COMBINED USE OF OPEN-AIR AND INDOOR FUMIGATION SYSTEMS TO STUDY EFFECTS OF SO-2 ON LEACHING PROCESSES IN SCOTS PINE LITTER

Both an open-air fumigation system and a laboratory-based system were used to expose decomposing Scots pine (Pinus sylvestris L.) needles to controlled concentrations of SO2 (arithmetric mean less-than-or-equal-to 48 nl litre-1) during a period, in total, of 301 days. The experimental design involved reciprocal litter transplants from 'clean' to 'polluted' air and vice versa, using the two fumigation systems. The objectives were (1) to observe the effects of SO2 on leachate and litter chemistry, (2) to assess whether pollution-induced changes are reversible in clean air, and (3) to test the suitability of small-scale fumigation chambers (litter microcosms) compared with open-air systems in soil studies.Through the formation of SO4(2-) ions, dry-deposited SO2 exhibited a marked capacity to remove 'base' cations (Ca2+, Mg2+ and K+) from decomposing pine needles, and also to acidify litter leachates (as indicated by proton fluxes from the litter). When litter was transferred from polluted air (48 nl litre-1 SO2, in the open-air system) to either clean or polluted air in the laboratory, the effects of prior exposure to SO2 on leachate composition were still evident even after 86 days: the role of base cation depletion within the litter, caused by SO42- -induced leaching, is discussed.Data for SO42- fluxes in leachates collected from the small-scale chambers indicated that dry deposition velocities for SO2 were not anomalously high within this fumigation system. It is therefore concluded that microcosm studies can provide information complementary to the open-air fumigation approach in soils research.</p

Amylin in the periphery II: An updated mini-review

Amylin is a polypeptide that is cosecreted with insulin from the beta cells of the pancreas. Therefore, in states of diabetes in which the beta-cell mass is largely depleted or dysfunctional, insulin and amylin secretion are also lost or dysregulated. While the soluble monomeric form of amylin acts as a hormone that alters physiological responses related to feeding and acts as a specific growth factor, there has been renewed interest in the less-soluble oligomeric and insoluble polymeric forms of human (also monkey and cat) amylin that may contribute to the establishment of a pathophysiological pathway to overt diabetes. With this discovery has grown the hope of minimizing, with appropriate therapy, these toxic forms to preserve the functional (c) not-cell mass. Human beta cells may also be more vulnerable to these forms and one risk factor, a higher fat diet, may promote toxic forms. The generation and utilities of transgenic rodent models, which express enhanced levels of human amylin, have been accompanied by strategies that may lead to the reduction of toxic forms and associated risk factors. The successful definition and faithful expression of the physiological receptors (and complexes) for amylin that may differ for each target organ is an important development in the field of amylin research generally. Besides the heuristic value for the understanding of the molecular biology of receptors, the opportunity to screen and identify nonpeptide analogues that bind the physiological receptors has important implications for biomedicine and clinical practice in relation to treatments for diabetic complications, bone diseases, and eating disorders. In particular, in their capacities to mimic the effects of amylin as a growth factor, amylin analogues may prove useful in the stimulation of beta-cell mass (in conjunction with other factors), reduce the activity of the osteoclast population, and stimulate the regeneration of proximal tubules following toxic insult (and thus avoid the development of renal insufficiency)

Peripheral Calcitonin Gene-Related Peptide Receptor Activation and Mechanical Sensitization of the Joint in Rat Models of Osteoarthritis Pain

OBJECTIVE: To investigate the role of the sensory neuropeptide calcitonin gene-related peptide (CGRP) in peripheral sensitization in experimental models of osteoarthritis (OA) pain. METHODS: Experimental knee OA was induced in rats by intraarticular injection of monosodium iodoacetate (MIA) or by transection of the medial meniscus (MMT). Single-unit recordings of joint-innervating nociceptors were obtained in MIA- and saline-treated rats following administration of CGRP or the CGRP receptor antagonist CGRP 8-37. Effects of CGRP 8-37 were also examined in rats that underwent MMT and sham operations. Protein and messenger RNA (mRNA) levels of CGRP receptor components in the L3-L4 dorsal root ganglion (DRG) were investigated following MIA treatment. RESULTS: In both the MIA and MMT groups, the mechanical sensitivity of joint nociceptors was enhanced compared to that in the control groups. Exogenous CGRP increased mechanical sensitivity in a greater proportion of joint nociceptors in the MIA-treated rats than in the saline-treated rats. Local blockade of endogenous CGRP by CGRP 8-37 reversed both the MIA- and MMT-induced enhancement of joint nociceptor responses. Joint afferent cell bodies coexpressed the receptor for CGRP, called the calcitonin-like receptor (CLR), and the intracellular accessory CGRP receptor component protein. MIA treatment increased the levels of mRNA for CLR in the L3-L4 DRG and the levels of CLR protein in medium and large joint afferent neurons. CONCLUSION: Our findings provide new and compelling evidence implicating a role of CGRP in peripheral sensitization in experimental OA. Our novel finding of CGRP-mediated control of joint nociceptor mechanosensitivity suggests that the CGRP receptor system may be an important target for the modulation of pain during OA. CGRP receptor antagonists recently developed for migraine pain should be investigated for their efficacy against pain in OA

Effects of Warming on Shrub Abundance and Chemistry Drive Ecosystem-Level Changes in a Forest-Tundra Ecotone

Tundra vegetation is responding rapidly to on-going climate warming. The changes in plant abundance and chemistry might have cascading effects on tundra food webs, but an integrated understanding of how the responses vary between habitats and across environmental gradients is lacking. We assessed responses in plant abundance and plant chemistry to warmer climate, both at species and community levels, in two different habitats. We used a long-term and multisite warming (OTC) experiment in the Scandinavian forest–tundra ecotone to investigate (i) changes in plant community composition and (ii) responses in foliar nitrogen, phosphorus, and carbon-based secondary compound concentrations in two dominant evergreen dwarf-shrubs (Empetrum hermaphroditum and Vaccinium vitis-idaea) and two deciduous shrubs (Vaccinium myrtillus and Betula nana). We found that initial plant community composition, and the functional traits of these plants, will determine the responsiveness of the community composition, and thus community traits, to experimental warming. Although changes in plant chemistry within species were minor, alterations in plant community composition drive changes in community-level nutrient concentrations. In view of projected climate change, our results suggest that plant abundance will increase in the future, but nutrient concentrations in the tundra field layer vegetation will decrease. These effects are large enough to have knock-on consequences for major ecosystem processes like herbivory and nutrient cycling. The reduced food quality could lead to weaker trophic cascades and weaker top down control of plant community biomass and composition in the future. However, the opposite effects in forest indicate that these changes might be obscured by advancing treeline forests

Local magnitude discrepancies for near‐event receivers: implications for the U.K. Traffic‐Light Scheme

Local seismic magnitudes provide a practical and efficient scale for the implementation of regulation designed to manage the risk of induced seismicity, such as Traffic‐Light Schemes (TLS). We demonstrate that significant magnitude discrepancies (up to a unit higher) occur between seismic events recorded on nearby stations (<5  km) compared with those at greater distances. This is due to the influence of sedimentary layers, which are generally lower in velocity and more attenuating than the underlying crystalline basement rocks, and requires a change in the attenuation term of the ML scale. This has a significant impact on the United Kingdom’s (U.K.) hydraulic fracturing TLS, whose red light is set at ML 0.5. Because the nominal detectability of the U.K. network is ML 2, this scheme will require the deployment of monitoring stations in close proximity to well sites. Using data collected from mining events near New Ollerton, Nottinghamshire, we illustrate the effects that proximity has on travel path velocities and attenuation, then perform a damped least‐squares inversion to determine appropriate constants within the ML scale. We show that the attenuation term needs to increase from 0.00183 to 0.0514 and demonstrate that this higher value is representative of a ray path within a slower more attenuating sedimentary layer compared with the continental crust. We therefore recommend that the magnitude scale ML=log(A)+1.17log(r)+0.0514r−3.0 should be used when local monitoring networks are within 5 km of the event epicenters