Dodatek kwartalny [Finanse i Prawo Finansowe • Journal of Finance and Financial Law • Kwartalnik • 2015, II(1)]

Tomasz Uryszek - Zadłużenie publiczne w 2014 roku • Artur Zimny - Koniunktura w sektorze przedsiębiorstw niefinansowych w 4. kwartale 2014 r. • Artur Zimny - Koniunktura giełdowa • Radosław Witczak - Zmiany w świecie finansów (podatków) • Artur Gądek, Kornel Kot - Sytuacja gospodarcza w Polsce po pierwszym kwartale 2015 r. • Iwa Kuchciak - Polski sektor bankowy wobec wzrostu ryzyka walutowego • Magdalena Ślebocka, Aneta Tylman - Rewitalizacja miast polskich – zagadnienia wstępne. Projekt Wytycznych w zakresie finansowania rewitalizacji w programach operacyjnych na lata 2014–202

PPE51 Is Involved in the Uptake of Disaccharides by Mycobacterium tuberculosis

We have recently found that selected thio-disaccharides possess bactericidal effects against Mycobacterium tuberculosis but not against Escherichia coli or Staphylococcus aureus. Here, we selected spontaneous mutants displaying resistance against the investigated thio-glycoside. According to next-generation sequencing, four of six analyzed mutants which were resistant to high concentrations of the tested chemical carried nonsynonymous mutations in the gene encoding the PPE51 protein. The complementation of these mutants with an intact ppe51 gene returned their sensitivity to the wild-type level. The uptake of tritiated thio-glycoside was significantly more abundant in wild-type Mycobacterium tuberculosis compared to the strain carrying the mutated ppe51 gene. The ppe51 mutations or CRISPR-Cas9-mediated downregulation of PPE51 expression affected the growth of mutant strains on minimal media supplemented with disaccharides (maltose or lactose) but not with glycerol or glucose as the sole carbon and energy source. Taking the above into account, we postulate that PPE51 participates in the uptake of disaccharides by tubercle bacilli