Response of oxidative stress and isoflavone treatment on superoxide dismutase enzyme activities and lipid peroxidation in rat’s liver

Oxidative stress is defined as over-production of free radicals which lead to cells damage, pathological condition and cell death. The objective of this study was to analyze respond of oxidative stress and isoflavone treatment on superoxide dismutase (SOD) enzyme activities and lipid peroxidation in rat liver. A total of fifteen male Spraque Dawley rats were used in this study. They were sub-divided into three groups; (1) a negative control group, (2) a stress oxidative group, and (3) treatment by stress condition followed by treatment with isoflavone. Stress condition was achieved by five days fasting together with swimming for 5 mins/day and only drinking water ad libitum. Isoflavone was orally administrated on a dose of 1 mg/200g bw/day for five days. At the end of the experiment, rats were sacrificed by anesthesia. Liver was collected for analysis of SOD enzyme activities, SOD immunohistochemical analysis, and malondialdehyde (MDA) level. Result showed that stress condition increase free radicals that showed by decreased SOD activity, and increased MDA level. Isoflavone treatment could get over reduction of SOD and prevented increase of MDA level in the liver of rats under stress conditions. Key Words: Oxidative Stress, Isoflavone, Rat, Superoxide Dismutase, Malondialdehyd

Sifat Fisik Daging Domba Yang Diberi Perlakuan Stimulasi Listrik Voltase Rendah Dan Injeksi Kalsium Klorida

Effect of low voltage electrical stimulation (LVES; 45 volt, 3 amps for approx 100 sec) and calcium chloride (CaCl2) injection on physical properties of meat were evaluated. Six mutton were devided into 3 groups. One carcass of each group was subjected to within 30 min postmortem (PM). After LVES, longissimi thoracis et lumbarum muscles were removed and treated: without CaCl2 injection, CaCl2 injection (200 mM, 5% w/w) at about 2 h and 24 h PM. Samples were stored in vacuum pack at 1 + 10 C. Physical properties evaluated were Warner-Bratzler (WB) shear force, cooking loss and sarcomere length. The LVES had no significant effect on WB shear force, cooking loss and sarcomere length. There wasn't interaction effect between LVES and CaCl2 injection on WB shear force, cooking loss and sarcomere length. CaCl2 injection decreased sarcomere length (

Profile of blood glucose and ultrastucture of beta cells pancreatic islet in alloxan compound induced rats

Diabetes is marked by elevated levels of blood glucose, and progressive changes of the structure of pancreatic islet histopathology. The objective of this research was to analyse the glucose level and histophatological feature in pancreatic islet in alloxan compound induced rats. A total of ten male Spraque Dawley rats of 2 months old were used in this study. The rats were divided into two groups: (1) negative control group (K-), and (2) positif induced alloxan group (diabetic group =DM). The rats were induced by a single dose intraperitonial injection of alloxan compound 120 mg/kg of body weight. The treatment was conducted for 28 days. Blood glucose levels of rats were analysed at 0, 4, 7, 14, 21, and 28 days following treatment. At the end of the experiment, rats were sacrificed by cervical dislocation. Pancreas was collected for analysis of histopathological study by Immunohistochemical technique, and ultrastructural study using transmission electron microscope (TEM). The result showed that Langerhans islet of diabetic rat (rat of DM group) showed a marked reduction of size, number of Langerhans islet of diabetic rat decrease, and characterized by hyperglycemic condition. By using TEM, beta cells of DM group showed the rupture of mitochondrial membrane, the lost of cisternal structure of inner membrane of mitocondria, reduction of insulin secretory granules, linkage between cells acinar with free Langerhans islet, and the caryopicnotic of nucleus. Key words: Alloxan, Beta Cells, Rat, Blood Glucose, Immunohistochemistr

Sifat Fisik Daging Domba yang Diberi Perlakuan Stimulasi Listrik Voltase Rendah dan Injeksi Kalsium Klorida

Effect of low voltage electrical stimulation (LVES; 45 volt, 3 amps for approx 100 sec) and calcium chloride (CaCl2) injection on physical properties of meat were evaluated. Six mutton were devided into 3 groups. One carcass of each group was subjected to within 30 min postmortem (PM). After LVES, longissimi thoracis et lumbarum muscles were removed and treated: without CaCl2 injection, CaCl2 injection (200 mM, 5% w/w) at about 2 h and 24 h PM. Samples were stored in vacuum pack at 1 + 10 C. Physical properties evaluated were Warner-Bratzler (WB) shear force, cooking loss and sarcomere length. The LVES had no significant effect on WB shear force, cooking loss and sarcomere length. There wasn't interaction effect between LVES and CaCl2 injection on WB shear force, cooking loss and sarcomere length. CaCl2 injection decreased sarcomere length (

Karakteristik Organoleptik Daging Domba yang Diberi Stimulasi Listrik Voltase Rendah dan Injeksi Kalsium Klorida

Effect of low voltage electrical stimulation (LVES; 45 volt, 3 amps for approx 100 sec) and CaCl2 injection on sensory characteristic of mutton were evaluated. Six mutton were divided into 3 groups slaughtered. One carcass of each group was subjected to LVES treatment within 30 min postmortem (PM). After LVES, longissimi thoracis et lumborum muscles were removed and treated: without CaCl2 injection, CaCl2 injection (200 mM, 5% w/w) 2 h and 24 h PM. Samples were stored in a vacuum pack at 1 ± 10C. Results showed that LVES could be used to improve colour of meat. CaCl2 injection, especially without LVES produced smooth mutton texture. CaCl2 injection 24 hr PM without LVES decreased meat elasticity. LVES with CaCl2 injection 2 hr PM was the best treatment to produce fresh mutton with good sensory characteristic. Key words : mutton, electrical stimulation, CaCl2, sensory characteristi

Production of Synbiotic Yogurt-Like Using Indigenous Lactic Acid Bacteria as Functional Food

Yoghurt is a product of fermented milk using Lactobacillus bulgaricus and Streptococcus thermophilus as culture starter. Indigenous probiotic lactic acid bacteria, Lactobacillus plantarum 2C12 or Lactobacillus acidophilus 2B4, were applied in the making of functional synbiotic yoghurt-like with 5% of fructo-oligosaccharide (FOS) as a prebiotic source. The aim of this study was to determine the best formula of functional synbiotic yoghurt-like among four formulas: F1 (L. bulgaricus + S. thermophilus), F2 (L. bulgaricus + S. thermophilus + L. plantarum 2C12), F3 (L. bulgaricus+ S. thermophilus + L. acidophilus 2B4), and F4 (L. bulgaricus + S. thermophilus + L. plantarum 2C12 + L. acidophilus 2B4) to be choosen and followed detection of it's flavor to improve the product quality and consumer acceptance. The results showed that the F3 synbiotic yogurt made from mixed culture L. bulgaricus, S. thermophilus, and L. acidophilus 2B4 had the highest antibacterial effect against Enteropathogenic Escherichia coli (EPEC). Addition of 1.75% natural corn starch as a stabilizer produced optimum improvement in yoghurt consistency and minimize whey separation. Result of sensory evaluation indicated that the yoghurt with addition of 1% strawberry flavor and 0.1% vanilla flavor were ranked at first and second. Yoghurts were still good to be consumed after 15 d storage period at the refrigeration temperature (10 oC)

Kaji Banding Morfometri Spermatozoa Sapi Bali (Bos sondaicus) Menggunaican Pewarnaan Williams, Eosin, Eosin Nigrosin dan Formol-Saline= Comparative Study of Bali Bull Cattle (Bos sondaicus) Sperm Morphometry...

Tujuan dari penelitian ini adalah untuk mengkaji morfometri spermatozoa sapi bali dengan pewarnaan Williams (W), eosin (E), eosin nigrosin (EN) dan fiksasiformol-saline (FS) sebagai data dasar yang sampai scat ini belum dilaporkan. Semen dikoleksi dengan teknik vagina buatan dari sepuluh ekor sapi di Unit Pelaksana Teknis Daerah (UPTD) Baturiti, Bali. Semen yang diperoleh dievaluasi secara makroskopis dan mikroskopis. Morfometri spermatozoa dilakukan dengan menggunakan mikrometer dengan bagian yang diukur adalah panjang dan lebar kepalapanjang ekor bagian tengah dan utamaserta panjang total sperma pada 50 sel untuk setiap sampel sebanyak 3 ulangan. Morfometri spermatozoa pada bagian panjang kepala dengan pewarnaan W (10,0510,05 pm) dan FS (10,0810,04 Rm) nyata lebih panjang (P Key Word:Morphometry,Spermatoza,Bali bull-cattl