1,542 research outputs found
Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism
Background: The genes of plants can be up- or down-regulated during viral infection to influence the replication of viruses. Identification of these differentially expressed genes could shed light on the defense systems employed by plants and the mechanisms involved in the adaption of viruses to plant cells. Differential gene expression in Nicotiana benthamiana plants in response to infection with Bamboo mosaic virus (BaMV) was revealed using cDNA-amplified fragment length polymorphism (AFLP). Results: Following inoculation with BaMV, N. benthamiana displayed differential gene expression in response to the infection. Isolation, cloning, and sequencing analysis using cDNA-AFLP furnished 90 cDNA fragments with eight pairs of selective primers. Fifteen randomly selected genes were used for a combined virus-induced gene silencing (VIGS) knockdown experiment, using BaMV infection to investigate the roles played by these genes during viral infection, specifically addressing the means by which these genes influence the accumulation of BaMV protein. Nine of the 15 genes showed either a positive or a negative influence on the accumulation of BaMV protein. Six knockdown plants showed an increase in the accumulation of BaMV, suggesting that they played a role in the resistance to viral infection, while three plants showed a reduction in coat protein, indicating a positive influence on the accumulation of BaMV in plants. An interesting observation was that eight of the nine plants showing an increase in BaMV coat protein were associated with cell rescue, defense, death, aging, signal transduction, and energy production. Conclusions: This study reports an efficient and straightforward method for the identification of host genes involved in viral infection. We succeeded in establishing a cDNA-AFLP system to help track changes in gene expression patterns in N. benthamiana plants when infected with BaMV. The combination of both DNA-AFLP and VIGS methodologies made it possible to screen a large number of genes and identify those associated with infections of plant viruses. In this report, 9 of the 15 analyzed genes exhibited either a positive or a negative influence on the accumulation of BaMV in N. benthamiana plants
Cosmological equations and Thermodynamics on Apparent Horizon in Thick Braneworld
We derive the generalized Friedmann equation governing the cosmological
evolution inside the thick brane model in the presence of two curvature
correction terms: a four-dimensional scalar curvature from induced gravity on
the brane, and a five-dimensional Gauss-Bonnet curvature term. We find two
effective four-dimensional reductions of the Friedmann equation in some limits
and demonstrate that they can be rewritten as the first law of thermodynamics
on the apparent horizon of thick braneworld.Comment: 25 pages, no figure, a definition corrected, several references
added, more motivation and discussio
Hierarchical Neutrino Mass Matrices, CP violation and Leptogenesis
In this work we study examples of hierarchical neutrino mass matrices
inspired by family symmetries, compatible with experiments on neutrino
oscillations, and for which there is a connection among the low energy CP
violation phase associated to neutrino oscillations, the phases appearing in
the amplitude of neutrinoless double beta decay, and the phases relevant for
leptogenesis. In particular, we determine the predictions from a texture based
on an underlying SU(3) family symmetry together with a GUT symmetry, and a
strong hierarchy for the masses of the heavy right handed Majorana masses. We
also give some examples of inverted hierarchies of neutrino masses, which may
be motivated in the context of U(1) family symmetries.Comment: 34 pages. Replaced with published version -typos, corrections and
references adde
Thermodynamics of charged and rotating black strings
We study thermodynamics of cylindrically symmetric black holes. Uncharged as
well as charged and rotating objects have been discussed. We derive surface
gravity and hence the Hawking temperature and entropy for all these cases. We
correct some results in the literature and present new ones. It is seen that
thermodynamically these black configurations behave differently from
spherically symmetric objects
DNA single-strand break repair and spinocerebellar ataxia with axonal neuropathy-1
DNA single-strand breaks (SSBs) are the commonest DNA lesions arising spontaneously in cells, and if not repaired may block transcription or may be converted into potentially lethal/clastogenic DNA double-strand breaks (DSBs). Recently, evidence has emerged that defects in the rapid repair of SSBs preferentially impact the nervous system. In particular, spinocerebellar ataxia with axonal neuropathy (SCAN1) is a human disease that is associated with mutation of TDP1 (tyrosyl DNA phosphodiesterase 1) protein and with a defect in repairing certain types of SSBs. Although SCAN1 is a rare neurodegenerative disorder, understanding the molecular basis of this disease will lead to better understanding of neurodegenerative processes. Here we review recent progress in our understanding of TDP1, single-strand break repair (SSBR), and neurodegenerative disease
Reconstruction of Objects by Direct Demodulation
High resolution reconstruction of complicated objects from incomplete and
noisy data can be achieved by solving modulation equations iteratively under
physical constraints. This direct demodulation method is a powerful technique
for dealing with inverse problem in general case. Spectral and image
restorations and computerized tomography are only particular cases of general
demodulation. It is possible to reconstruct an object in higher dimensional
space from observations by a simple lower dimensional instrument through direct
demodulation. Our simulations show that wide field and high resolution images
of space hard X-rays and soft gamma rays can be obtained by a collimated
non-position-sensitive detector without coded aperture masks.Comment: 11 pages, 6 figure
On low temperature kinetic theory; spin diffusion, Bose Einstein condensates, anyons
The paper considers some typical problems for kinetic models evolving through
pair-collisions at temperatures not far from absolute zero, which illustrate
specific quantum behaviours. Based on these examples, a number of differences
between quantum and classical Boltzmann theory is then discussed in more
general terms.Comment: 25 pages, minor updates of previous versio
Kinetic energy driven superconductivity in doped cuprates
Within the t-J model, the mechanism of superconductivity in doped cuprates is
studied based on the partial charge-spin separation fermion-spin theory. It is
shown that dressed holons interact occurring directly through the kinetic
energy by exchanging dressed spinon excitations, leading to a net attractive
force between dressed holons, then the electron Cooper pairs originating from
the dressed holon pairing state are due to the charge-spin recombination, and
their condensation reveals the superconducting ground-state. The electron
superconducting transition temperature is determined by the dressed holon pair
transition temperature, and is proportional to the concentration of doped holes
in the underdoped regime. With the common form of the electron Cooper pair, we
also show that there is a coexistence of the electron Cooper pair and
antiferromagnetic short-range correlation, and hence the antiferromagnetic
short-range fluctuation can persist into the superconducting state. Our results
are qualitatively consistent with experiments.Comment: 6 pages, Revtex, two figures are included, corrected typo
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