Studying the spatial distribution of interstellar dust

The spacial distribution of interstellar dust reflects both interstellar dynamics and the processes which form and destroy dust in the interstellar medium (ISM). The IRAS survey, because of its high sensitivity to thermal emission from dust in the IR, provides new approaches to determining the spatial distribution of dust. The initial results are reported of an attempt to use the IRAS data to probe the spatial distribution of dust - by searching for thermal emission from dust in the vicinity of bright stars. These results show that this technique (which relies on finding IR emission associated with randomly selected stars) can ultimately be used to study the distribution of dust in the ISM. The density of the cloud producing the IR emission may be derived by assuming that the dust is at its projected distance from the star and that the heating is due to the star's (known) radiation field. The heating radiation is folded into a grain model, and the number of emitting grains adjusted to reproduce the observed energy distribution. It is noted that this technique is capable in principle of detecting dust densities much lower than those typical of the cirrus clouds

The Five-Factor Model of Personality and its Relation to Physical Activity Type

Previous research has linked components of the Five-Factor model of personality to physical health and well-being. The strength of these relationships with specific exercise behaviors is, however, not yet known, which may impact how practitioners prescribe exercise. For this reason, it is important to find ways to utilize personality to provide stronger exercise prescriptions for inactive individuals. Purpose: The aim of this pilot, exploratory study was to assess the relationship between personality types and individual exercise preference. Methods: Women (N = 51) between the ages of 30 and 50 years completed a demographic survey, the IPIP Big 5 personality questionnaire, the Physical Activity Enjoyment scale, the Godin Leisure-Time Exercise questionnaire, and a Lifestyle-Related Self-Concept questionnaire. A Chi squared test was performed to determine the relationship between personality and preferred physical activity modality. Results: Participants of the study had an average age of 38.9 [plus-or-minus] 6.4 years and were 78.8% White and 17.6% Black or African American. Of the total participants, 31.4% completed an undergraduate degree and 51% completed a post-graduate education. Eighteen (35.3%) participants had an annual income below $50,0000, 27 participants (52.9$50,000 and $99,999, and 5 participants ( 9.8$100,000. The Pearson chi-square test comparing 5 different modes of exercise resulted in a value of 30.185 (p = 0.02). A second chi-square test that combined all group fitness classes and compared them with Crossfit and individual exercises resulted in a value of 16.179 (p = 0.04). Thus, personality type was significantly correlated with preferred mode of exercise. An ANOVA comparing personality scales of personality with preferred exercise resulted in a significant value for only the conscientious domain (p=.01). This suggested that conscientious individuals would prefer crossfit and aerobic based group classes. Conclusions: Personality did significantly differentiate individuals based on their preferred mode. Significant results note differences in individuals that participate in group fitness classes, Crossfit, and individual exercises. Given the proportion of the population that does not meet physical activity and public health recommendations, it would be beneficial to utilize this relationship to better prescribe tailored physical activity rather than providing overarching recommendations to individuals. An intervention prescribing physical activity based on an individual's personality type is needed to further the understanding of the relationships presented

Ultraviolet Emission Lines in Young Low Mass Galaxies at z~2: Physical Properties and Implications for Studies at z>7

We present deep spectroscopy of 17 very low mass (M* ~ 2.0x10^6 Msun to 1.4x10^9 Msun) and low luminosity (M_UV ~ -13.7 to -19.9) gravitationally lensed galaxies in the redshift range z~1.5-3.0. Deep rest-frame ultraviolet spectra reveal large equivalent width emission from numerous lines (NIV], OIII], CIV, Si III], CIII]) which are rarely seen in individual spectra of more massive star forming galaxies. CIII] is detected in 16 of 17 low mass star forming systems with rest-frame equivalent widths as large as 13.5 Angstroms. Nebular CIV emission is present in the most extreme CIII] emitters, requiring an ionizing source capable of producing a substantial component of photons with energies in excess of 47.9 eV. Photoionization models support a picture whereby the large equivalent widths are driven by the increased electron temperature and enhanced ionizing output arising from metal poor gas and stars, young stellar populations, and large ionization parameters. The young ages implied by the emission lines and continuum SEDs indicate that the extreme line emitters in our sample are in the midst of a significant upturn in their star formation activity. The low stellar masses, blue UV colors, and large sSFRs of our sample are similar to those of typical z>6 galaxies. Given the strong attenuation of Ly-alpha in z>6 galaxies we suggest that CIII] is likely to provide our best probe of early star forming galaxies with ground-based spectrographs and one of the most efficient means of confirming z>10 galaxies with the James Webb Space Telescope.Comment: 22 pages, 8 figures, accepted for publication in MNRA

Implementation of a screening program for patients at risk for posttraumatic stress disorder

Introduction Implantable cardioverter defibrillator (ICD) recipients who suffer from posttraumatic stress disorder (PTSD) are known to be associated with significant cardiac-specific mortality. Clinical observations suggest that PTSD is frequently undetected in ICD recipients followed up at electrophysiology (EP) outpatient clinics. Early recognition of PTSD is important to reduce the risk of serious manifestations on patient outcomes. Methods All ICD recipients aged 19 years or older at the Washington University School of Medicine (WASHU) EP clinic, a large urban EP clinic, were invited to participate in the project. An informed consent letter with an attached primary care: posttraumatic stress disorder (PC: PTSD) survey was offered to the participants who met the inclusion criteria. Those who completed the survey were included in the project. Individuals with positive survey result were offered a referral to mental health services. Comparisons between PTSD and non-PTSD patients were done using a two-sample t -test for continuous variables. Using Fisher's exact test, PTSD prevalence was compared to the study by Ladwig et al in which prevalence was determined as the proportion of patients with positive findings of PTSD ( n = 38/147). All analyses were conducted using SAS v9.4. The proportion of patients having PTSD was determined and an exact 95% confidence interval was evaluated based on the binomial distribution. Results Using a convenience sample, 50 ICD recipients (33 males and 17 females) were enrolled. The project had a 30-day outcome period. Nine (18%) of the 50 participants had positive PC: PTSD findings and all these nine participants were referred to a mental health specialist. The current project demonstrated an 18% (9/50) PTSD prevalence rate when compared to a 26% (38/147) prevalence rate in the study by Ladwig et al ( P = 0.34). Although this project did not demonstrate 20% PTSD prevalence rate, as hypothesized, the 18% PTSD prevalence rate is consistent with previous research. Conclusion The prevalence of PTSD noted in the current project is consistent with previous research and validates underrecognition of PTSD in ICD patients. Offering a referral to all ICD recipients at EP clinic visits with a positive PC: PTSD screening to a mental health specialist is an important step in reducing the risk of serious manifestations on patient outcomes

Pyrophosphate-Dependent ATP Formation from Acetyl Coenzyme A in Syntrophus aciditrophicus, a New Twist on ATP Formation.

UnlabelledSyntrophus aciditrophicus is a model syntrophic bacterium that degrades key intermediates in anaerobic decomposition, such as benzoate, cyclohexane-1-carboxylate, and certain fatty acids, to acetate when grown with hydrogen-/formate-consuming microorganisms. ATP formation coupled to acetate production is the main source for energy conservation by S. aciditrophicus However, the absence of homologs for phosphate acetyltransferase and acetate kinase in the genome of S. aciditrophicus leaves it unclear as to how ATP is formed, as most fermentative bacteria rely on these two enzymes to synthesize ATP from acetyl coenzyme A (CoA) and phosphate. Here, we combine transcriptomic, proteomic, metabolite, and enzymatic approaches to show that S. aciditrophicus uses AMP-forming, acetyl-CoA synthetase (Acs1) for ATP synthesis from acetyl-CoA. acs1 mRNA and Acs1 were abundant in transcriptomes and proteomes, respectively, of S. aciditrophicus grown in pure culture and coculture. Cell extracts of S. aciditrophicus had low or undetectable acetate kinase and phosphate acetyltransferase activities but had high acetyl-CoA synthetase activity under all growth conditions tested. Both Acs1 purified from S. aciditrophicus and recombinantly produced Acs1 catalyzed ATP and acetate formation from acetyl-CoA, AMP, and pyrophosphate. High pyrophosphate levels and a high AMP-to-ATP ratio (5.9 ± 1.4) in S. aciditrophicus cells support the operation of Acs1 in the acetate-forming direction. Thus, S. aciditrophicus has a unique approach to conserve energy involving pyrophosphate, AMP, acetyl-CoA, and an AMP-forming, acetyl-CoA synthetase.ImportanceBacteria use two enzymes, phosphate acetyltransferase and acetate kinase, to make ATP from acetyl-CoA, while acetate-forming archaea use a single enzyme, an ADP-forming, acetyl-CoA synthetase, to synthesize ATP and acetate from acetyl-CoA. Syntrophus aciditrophicus apparently relies on a different approach to conserve energy during acetyl-CoA metabolism, as its genome does not have homologs to the genes for phosphate acetyltransferase and acetate kinase. Here, we show that S. aciditrophicus uses an alternative approach, an AMP-forming, acetyl-CoA synthetase, to make ATP from acetyl-CoA. AMP-forming, acetyl-CoA synthetases were previously thought to function only in the activation of acetate to acetyl-CoA

LEGUS and Halpha-LEGUS Observations of Star Clusters in NGC 4449: Improved Ages and the Fraction of Light in Clusters as a Function of Age

We present a new catalog and results for the cluster system of the starburst galaxy NGC 4449 based on multi-band imaging observations taken as part of the LEGUS and Halpha-LEGUS surveys. We improve the spectral energy fitting method used to estimate cluster ages and find that the results, particularly for older clusters, are in better agreement with those from spectroscopy. The inclusion of Halpha measurements, the role of stochasticity for low mass clusters, the assumptions about reddening, and the choices of SSP model and metallicity all have important impacts on the age-dating of clusters. A comparison with ages derived from stellar color-magnitude diagrams for partially resolved clusters shows reasonable agreement, but large scatter in some cases. The fraction of light found in clusters relative to the total light (i.e., T_L) in the U, B, and V filters in 25 different ~kpc-size regions throughout NGC 4449 correlates with both the specific Region Luminosity, R_L, and the dominant age of the underlying stellar population in each region. The observed cluster age distribution is found to decline over time as dN/dt ~ t^g, with g=-0.85+/-0.15, independent of cluster mass, and is consistent with strong, early cluster disruption. The mass functions of the clusters can be described by a power law with dN/dM ~ M^b and b=-1.86+/-0.2, independent of cluster age. The mass and age distributions are quite resilient to differences in age-dating methods. There is tentative evidence for a factor of 2-3 enhancement in both the star and cluster formation rate ~100 - 300 Myr ago, indicating that cluster formation tracks star formation generally. The enhancement is probably associated with an earlier interaction event

Sampling Local Fungal Diversity in an Undergraduate Laboratory using DNA Barcoding

Traditional methods for fungal species identification require diagnostic morphological characters and are often limited by the availability of fresh fruiting bodies and local identification resources. DNA barcoding offers an additional method of species identification and is rapidly developing as a critical tool in fungal taxonomy. As an exercise in an undergraduate biology course, we identified 9 specimens collected from the Hendrix College campus in Conway, Arkansas, USA to the genus or species level using morphology. We report that DNA barcoding targeting the internal transcribed spacer (ITS) region supported several of our taxonomic determinations and we were able to contribute 5 ITS sequences to GenBank that were supported by vouchered collection information. We suggest that small-scale barcoding projects are possible and that they have value for documenting fungal diversity