Elf: computer automation and error correction for a microwave network analyzer

A microwave measurement system has been developed that combines a personal computer (PC) and an conventional vector network analyzer to yield a full complex-error-corrected automatic network analyzer. The system consists of a Hewlett-Packard HP 8410C network analyzer, an HP 8350B sweep oscillator, and an IBM PC. A program called Elf runs on the PC, performing calibration and measurement algorithms and providing a flexible, menu-oriented user interface. The system, when calibrated, achieves a worst-case measurement error vector of magnitude ≤ 0.02 for transmission and reflection coefficient measurements over the 2-12.4-GHz frequency range and has a measurement speed of three frequency points/s. Elf provides an inexpensive method for upgrading the HP 8410 to achieve the high accuracy of an automatic network analyzer

Puff, an Interactive Microwave Computer Aided Design Program for Personal Computers

We will demonstrate a CAD program designed for the lay out and analysis of microstrip circuits. The program runs on the IBM PC or AT. Circuit elements are selected from a parts list and drawn on the screen using the cursor keys. The analysis may then be performed, directly from the screen drawing. Puff has been used by microwave students in classes at the California Institute of Technology and the University of California at Los Angeles

The determination of the elastic modulus of rubber mooring tethers and their use in coastal moorings

Compliance must be supplied to any surface mooring to allow the buoy to move with the waves and currents, and remain moored in position. This can be supplied with a traditional chain catenary or newer compliant elastic tether or stretch hose technologies. Some applications of each of these three techniques are shown, with the emphasis placed on the use of compliant elastic tethers. For modeling and designing these moorings, the elastic modulus of the tether material must be known. Therefore, a new and used piece of elastic material was terminated, tested for the stretch-strain relationship under set conditions, and the elastic modulus calculated. For these tests, the elastic tether was stretched out to a mean elongation between 100 and 250%, then cycled about that stretch by ±25 and ±50% to duplicate a moored application. The resultant elastic modulus is presented to aid in mooring design. At low elongations, the elastic modulus is constant at about 125 PSI, but as the mean elongation increases the modulus increases, and as the cycle tension increase the modulus also increases, reaching a maximum of 900 PSI at 275% stretch.Funding was provided by the Gulf of Maine Ocean Observing System (GoMOOS under ONR grant N0014-01-1-0999), NOAA-UNH CINEMAR (NOAA Grant Number NA16RP1718), and GLOBEC (NSF OCE93-13670 and OCE02-27679)

Quantitative PCR Profiling of Escherichia coli in Livestock Feces Reveals Increased Population Resilience Relative to Culturable Counts under Temperature Extremes

The relationship between culturable counts (CFU) and quantitative PCR (qPCR) cell equivalent counts ofEscherichia coliin dairy feces exposed to different environmental conditions and temperature extremes was investigated. Fecal samples were collected in summer and winter from dairy cowpats held under two treatments: field-exposed versus polytunnel-protected. A significant correlation in quantifiedE. coliwas recorded between the qPCR and culture-based methods (r= 0.82). Evaluation of the persistence profiles ofE. coliover time revealed no significant difference in theE. colinumbers determined as either CFU or gene copies during the summer for the field-exposed cowpats, whereas significantly higher counts were observed by qPCR for the polytunnel-protected cowpats, which were exposed to higher ambient temperatures. In winter, the qPCR returned significantly higher counts ofE. colifor the field-exposed cowpats, thus representing a reversal of the findings from the summer sampling campaign. Results from this study suggest that with increasing time post-defecation and with the onset of challenging environmental conditions, such as extremes in temperature, culture-based counts begin to underestimate the true resilience of viableE. colipopulations in livestock feces. This is important not only in the long term as the Earth changes in response to climate-change drivers but also in the short term during spells of extremely cold or hot weather

SLC19A1 transports immunoreactive cyclic dinucleotides.

The accumulation of DNA in the cytosol serves as a key immunostimulatory signal associated with infections, cancer and genomic damage1,2. Cytosolic DNA triggers immune responses by activating the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway3. The binding of DNA to cGAS activates its enzymatic activity, leading to the synthesis of a second messenger, cyclic guanosine monophosphate-adenosine monophosphate (2'3'-cGAMP)4-7. This cyclic dinucleotide (CDN) activates STING8, which in turn activates the transcription factors interferon regulatory factor 3 (IRF3) and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB), promoting the transcription of genes encoding type I interferons and other cytokines and mediators that stimulate a broader immune response. Exogenous 2'3'-cGAMP produced by malignant cells9 and other CDNs, including those produced by bacteria10-12 and synthetic CDNs used in cancer immunotherapy13,14, must traverse the cell membrane to activate STING in target cells. How these charged CDNs pass through the lipid bilayer is unknown. Here we used a genome-wide CRISPR-interference screen to identify the reduced folate carrier SLC19A1, a folate-organic phosphate antiporter, as the major transporter of CDNs. Depleting SLC19A1 in human cells inhibits CDN uptake and functional responses, and overexpressing SLC19A1 increases both uptake and functional responses. In human cell lines and primary cells ex vivo, CDN uptake is inhibited by folates as well as two medications approved for treatment of inflammatory diseases, sulfasalazine and the antifolate methotrexate. The identification of SLC19A1 as the major transporter of CDNs into cells has implications for the immunotherapeutic treatment of cancer13, host responsiveness to CDN-producing pathogenic microorganisms11 and-potentially-for some inflammatory diseases