric with respect to the l=0 line, the Virgo overdensity is responsible for a factor of 2 number density excess and may be a nearby tidal stream or a low-surface brightness dwarf galaxy merging with the Milky Way. After removal of the resolved overdensities, the remaining data are consistent with a smooth density distribution; we detect no evidence of further unresolved clumpy substructure at scales ranging from ~50pc in the disk, to ~1 - 2 kpc in the halo.Comment: 60 pages, 46 figures (reduced resolution; see the ApJ for hi-res version

    The Milky Way Tomography with SDSS: II. Stellar Metallicity

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    Using effective temperature and metallicity derived from SDSS spectra for ~60,000 F and G type main sequence stars (0.2<g-r<0.6), we develop polynomial models for estimating these parameters from the SDSS u-g and g-r colors. We apply this method to SDSS photometric data for about 2 million F/G stars and measure the unbiased metallicity distribution for a complete volume-limited sample of stars at distances between 500 pc and 8 kpc. The metallicity distribution can be exquisitely modeled using two components with a spatially varying number ratio, that correspond to disk and halo. The two components also possess the kinematics expected for disk and halo stars. The metallicity of the halo component is spatially invariant, while the median disk metallicity smoothly decreases with distance from the Galactic plane from -0.6 at 500 pc to -0.8 beyond several kpc. The absence of a correlation between metallicity and kinematics for disk stars is in a conflict with the traditional decomposition in terms of thin and thick disks. We detect coherent substructures in the kinematics--metallicity space, such as the Monoceros stream, which rotates faster than the LSR, and has a median metallicity of [Fe/H]=-0.96, with an rms scatter of only ~0.15 dex. We extrapolate our results to the performance expected from the Large Synoptic Survey Telescope (LSST) and estimate that the LSST will obtain metallicity measurements accurate to 0.2 dex or better, with proper motion measurements accurate to ~0.2 mas/yr, for about 200 million F/G dwarf stars within a distance limit of ~100 kpc (g<23.5). [abridged]Comment: 40 pages, 21 figures, emulateApJ style, accepted to ApJ, high resolution figures are available from http://www.astro.washington.edu/ivezic/sdss/mw/astroph0804.385

    Colonising her cultural identity : geographies of a hybrid identity in colonial Turanga : a thesis presented to Massey University, Palmerston North, New Zealand in fulfilment of the requirements for the degree of Master of Arts in Geography

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    This thesis is a feminist historical geography of the construction of a woman's identity in Turanga (now known as Gisborne), New Zealand from 1840 to 1893. Using textual analysis of private and public documents from the time period, it reveals socio-spatial power structures, cultural relations, and gender relations of the place and time, and the ways in which they contribute to the conditions necessary for a fluid cultural identity. The research finds that three key conditions of cultural hybridity proposed by Homi K. Bhabha are applicable to a subject of the place and time period, Keita Wyllie. The thesis further argues that a fourth condition is necessary, namely the role of space as an active and constituent agent in the process of identity formation. The research complements previous histories of the East Coast/Turanga region by examining issues of cross-cultural identity, power structures and gender relations previously absent from historical accounts of the area

    Probing Halos of Galaxies at Very Large Radii Using Background QSOs.

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    Gaseous halos of nine nearby galaxies (with redshifts cz < 6000 km s-1) were probed at large galactocentric radii using background quasars observed with the Hubble Space Telescope Goddard High Resolution Spectrograph and the Space Telescope Imaging Spectrograph. The projected quasar-galaxy separations range from 55 to 387 h75-1 kpc. Lyα absorption lines were successfully detected in the spectra of five quasars, at impact parameters of up to ∌170 h75-1 kpc from the center of the nearby galaxy, and in each case at wavelengths consistent with the galaxy's redshift. Our observations include the lowest redshift Lyα lines detected to date. H I velocity fields were obtained at the Very Large Array for three of the galaxies in our sample (in one case the velocity field was available from the literature) to derive their rotation curves. When comparing the inner rotation curves of the galaxies with the velocity at large radius provided by the Lyα line, it is apparent that it is very difficult to explain the observed Lyα velocity as due to gas in an extended rotating disk. In most cases, one would need to invoke large warps in the outer gas disks and also thick gas disks to reconcile the observed velocities with the predicted ones. Indeed, in one case, the Lyα line velocity indicates, in fact, counterrotation with respect to the inner disk rotation. In light of these results, we conclude that in a typical galaxy there is no longer detectable atomic gas corotating in an extended disk at radii greater than 35α-1, where α-1 is the stellar disk exponential scale length. The cosmic web is the most likely origin for the detected Lyα lines. Our observations confirm the recent Bowen et al. correlation of equivalent widths with the local volume density of galaxies around the sight line, and the observed equivalent widths of the lines are consistent with expectations of the cosmic web

    Feminist historical geographies: doing and being

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    As part of GPC’s 25-year anniversary celebrations, this article explores possibilities and prospects for feminist historical geographies and geographers. Here I define feminist historical geography as scholarship which asks geographical questions of historical material and is informed by feminist theories, approaches and methodologies. Its empirical subject matter is necessarily expansive and diverse, but often has a particular focus on the lives of women and other marginalized groups, and on the ways gender and space were co-constituted. This essay interrogates recent developments within this broad terrain, specifically articles and books published in the period from around 2000 onwards and either appearing in geography journals or written by those self-identifying as geographers. The main exception is work by historians and archaeologists interested in gender, space and place, which is cited here in an attempt to open up new research directions for feminist historical geographers. In what follows, we shuttle across spaces and between scales, roaming from the sites of empire to the intimate geographies of the home, from landscapes and buildings to personal possessions like clothes and letters. Doing so is a deliberate act intended both to demonstrate the liveliness of feminist historical geographies broadly conceived and to counter hierarchical readings of space, society and history with their inherent danger of privileging the public over the private, and the exceptional over the everyday and mundane
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