Совершенствование технологии сооружения подводных переходов трубопроводов проложенных методом горизонтально-направленного бурения

Цель работы – разработка рекомендаций по применению технологии прокладки трубопроводов методом горизонтально-направленного бурения с применением защитного устройства. В процессе исследования проводились сравнительная характеристика существующих методов бестраншейной прокладки трубопроводов, расчет основных характеристик подводного перехода, подбор защитного устройства, исследование напряженно-деформированного состояния трубопровода с помощью конечно-элементного моделирования в программном комплексе Ansys. В результате исследования подобраны оптимальные параметры подводного перехода, методом конечно-элементного моделирования в программном комплексе Ansys определено напряженно-деформированнон состояние трубопровода с защитным устройством и без него.The purpose of the work is to develop recommendations for the application of pipeline laying technology by the method of horizontal directional drilling using a protective device. In the process of the research, the existing methods of trenchless pipeline laying, the calculation of the main characteristics of the underwater crossing, the selection of the protective device, the study of the stress-strain state of the pipeline using finite element modeling in the software complex Ansys were compared. As a result of the research, optimal parameters of the underwater transition were selected, the finite element method in the software complex Ansys determined the stress-strain state of the pipeline with the protective device and without it

An experimental DUAL model of advanced liver damage

Individuals exhibiting an intermediate alcohol drinking pattern in conjunction with signs of metabolic risk present clinical features of both alcohol-associated and metabolic-associated fatty liver diseases. However, such combination remains an unexplored area of great interest, given the increasing number of patients affected. In the present study, we aimed to develop a preclinical DUAL (alcohol-associated liver disease plus metabolic-associated fatty liver disease) model in mice. C57BL/6 mice received 10% vol/vol alcohol in sweetened drinking water in combination with a Western diet for 10, 23, and 52 weeks (DUAL model). Animals fed with DUAL diet elicited a significant increase in body mass index accompanied by a pronounced hypertrophy of adipocytes, hypercholesterolemia, and hyperglycemia. Significant liver damage was characterized by elevated plasma alanine aminotransferase and lactate dehydrogenase levels, extensive hepatomegaly, hepatocyte enlargement, ballooning, steatosis, hepatic cell death, and compensatory proliferation. Notably, DUAL animals developed lobular inflammation and advanced hepatic fibrosis. Sequentially, bridging cirrhotic changes were frequently observed after 12 months. Bulk RNA-sequencing analysis indicated that dysregulated molecular pathways in DUAL mice were similar to those of patients with steatohepatitis. Conclusion: Our DUAL model is characterized by obesity, glucose intolerance, liver damage, prominent steatohepatitis and fibrosis, as well as inflammation and fibrosis in white adipose tissue. Altogether, the DUAL model mimics all histological, metabolic, and transcriptomic gene signatures of human advanced steatohepatitis, and therefore serves as a preclinical tool for the development of therapeutic targets