Электроснабжение Бухарского нефтеперерабатывающего завода

Объектом исследования является электрическая часть Бухарского нефтеперерабатывающего завода. Цель работы – проектирование схемы электроснабжения предприятия, выбор оборудования. В работе спроектирована схема электроснабжения от подстанции энергосистемы, до конечного электроприемника, выбраны кабели и провода, коммутационное оборудование, были сделаны необходимые проверки.Object of a research is the electric part of the Bukhara oil refinery. The work purpose – projection of the scheme of power supply of the enterprise, the choice of an inventory. In work the scheme of power supply from substation of a power supply system, to the terminating electroreceiver is designed, cables and wires, a switching inventory are chosen, necessary checks were made

Разработка проекта виртуального клуба путешествий мам с детьми «Милым Малым»

Цель выпускной квалификационной работы – разработать проект виртуального клуба путешествий мам с детьми "МилымМалым". Проблему исследования можно сформулировать в вопросе, как использовать возможности виртуального туризма для обеспечения информационной поддержки путешествий мам с детьми? В результате исследования: 1.Разработан механизм дифференциации такой сложной и многосегментной целевой аудитории как мамы с детьми. 2.Предложена методика составления карты эмпатии для конкретного сегмента данной целевой аудитории. 3.Разработан алгоритм выявления туристских потребностей мам с детьми. 4.Показана необходимость предварить реализацию проекта виртуального клуба путешествий мам с детьми обеспечением информационной поддержки.The goal of the final qualifying work is to develop a project for a virtual club for traveling with mothers and children, "MilymMalym". The research problem can be formulated in the question of how to use the opportunities of virtual tourism to provide information support for mothers traveling with children? As a result of the research: 1. A mechanism for differentiating such a complex and multi-segment target audience as mothers with children has been developed. 2. The methodology of drawing a map of empathy for a particular segment of this target audience is proposed. 3. An algorithm for identifying the tourist needs of mothers with children has been developed. 4. It is shown the need to precede the implementation of the project of a virtual traveling club for mothers and children provi

Research on Layer Manufacturing Techniques at Fraunhofer

Within the German Fraunhofer-Gesellschaft, the Fraunhofer Alliance Rapid Prototyping unites the competences of 12 institutes in the field of solid freeform fabrication. Covered competences are virtual and computer-aided product planning methods and techniques, the development and integration of materials and processes for different industrial sectors. This paper presents actual research results on layer manufacturing within the Fraunhofer- Gesellschaft based on examples from Fraunhofer ILT »Laser Melting - Direct manufacturing of metal parts with unique properties«, Fraunhofer IFAM »ecoMold - A novel concept to produce molds for plastic injection molding and pressure die casting« and Fraunhofer IPT »Quick manufacture, repair and modification of steel molds using Controlled Metal Build Up (CMB)«.Mechanical Engineerin

Dissipation of Proton Motive Force is not Sufficient to Induce the Phage Shock Protein Response in Escherichia coli

Phage shock proteins (Psp) and their homologues are found in species from the three domains of life: Bacteria, Archaea and Eukarya (e.g. higher plants). In enterobacteria, the Psp response helps to maintain the proton motive force (PMF) of the cell when the inner membrane integrity is impaired. The presumed ability of ArcB to sense redox changes in the cellular quinone pool and the strong decrease of psp induction in ΔubiG or ΔarcAB backgrounds suggest a link between the Psp response and the quinone pool. The authors now provide evidence indicating that the physiological signal for inducing psp by secretin-induced stress is neither the quinone redox state nor a drop in PMF. Neither the loss of the H+-gradient nor the dissipation of the electrical potential alone is sufficient to induce the Psp response. A set of electron transport mutants differing in their redox states due to the lack of a NADH dehydrogenase and a quinol oxidase, but retaining a normal PMF displayed low levels of psp induction inversely related to oxidised ubiquinone levels under microaerobic growth and independent of PMF. In contrast, cells displaying higher secretin induced psp expression showed increased levels of ubiquinone. Taken together, this study suggests that not a single but likely multiple signals are needed to be integrated to induce the Psp response

Osmosensitivity of Transient Receptor Potential Vanilloid 1 Is Synergistically Enhanced by Distinct Activating Stimuli Such as Temperature and Protons

In animals, body-fluid osmolality is continuously monitored to keep it within a narrow range around a set point (∼300 mOsm/kg). Transient receptor potential vanilloid 1 (TRPV1), a cation channel, has been implicated in body-fluid homeostasis in vivo based on studies with the TRPV1-knockout mouse. However, the response of TRPV1 to hypertonic stimuli has not been demonstrated with heterologous expression systems so far, despite intense efforts by several groups. Thus, the molecular entity of the hypertonic sensor in vivo still remains controversial. Here we found that the full-length form of TRPV1 is sensitive to an osmotic increase exclusively at around body temperature using HEK293 cells stably expressing rat TRPV1. At an ambient temperature of 24°C, a slight increase in the intracellular calcium concentration ([Ca2+]i) was rarely observed in response to hypertonic stimuli. However, the magnitude of the osmosensitive response markedly increased with temperature, peaking at around 36°C. Importantly, the response at 36°C showed a robust increase over a hypertonic range, but a small decrease over a hypotonic range. A TRPV1 antagonist, capsazepine, and a nonspecific TRP channel inhibitor, ruthenium red, completely blocked the increase in [Ca2+]i. These results endorse the view that the full-length form of TRPV1 is able to function as a sensor of hypertonic stimuli in vivo. Furthermore, we found that protons and capsaicin likewise synergistically potentiated the response of TRPV1 to hypertonic stimuli. Of note, HgCl2, which blocks aquaporins and inhibits cell-volume changes, significantly reduced the osmosensitive response. Our findings thus indicate that TRPV1 integrates multiple different types of activating stimuli, and that TRPV1 is sensitive to hypertonic stimuli under physiologically relevant conditions

TRPV6 Determines the Effect of Vitamin D3 on Prostate Cancer Cell Growth

Despite remarkable advances in the therapy and prevention of prostate cancer it is still the second cause of death from cancer in industrialized countries. Many therapies initially shown to be beneficial for the patients were abandoned due to the high drug resistance and the evolution rate of the tumors. One of the prospective therapeutical agents even used in the first stage clinical trials, 1,25-dihydroxyvitamin D3, was shown to be either unpredictable or inefficient in many cases. We have already shown that TRPV6 calcium channel, which is the direct target of 1,25-dihydroxyvitamin D3 receptor, positively controls prostate cancer proliferation and apoptosis resistance (Lehen'kyi et al., Oncogene, 2007). However, how the known 1,25-dihydroxyvitamin D3 antiproliferative effects may be compatible with the upregulation of pro-oncogenic TRPV6 channel remains a mystery. Here we demonstrate that in low steroid conditions 1,25-dihydroxyvitamin D3 upregulates the expression of TRPV6, enchances the proliferation by increasing the number of cells entering into S-phase. We show that these pro-proliferative effects of 1,25-dihydroxyvitamin D3 are directly mediated via the overexpression of TRPV6 channel which increases calcium uptake into LNCaP cells. The apoptosis resistance of androgen-dependent LNCaP cells conferred by TRPV6 channel is drastically inversed when 1,25-dihydroxyvitamin D3 effects were combined with the successful TRPV6 knockdown. In addition, the use of androgen-deficient DU-145 and androgen-insensitive LNCaP C4-2 cell lines allowed to suggest that the ability of 1,25-dihydroxyvitamin D3 to induce the expression of TRPV6 channel is a crucial determinant of the success or failure of 1,25-dihydroxyvitamin D3-based therapies

Interdomain Interactions Control Ca2+-Dependent Potentiation in the Cation Channel TRPV4

Several Ca2+-permeable channels, including the non-selective cation channel TRPV4, are subject to Ca2+-dependent facilitation. Although it has been clearly demonstrated in functional experiments that calmodulin (CaM) binding to intracellular domains of TRP channels is involved in this process, the molecular mechanism remains elusive. In this study, we provide experimental evidence for a comprehensive molecular model that explains Ca2+-dependent facilitation of TRPV4. In the resting state, an intracellular domain from the channel N terminus forms an autoinhibitory complex with a C-terminal domain that includes a high-affinity CaM binding site. CaM binding, secondary to rises in intracellular Ca2+, displaces the N-terminal domain which may then form a homologous interaction with an identical domain from a second subunit. This represents a novel potentiation mechanism that may also be relevant in other Ca2+-permeable channels

A Development Consensus reconciling the Beijing Model and Washington Consensus: Views and Agenda

Reconciling the two dominant development models of the Washington Consensus (WC) and Beijing Model (BM) remains a critical challenge in the literature. The challenge is even more demanding when emerging development paradigms like the Liberal Institutional Pluralism (LIP) and New Structural Economics (NSE) schools have to be integrated. While the latter has recognized both State and market failures but failed to provide a unified theory, the former has left the challenging concern of how institutional diversity matter in the development process. We synthesize perspectives from over 150 recently published papers on development and Sino-African relations in order to present the relevance of both the WC and BM in the long-term and short-run respectively. While the paper provides a unified theory by reconciling the WC and the BM to complement the NSE, it at the same time presents a case for economic rights and political rights as short-run and long-run development priorities respectively. By reconciling the WC with the BM, the study contributes at the same to macroeconomic NSE literature of unifying a development theory and to the LIP literature on institutional preferences with stages of development. Hence, the proposed reconciliation takes into account the structural and institutional realities of nations at difference stages of the process of development