Cynodon dactylon (L) Pers (Poaceae) root extract induces apoptotic cell death via the cyclin D1 pathway in human nasopharyngeal carcinoma cells in vitro and in vivo

Purpose: To investigate the activity of Cynodon dactylon root extract as a potential anticancer agent.Methods: Human nasopharyngeal carcinoma HNK-1 cells were treated with ethanol extract of C. dactylon roots. After treatment with C. dactylon root extract, cells were assessed for their cytotoxicity, metabolic status, apoptosis and cell cycle progression. The anticancer activity of the extract was also determined in vivo using a mouse model.Results: C. dactylon extract exhibited significant cytotoxicity and arrested cell cycle in HNK-1 cells in a dose-dependent manner. Fluorescence-activated cell sorting (FACS) data revealed that it also induced apoptotic cell death. Rb phosphorylation and mRNA levels of cyclin E were significantly reduced. Moreover, C. dactylon extract caused a significant reduction in tumor size and volume in vivo.Conclusion: C. dactylon root extract induces apoptosis in nasopharyngeal cancer cells via cyclin D1 pathway and inhibits tumor growth in vivo.Keywords: Cynodon dactylon, Anticancer, Cyclin D1 pathway, Apoptosis, Nasopharyngeal carcinom

The Online Data Quality Monitoring System at BESIII

The online Data Quality Monitoring (DQM) plays an important role in the data taking process of HEP experiments. BESIII DQM samples data from online data flow, reconstructs them with offline reconstruction software, and automatically analyzes the reconstructed data with user-defined algorithms. The DQM software is a scalable distributed system. The monitored results are gathered and displayed in various formats, which provides the shifter with current run information that can be used to find problems early. This paper gives an overview of DQM system at BESIII.Comment: Already submit to Chinese Physics

Grandparents Upbringing in Mainland China: The Status and Prospect of Research

This paper prospects the future study of grandparents upbringing by analyzing and summarizing briefly its nearly ten years’ situation in recent mainland China in terms of its concepts and types, causes, pros and cons as well as relevant countermeasures.Key words:Grandparents upbringing；Grandparent caregivers; Grandchildren；Pros and cons；Countermeasure

TNFα induces Ca2+ influx to accelerate extrinsic apoptosis in hepatocellular carcinoma cells

BACKGROUND: Tumor necrosis factor-α has been proven an effective anticancer agent in preclinical studies. However, the translation of TNFα from research to clinic has been blocked by significant systemic toxicity and limited efficacy at maximal tolerated dose, which need urgently to be solved. METHODS: The level of cytosolic Ca RESULTS: Here, we demonstrated that TNFα induced extracellular Ca CONCLUSIONS: Our study provides the evidence supporting a novel mechanism by which TNFα induces extracellular C