173 research outputs found
Experimental and Modeling Improvements to a Co-Fluid Cycle Utilizing Ionic Liquids and Carbon Dioxide
Carbon dioxide is undergoing a renaissance as an alternative to synthetic refrigerants due to its environmental advantages in addition to a high density and excellent transport properties. A weakness of carbon dioxide is having a critical point which occurs at a lower temperature and higher pressure than most other fluids used as refrigerants. This combination leads to high operating pressures, especially on the heat rejection side of the thermodynamic cycle. Ionic liquids, which are salts which remain in their liquid phase at room temperatures, have been shown to strongly absorb carbon dioxide. Due to recent advances in ionic liquids, the cation and anion groups are able to be formulated to tailor a variety of fluid properties including liquid-vapor equilibrium characteristics. By selecting appropriate ionic liquids, it is possible to reduce the operating pressure of an air-conditioning system utilizing carbon dioxide to be in the range of conventional refrigerants. Not only are ionic liquids able to physically absorb volatile refrigerants as in other co-fluid cycles, but ionic liquids also offer the possibility of chemical absorption thereby giving the opportunity for greater enthalpy changes. Conceptually, the ionic co-fluid cycle is similar to a traditional vapor compression cycle. In the high pressure heat exchanger, heat is rejected to lower the enthalpy and to absorb carbon dioxide into the ionic liquid. The enthalpy is further reduced in an internal heat exchanger before the high pressure liquid is passed through a valve to decrease the pressure which causes the fluid mixture to cool. Heat is absorbed by the mixture from the environment, thus boiling additional carbon dioxide. After passing through an internal heat exchanger, the fluid is mechanically compressed and the cycle is repeated. System modeling work was utilized to identify important thermodynamic characteristics for achieving good performance. These characteristics included heats of mixing, solubility, entropy of mixing, and viscosity. Using experimentally and numerically determined IL-CO2 mixture properties, system models were able to predicatively select anion and cation pairs for optimizing performance. The ionic liquids selected from the modeling exercises were subsequently synthesized for demonstration in a laboratory. An air conditioning system was built from components designed for use with conventional refrigerants. The system was installed in a facility which was instrumented to measure air and refrigerant pressures and temperatures. Air flow rate and temperature information allowed the cooling capacity to be measured. The power consumption of the pump and compressor used to circulate the working fluids was measured so that COP could be determined. Modeling results were validated with experimental findings. The emphasis of modeling and experiments was to determine the effect of operational parameters on system performance. The loading of ionic liquid and carbon dioxide, along with valve opening and compressor speed, was found to dramatically alter the operating pressures. The difference and ratio between high and low side pressures directly affected the specific cooling capacity and COP, respectively. While the model had strong agreement with the experimental results, non-idealities to be incorporated in more sophisticated models are identified
B-Amyloid of Alzheimer\u27s Disease Induces Reactive Gliosis that Inhibits Axonal Outgrowth
Pathological lesions in the brains of patients with Alzheimer\u27s disease (AD) are characterized by dense deposits of the protein ,B-amyloid. The link between the deposition of B-amyloid in senile plaques and AD-associated pathology is, at present, controversial since there have been conflicting reports on whether the 39- 43 amino acid B-amyloid sequence is toxic or trophic to neurons. In this report, we show that B-amyloid peptide when presented as an insoluble substrate which mimics its conformation in vivo can induce cortical glial cells in vitro and in vivo to locally deposit chondroitin sulfate containingproteoglycan. In vitro the proteoglycan-containing matrix deposited by glia on B-amyloid blocks the usual ability of the peptide to allow cortical neurons to adhere and grow. Chondroitin sulfate-containing proteoglycan was also found in senile plaques of human AD tissue. We suggest that an additional effect of B-amyloid in the brain, which compounds the direct effects of ,8- amyloid on neurons, is mediated by the stimulation of astroglia to become reactive. Once in the reactive state, glial cells deposit large amounts of growth-inhibitory molecules within the neuropil which could impair neuronal process survival and regeneration leading to neurite retraction and/or dystrophy around senile plaques in AD
Heterologous expression and characterization of CpI, OcpA, and novel serine-type carboxypeptidase OcpB from Aspergillus oryzae
In the genome of Aspergillus oryzae, 12 genes have been predicted to encode serine-type carboxypeptidases. However, the carboxypeptidase activities of the proteins encoded by these genes have not yet been confirmed experimentally. In this study, we have constructed three of these 12 genes overexpressing strains using Aspergillus nidulans and characterized their overproduced recombinant proteins. Of these three genes, one was previously named cpI; the other two have not been reported yet, and hence, we named them ocpA and ocpB. The recombinant proteins released amino acid residues from the C terminus of peptides, and the activity of the enzymes was inhibited by phenylmethylsulfonyl fluoride, indicating the enzymes to be serine-type carboxypeptidases. Recombinant OcpA, OcpB, and CpI were stable at 45Β°C, 55Β°C, and 55Β°C, respectively, at a low pH. The enzymatic properties of recombinant OcpB were different from those of any reported serine-type carboxypeptidase. On the other hand, recombinant OcpA had similar enzymatic properties to A. oryzae carboxypeptidases O1 and O2. The DNA and N-terminal amino acid sequences of carboxypeptidases O1 and O2 from A. oryzae IAM2640 were similar to those of OcpA. Result of transcriptional analysis of ocpA, ocpB, and cpI suggest differences in transcriptional regulation between these genes
Importance of Post-Translational Modifications for Functionality of a Chloroplast-Localized Carbonic Anhydrase (CAH1) in Arabidopsis thaliana
Background: The Arabidopsis CAH1 alpha-type carbonic anhydrase is one of the few plant proteins known to be targeted to the chloroplast through the secretory pathway. CAH1 is post-translationally modified at several residues by the attachment of N-glycans, resulting in a mature protein harbouring complex-type glycans. The reason of why trafficking through this non-canonical pathway is beneficial for certain chloroplast resident proteins is not yet known. Therefore, to elucidate the significance of glycosylation in trafficking and the effect of glycosylation on the stability and function of the protein, epitope-labelled wild type and mutated versions of CAH1 were expressed in plant cells. Methodology/Principal Findings: Transient expression of mutant CAH1 with disrupted glycosylation sites showed that the protein harbours four, or in certain cases five, N-glycans. While the wild type protein trafficked through the secretory pathway to the chloroplast, the non-glycosylated protein formed aggregates and associated with the ER chaperone BiP, indicating that glycosylation of CAH1 facilitates folding and ER-export. Using cysteine mutants we also assessed the role of disulphide bridge formation in the folding and stability of CAH1. We found that a disulphide bridge between cysteines at positions 27 and 191 in the mature protein was required for correct folding of the protein. Using a mass spectrometric approach we were able to measure the enzymatic activity of CAH1 protein. Under circumstances where protein N-glycosylation is blocked in vivo, the activity of CAH1 is completely inhibited. Conclusions/Significance: We show for the first time the importance of post-translational modifications such as N-glycosylation and intramolecular disulphide bridge formation in folding and trafficking of a protein from the secretory pathway to the chloroplast in higher plants. Requirements for these post-translational modifications for a fully functional native protein explain the need for an alternative route to the chloroplast.This work was supported by the Swedish Research Council (VR), the Kempe Foundations and Carl Tryggers Foundation to GS, and grant numbers BIO2006-08946 and BIO2009-11340 from the Spanish Ministerio de Ciencia e InnovaciΓ³n (MICINN) to A
A Critical Tryptophan and Ca2+ in Activation and Catalysis of TPPI, the Enzyme Deficient in Classic Late-Infantile Neuronal Ceroid Lipofuscinosis
Tripeptidyl aminopeptidase I (TPPI) is a crucial lysosomal enzyme that is deficient in the fatal neurodegenerative disorder called classic late-infantile neuronal ceroid lipofuscinosis (LINCL). It is involved in the catabolism of proteins in the lysosomes. Recent X-ray crystallographic studies have provided insights into the structural/functional aspects of TPPI catalysis, and indicated presence of an octahedrally coordinated Ca(2+).Purified precursor and mature TPPI were used to study inhibition by NBS and EDTA using biochemical and immunological approaches. Site-directed mutagenesis with confocal imaging technique identified a critical W residue in TPPI activity, and the processing of precursor into mature enzyme.NBS is a potent inhibitor of the purified TPPI. In mammalian TPPI, W542 is critical for tripeptidyl peptidase activity as well as autocatalysis. Transfection studies have indicated that mutants of the TPPI that harbor residues other than W at position 542 have delayed processing, and are retained in the ER rather than transported to lysosomes. EDTA inhibits the autocatalytic processing of the precursor TPPI.We propose that W542 and Ca(2+) are critical for maintaining the proper tertiary structure of the precursor proprotein as well as the mature TPPI. Additionally, Ca(2+) is necessary for the autocatalytic processing of the precursor protein into the mature TPPI. We have identified NBS as a potent TPPI inhibitor, which led in delineating a critical role for W542 residue. Studies with such compounds will prove valuable in identifying the critical residues in the TPPI catalysis and its structure-function analysis
The systematic significance of achene micromorphology in Carex retrorsa (Cyperaceae)
Scanning electron microscopy (SEM) was used to examine achene epidermal features of nine Carex species within sections Vesicariae and Lupulinae. Two major epidermal features are recognized in this study: the epidermal layers with one or two primary silicon bodies in each cell and the absence of any primary silicon body. Comparative data of achene morphology using SEM, and evidence compiled from previous investigations, support the placement of Carex retrorsa within section Vesicariae. Β© 1987 The New York Botanical Garden
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