Comparison of Organic and Integrated Nutrient Management Strategies for Reducing Soil N\u3csub\u3e2\u3c/sub\u3eO Emissions

To prevent nutrient limitations to crop growth, nitrogen is often applied in agricultural systems in the form of organic inputs (e.g., crop residues, manure, compost, etc.) or inorganic fertilizer. Inorganic nitrogen fertilizer has large environmental and economic costs, particularly for low-input smallholder farming systems. The concept of combining organic, inorganic, and biological nutrient sources through Integrated Nutrient Management (INM) is increasingly promoted as a means of improving nutrient use efficiency by matching soil nutrient availability with crop demand. While the majority of previous research on INM has focused on soil quality and yield, potential climate change impacts have rarely been assessed. In particular, it remains unclear whether INM increases or decreases soil nitrous oxide (N2O) emissions compared to organic nitrogen inputs, which may represent an overlooked environmental tradeoff. The objectives of this review were to (i) summarize the mechanisms influencing N2O emissions in response to organic and inorganic nitrogen (N) fertilizer sources, (ii) synthesize findings from the limited number of field experiments that have directly compared N2O emissions for organic N inputs vs. INM treatments, (iii) develop a hypothesis for conditions under which INM reduces N2O emissions and (iv) identify key knowledge gaps to address in future research. In general, INM treatments having low carbon to nitrogen ratio C:N (2O emissions

Knowledge of Pollinator Conservation and Associated Plant Recommendations in the Horticultural Retail Industry

Pollinating insects are integral to the health of all terrestrial ecosystems and agriculture worldwide. Urbanization can greatly reduce nutritional resources and habitat for pollinators. However, these losses can be mitigated through targeted landscape practices, such as planting nectar- and pollen-rich plants and managing pollinator habitat in urban areas, especially home landscapes. As homeowners attempt to conserve pollinators through horticultural practices, they often seek the advice and guidance of horticulture retail employees. The knowledge horticulture employees have about pollinators and the recommendations they provide to customers is largely unknown. A nationwide survey was developed and distributed with the objectives to 1) assess employee knowledge about pollinators and pollination biology, 2) discover what plant and management recommendations employees were giving customers pertaining to pollinator conservation, and 3) determine where to focus possible education and outreach, as well as which topics to focus educational programs on. Our findings suggest, among our respondents, that overall knowledge was adequate, with a mean score (±SD) being 8.37 (±3.23) of a possible range of 0–14 points. Uncertified and part-time employees were identified as having significantly lower scores. The subject of plant selection was found to have the largest gap in knowledge, with a mean score of 1.82 (±0.62) of a possible three points. We identified several opportunities for educational outreach, aimed at improving employee and customer knowledge on this important subject

Air-propelled Abrasive Grit can Damage the Perennial Weed Quackgrass

our field experiments were conducted during 2016 and 2017 to evaluate the efficacy of preemergence herbicides to control glyphosate-resistant common ragweed in corn. Dicamba, dicamba/atrazine, mesotrione + atrazine, isoxaflutole + atrazine, saflufenacil/dimethenamid-P, and S-metolachlor/mesotrione/bicyclopyrone/atrazine were the most efficacious herbicides, providing 94%–100% control of GR common ragweed and reducing density and biomass 98%–100%

Post-termination Effects of Cover Crop Monocultures and Mixtures on Soil Inorganic Nitrogen and Microbial Communities on Two Organic Farms in Illinois

Cover crops can continue to affect agricultural systems even after they have been terminated by influencing nitrogen dynamics and by altering soil microbial communities. These post-termination effects can influence soil fertility, weed pressure, and the dynamics of potential plant pathogens in the narrow window of time between cover crop termination and cash crop emergence. We evaluated the post-termination effects of 12 different spring-sown cover crop mixtures and monocultures on soil nitrogen and microbial communities on two different organic farms in Central Illinois (on Lawson silt loam soil) and Northern Illinois (on Virgil silt loam soil). In comparison to control plots with no cover crops, all cover crop treatments significantly reduced soil nitrate levels but increased the potentially mineralizable nitrogen pool following termination. Nitrate levels of cover crop plots approached those of controls after 2 and 4 weeks, respectively, but potentially mineralizable nitrogen levels in cover plots remained elevated for at least 4 weeks following termination. Monocultures of Brassica cover crops showed the greatest decrease in soil nitrate, while Brassicas and unplanted control plots containing high biomass of weeds showed the greatest increase in potentially mineralizable nitrogen in comparison to plant-free control plots. In contrast to their effect on soil nitrogen, cover crops had very limited impact on the composition of soil microbial communities. Overall microbial community composition varied across sites and years, and only soil fungi significantly responded to cover cropping treatments. Nevertheless, we found that some highly correlated groups of soil microbes showed significant responses to soil nitrate and to high plant biomass. Key members of these correlated groups included ammonia-oxidizing organisms and saprotrophic fungi. Our results suggest that cover crops may reduce the potential for springtime nitrogen leaching losses by retaining nitrogen in the soil organic pool, and they may also have impacts on the soil microbial community that are particularly relevant for nitrogen cycling and decomposition of plant residues

Evaluating the Impact of Grafting on Local Tomato Production in Nebraska

Grafting has been successfully used in vegetable production for tomatoes, peppers, eggplants, cucumbers, and watermelon. Besides its usefulness for managing soil-borne diseases, grafting can improve nutrient uptake and yield. However, few studies have assessed the effects of grafting and soil fertility management on yield of open field-grown tomatoes in the Midwest. Therefore, the objective of this two-year research was to better document the effects of grafting heirloom and hybrid tomato cultivar onto hybrid tomato rootstocks on tomato yield and quality. The field experiments were located at the University of Nebraska Lincoln - East Campus in Lincoln, West Central Research and Extension Center in North Platte, and a farm trial at Perkarek’s Vegetable Farm near Dwight, Nebraska. Two determinant fresh market tomatoes, ‘Nebraska Wedding’ and ‘BHN-589’, were grafted onto two rootstocks, ‘Estamino’ and ‘Maxifort,’ with the non-grafted determinant market tomatoes as a control. During the second year of this study, fertilizer treatment was introduced with two N rates (0% and 100%). At the end of the growing season, ripe tomatoes were harvested on a weekly basis, and yield was determined by weighing all tomatoes from the five plants in each experimental unit. Overall, there was no consistent improvement in total yield for any of the grafting treatments, and the estimated total mean yield of BHN-589 was at least 50% more than Nebraska Wedding. Moreover, there was no interaction effect between grafting and fertilizing treatment within each location. Results from this study suggest the need for more assessment on the effect of tomato grafting under different environmental conditions

Increased weed diversity, density and above-ground biomass in long-term organic crop rotations

While weed management is consistently a top priority among farmers, there is also growing concern for the conservation of biodiversity. Maintaining diverse weed communities below bioeconomic thresholds may provide ecosystem services for the crop and the surrounding ecosystem. This study was conducted to determine if weed diversity, density and biomass differ within and among organic and conventional crop rotations. In 2007 and 2008, we sampled weed communities in four long-term crop rotations near Mead, Nebraska using seedbank analyses (elutriation and greenhouse emergence) and above-ground biomass sampling. Two conventional crop rotations consisted of a corn (Zea mays) or sorghum (Sorghum bicolor)–soybean (Glycine max)–sorghum or corn–soybean sequence and a diversified corn or sorghum–sorghum or corn– soybean–wheat (Triticum aestivum) sequence. Two organic rotations consisted of an animal manure-based soybean–corn or sorghum–soybean–wheat sequence and a green manure-based alfalfa (Medicago sativa)–alfalfa–corn or sorghum–wheat sequence. Species diversity of the weed seedbank and the above-ground weed community, as determined by the Shannon diversity index, were greatest in the organic green manure rotation. Averaged across all sampling methods and years, the weed diversity index of the organic green manure rotation was 1.07, followed by the organic animal manure (0.78), diversified conventional (0.76) and conventional (0.66) rotations. The broadleaf weed seedbank density in the tillage layer of the organic animal manure rotation was 1.4x, 3.1x and 5.1x greater than the organic green manure, diversified conventional and conventional rotations, respectively. The grass weed seedbank density in the tillage layer of the organic green manure rotation was 2.0x, 6.1x and 6.4x greater than the organic animal manure, diversified conventional and conventional rotations, respectively. The above-ground weed biomass was generally greatest in the organic rotations. The broadleaf weed biomass in sorghum and wheat did not differ between organic and conventional rotations (CRs), but grass weed biomass was greater in organic compared to CRs for all crops. The above-ground weed biomass did not differ within CRs, and within organic rotations the grass weed biomass was generally greatest in the organic green manure rotation. The weed seedbank and above-ground weed communities that have accumulated in these rotations throughout the experiment suggest a need for greater management in long-term organic rotations that primarily include annual crops. However, results suggest that including a perennial forage crop in organic rotations may reduce broadleaf weed seedbank populations and increase weed diversity

Biobased Sprayable Mulch Films Suppressed Annual Weeds in Vegetable Crops

Biobased sprayable mulch (BSM) films are a potential alternative to herbicides, polyethylene plastic mulch film, and hand weeding for specialty crops. We developed a series of BSM films using locally available biomaterials [including corn (Zea mays) starch, glycerol, keratin hydrolysate, corn gluten meal, corn zein, eggshells, and isolated soy (Glycine max) protein] and tested their effects on weeds and crop yield during a total of seven greenhouse or field trials between 2017 and 2019 in Nebraska, USA. Application rates of BSM films applied in pots (greenhouse), planting holes in plastic film (field), or bed tops (field) ranged from 0.9 to 18.2 L•m-2 ; they were applied before and after the emergence of weeds. Weed control efficacy was variable, and results of greenhouse pots were rarely replicated under field conditions. Increasing the viscosity of the final suspension tested [BSM7; a mix of corn starch (72.8 g•L-1 ), glycerol (184.7 mL•L-1 ), keratin hydrolysate (733.3 mL•L-1 ), corn zein (19.8 g•L-1 ), and isolated soy protein (19.8 g•L-1 )] reduced weed biomass by more than 96% in field-grown kale (Brassica oleracea var. sabellica) when applied to bare soil bed tops before or after weed emergence, but kale yield in treated plots was not different from the weedy control. The results demonstrated the potential for postemergence applications of BSM films, which increase application timing flexibility for growers. Further research is needed to explore the effects of BSM films on soil properties and crop physiology and yield

Draft Genome of the Filarial Nematode Parasite \u3ci\u3eBrugia malayi\u3c/i\u3e

Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the ∼90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict ∼11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during ∼350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design

Reconstructing and Reprogramming the Tumor-Propagating Potential of Glioblastoma Stem-like Cells

Developmental fate decisions are dictated by master transcription factors (TFs) that interact with cis-regulatory elements to direct transcriptional programs. Certain malignant tumors may also depend on cellular hierarchies reminiscent of normal development but superimposed on underlying genetic aberrations. In glioblastoma (GBM), a subset of stem-like tumor-propagating cells (TPCs) appears to drive tumor progression and underlie therapeutic resistance, yet remain poorly understood. Here, we identify a core set of neurodevelopmental TFs (POU3F2, SOX2, SALL2, OLIG2) essential for GBM propagation. These TFs coordinately bind and activate TPC-specific regulatory elements, and are sufficient to fully reprogram differentiated GBM cells to ‘induced’ TPCs, recapitulating the epigenetic landscape and phenotype of native TPCs. We reconstruct a network model that highlights critical interactions and identifies novel therapeutic targets for eliminating TPCs. Our study establishes the epigenetic basis of a developmental hierarchy in GBM, provides detailed insight into underlying gene regulatory programs, and suggests attendant therapeutic strategies

Diversification of Organic Cropping Systems with Cover Crop Mixtures: Influence on Weed Communities, Soil Microbial Community Structure, Soil Moisture and Nitrogen, and Crop Yield

Organic grain cropping systems typically depend on intensive mechanical cultivation for weed control and manure or compost applications to meet plant nutrient demands. However, cover crops may contribute to weed suppression and soil fertility, potentially increasing crop yield and sustainability of the system. The utility of individual cover crop species have been well documented, but the agronomic benefits of diverse cover crop mixtures have received less attention. Cover crop mixtures are an appealing option for farmers, as increasing species diversity has been shown to increase resource-use efficiency, stability, resiliency, and productivity of plant communities. Despite the growing interest in cover crop mixtures, little is known about the effect of increasing cover crop diversity on cropping system performance. Moreover, organic farmers have questions about the most effective method for cover crop mixture termination. In an effort to increase knowledge about cover crop mixtures and management for the western Corn Belt, an organic cropping systems trial was initiated in 2009 at the UNL ARDC near Mead, NE. Spring-sown mixtures of cover crops, ranging from two to eight species, were included in a sunflower – soybean – corn crop rotation. Cover crops were planted in late-March and terminated mechanically with either a field disk or sweep plow undercutter in late-May. Changes in cover crop mixture influenced cover crop productivity and early-season weed biomass, while termination method drove differences in weed community composition, soil microbial community structure, soil moisture and nitrogen, and crop yield. Interestingly, the management of ambient weed communities as a cover crop led to unique shifts in soil microbial community structure, but did not alter soil nitrogen or crop yield when compared to cover crop mixtures. When considering cropping system performance in combination with potential environmental benefits, diverse cover crop mixtures paired with a sweep plow undercutter for termination seems to be a profitable and sustainable management option for organic grain farmers in the western Corn Belt