TERT Promotes Epithelial Proliferation through Transcriptional Control of a Myc- and Wnt-Related Developmental Program

Telomerase serves a critical role in stem cell function and tissue homeostasis. This role depends on its ability to synthesize telomere repeats in a manner dependent on the reverse transcriptase (RT) function of its protein component telomerase RT (TERT), as well as on a novel pathway whose mechanism is poorly understood. Here, we use a TERT mutant lacking RT function (TERTci) to study the mechanism of TERT action in mammalian skin, an ideal tissue for studying progenitor cell biology. We show that TERTci retains the full activities of wild-type TERT in enhancing keratinocyte proliferation in skin and in activating resting hair follicle stem cells, which triggers initiation of a new hair follicle growth phase and promotes hair synthesis. To understand the nature of this RT-independent function for TERT, we studied the genome-wide transcriptional response to acute changes in TERT levels in mouse skin. We find that TERT facilitates activation of progenitor cells in the skin and hair follicle by triggering a rapid change in gene expression that significantly overlaps the program controlling natural hair follicle cycling in wild-type mice. Statistical comparisons to other microarray gene sets using pattern-matching algorithms revealed that the TERT transcriptional response strongly resembles those mediated by Myc and Wnt, two proteins intimately associated with stem cell function and cancer. These data show that TERT controls tissue progenitor cells via transcriptional regulation of a developmental program converging on the Myc and Wnt pathways

First Results from COPSS: The CO Power Spectrum Survey

We present constraints on the abundance of carbon monoxide in the early universe from the CO Power Spectrum Survey. We utilize a data set collected between 2005 and 2008 using the Sunyaev–Zel'dovich Array (SZA), which was previously used to measure arcminute-scale fluctuations of the cosmic microwave background. This data set features observations of 44 fields, covering an effective area of 1.7 square degrees, over a frequency range of 27–35 GHz. Using the technique of intensity mapping, we are able to probe the CO(1–0) transition, with sensitivity to spatial modes between k = 0.5–2 h Mpc^(−1) over a range in redshift of z = 2.3–3.3, spanning a comoving volume of 3.6 × 10^6 h^(−3) Mpc^3. We demonstrate our ability to mitigate foregrounds, and present estimates of the impact of continuum sources on our measurement. We constrain the CO power spectrum to P_(CO) < 2.6 × 10^4 μK^2 (h^(−1) Mpc)^3, or Δ^2_(CO)(k = 1 h Mpc^(−1)) < 1.3 × 10^3 μK^2, at 95% confidence. This limit resides near optimistic predictions for the CO power spectrum. Under the assumption that CO emission is proportional to halo mass during bursts of active star formation, this corresponds to a limit on the ratio of CO(1–0) luminosity to host halo mass of A_(CO) < 1.2 × 10^(−5) L⊙_ M_⊙^(−1). Further assuming a Milky Way-like conversion factor between CO luminosity and molecular gas mass (α_(CO) = 4.3 M_⊙ (K km s^(−1) pc^(−2))^(−1)), we constrain the global density of molecular gas to ρ_(z~3) (M_H_2) ⩽ 2.8 x 10^8 M_☉ Mpc^(-3)

Software security requirements management as an emerging cloud computing service

© 2016 Elsevier Ltd. All rights reserved.Emerging cloud applications are growing rapidly and the need for identifying and managing service requirements is also highly important and critical at present. Software Engineering and Information Systems has established techniques, methods and technology over two decades to help achieve cloud service requirements, design, development, and testing. However, due to the lack of understanding of software security vulnerabilities that should have been identified and managed during the requirements engineering phase, we have not been so successful in applying software engineering, information management, and requirements management principles that have been established for the past at least 25 years, when developing secure software systems. Therefore, software security cannot just be added after a system has been built and delivered to customers as seen in today's software applications. This paper provides concise methods, techniques, and best practice requirements engineering and management as an emerging cloud service (SSREMaaES) and also provides guidelines on software security as a service. This paper also discusses an Integrated-Secure SDLC model (IS-SDLC), which will benefit practitioners, researchers, learners, and educators. This paper illustrates our approach for a large cloud system Amazon EC2 service

Two-pion correlations in Au+Au collisions at 10.8 GeV/c per nucleon

Two-particle correlation functions for positive and negative pions have been measured in Au+Au collisions at 10.8~GeV/c per nucleon. The data were analyzed using one- and three-dimensional correlation functions. From the results of the three-dimensional fit the phase space density of pions was calculated. It is consistent with local thermal equilibrium.Comment: 5 pages RevTeX (including 3 Figures

Directed flow of antiprotons in Au+Au collisions at AGS

Directed flow of antiprotons is studied in Au+Au collisions at a beam momentum of 11.5A GeV/c. It is shown that antiproton directed flow is anti-correlated to proton flow. The measured transverse momentum dependence of the antiproton flow is compared with predictions of the RQMD event generator.Comment: 16 pages, 6 figure

Hadron yields and spectra in Au+Au collisions at the AGS

Inclusive double differential multiplicities and rapidity density distributions of hadrons are presented for 10.8 A GeV/c Au+Au collisions as measured at the AGS by the E877 collaboration. The results indicate that large amounts of stopping and collective transverse flow effects are present. The data are also compared to the results from the lighter Si+Al system.Comment: 12 pages, latex, 10 figures, submitted to Nuclear Physics A (Quark Matter 1996 Proceedings

Proton and Pion Production in Au+Au Collisions at 10.8A GeV/c

We present proton and pion tranverse momentum spectra and rapidity distributions for Au+Au collisions at 10.8A GeV/c. The proton spectra exhibit collective transverse flow effects. Evidence of the influence of the Coulomb interaction from the fireball is found in the pion transverse momentum spectra. The data are compared with the predictions of the RQMD event generator.Comment: plain tex (revtex), 24 pages Submitted to Phys. Rev.

Proton and Pion Production Relative to the Reaction Plane in Au + Au Collisions at AGS Energies

Results are presented of an analysis of proton and charged pion azimuthal distributions measured with respect to the reaction plane in Au + Au collisions at a beam momentum of about 11 AGeV/c. The azimuthal anisotropy is studied as a function of particle rapidity and transverse momentum for different centralities of the collisions. The triple differential (in rapidity, transverse momentum, and azimuthal angle) distributions are reconstructed. A comparison of the results with a previous analysis of charged particle and transverse energy flow as well as with model predictions is presented.Comment: 23 pages (LaTeX), 12 figure

Two-Proton Correlations from 14.6A GeV/c Si+Pb and 11.5A GeV/c Au+Au Central Collisions

Two-proton correlation functions have been measured in Si+Pb collisions at 14.6A GeV/c and Au+Au collisions at 11.5A GeV/c by the E814/E877 collaboration. Data are compared with predictions of the transport model RQMD and the source size is inferred from this comparison. Our analysis shows that, for both reactions, the characteristic size of the system at freeze-out exceeds the size of the projectile, suggesting that the fireball created in the collision has expanded. For Au+Au reactions, the observed centrality dependence of the two-proton correlation function implies that more central collisions lead to a larger source sizes.Comment: RevTex, 12 pages, 5 figure