Минимальные массы проб для анализа показателей качества сырья

Запропоновано методику пошуку мінімальної маси проби для аналізу функцій розподілу збагачувальних показників корисної копалини. На підставі розглянутої методики отримані формули, що визначають мінімальну масу проби у залежності від параметрів підготовки проби.Предложена методика поиска минимальной массы пробы для анализа функций распределения обогатительных показателей полезного ископаемого. На основании рассмотренной методики получены формулы, определяющие минимальную массу пробы в зависимости от параметров подготовки пробы.The technique of the minimum sample mass searching for the analysis of the distribution functions of mineral dressing factors was proposed. Based on the examined methods there were obtained formulas that determined the minimum sample mass as a function of the sample preparation parameters

Genetic Studies in South Balkan Populations

Within a study of the genetics of Balkan populations, four DNA-STR systems and 19 classical markers were examined in seven samples: Romanians (two groups), Albanians, Greeks and Aromuns (three groups). The results for the DNA-STR systems have been compared with data from the literature. The results show four clear separated groups: sub-Saharan black populations, North-African, Japanese and European populations. The large Balkan populations, except the Greek sample, are genetically more homogenous than the Aromun populations. A second Neighbor-joining tree based on all 23 analyzed systems, show a particular trend of the Aromun groups, which indicates a particular genetic structure

Геоэкологическая характеристика и элементный состав листьев тополя территории г. Тюмень

Работа направлена на изучение элементного состава листьев тополя как биогеохимического индикатора состояния окружающей среды городов с выраженной специализацией. Исследование проведено на примере города Тюмень.The work is directed at studying the elemental composition of poplar leaves as a biogeochemical indicator of the state of the environment of cities with a pronounced specialization. The study was conducted on the example of the city of Tyumen

Differential gene expression in 2D and lrECM cultivated cells.

<p><b>A)</b> Hierarchical cluster analysis of 2D and lrECM 3D on-top cultivated CRC cells lines. A total of 23000 transcripts were clustered. Each cell line builds an independent cluster for itself. With the exception for LOVO and HT-29, within each cell line two separate clusters were observed, due to the cultivation method of the cells: 2D versus lrECM 3D on-top. <b>B)</b> Heatmap of 225 significantly differentially regulated genes between 2D and lrECM 3D on-top cultivated cells. (Mann-Whitney-U test, <i>P</i>_corr <0.05). <b>C)</b> Literature-based network analysis of significantly differentially regulated genes (Natural Language Processor Engine, GeneSpring GX 10.5). Known interactions of 14 from a total of 225 significantly differentially regulated genes are shown. A red arrow indicates the increased expression of each gene in lrECM cultivation conditions while a green arrow indicates a reduced gene expression.</p

KEGG pathway analysis of genes distinguishing cells grown in 2D and lrECM culture conditions.

<p>Shown are all categories (pathways/hits) represented by three or more differently regulated genes when comparing 2D and lrECM 3D culture conditions.</p

New Model for Gastroenteropancreatic Large-Cell Neuroendocrine Carcinoma: Establishment of Two Clinically Relevant Cell Lines

<div><p>Recently, a novel WHO-classification has been introduced that divided gastroenteropancreatic neuroendocrine neoplasms (GEP-NEN) according to their proliferation index into G1- or G2-neuroendocrine tumors (NET) and poorly differentiated small-cell or large-cell G3-neuroendocrine carcinomas (NEC). Our knowledge on primary NECs of the GEP-system is limited due to the rarity of these tumors and chemotherapeutic concepts of highly aggressive NEC do not provide convincing results. The aim of this study was to establish a reliable cell line model for NEC that could be helpful in identifying novel druggable molecular targets. Cell lines were established from liver (NEC-DUE1) or lymph node metastases (NEC-DUE2) from large cell NECs of the gastroesophageal junction and the large intestine, respectively. Morphological characteristics and expression of neuroendocrine markers were extensively analyzed. Chromosomal aberrations were mapped by array comparative genomic hybridization and DNA profiling was analyzed by DNA fingerprinting. <i>In vitro</i> and <i>in vivo</i> tumorigenicity was evaluated and the sensitivity against chemotherapeutic agents assessed. Both cell lines exhibited typical morphological and molecular features of large cell NEC. <i>In vitro</i> and <i>in vivo</i> experiments demonstrated that both cell lines retained their malignant properties. Whereas NEC-DUE1 and -DUE2 were resistant to chemotherapeutic drugs such as cisplatin, etoposide and oxaliplatin, a high sensitivity to 5-fluorouracil was observed for the NEC-DUE1 cell line. Taken together, we established and characterized the first GEP large-cell NEC cell lines that might serve as a helpful tool not only to understand the biology of these tumors, but also to establish novel targeted therapies in a preclinical setup.</p></div