Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation

Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. These datasets accompany "Filter Aided Sample Preparation - A Tutorial" (Wisniewski, 2019). (C) 2019 The Authors. Published by Elsevier Inc

The shape of asteroid 1917 Cuyo

Lightcurves obtained for 1917 Cuyo at solar phase angles near 54 degrees have an amplitude delta m = 0.44 mag. However, convex-profile inversion of the lightcurves yields an estimate of the asteroid's mean cross section (C, a 2-D average of the 3-D shape) that is only slightly noncircular, with an elongation approximately 1.15. The estimate of C undoubtedly contains systematic errors, the most severe of which could arise from non-equatorial viewing/illumination geometry. However, Cuyo's radar echo shows very little variation in bandwidth vs. rotation phase, supporting the hypothesis that this asteroid's elongation is rather modest

Preliminary Note on a Correlation of a Boundary-Layer Transition Results on Highly Cooled Blunt Bodies

Transition data on highly cooled blunt bodies are correlated in terms of the ratio of wall to local-stream enthalpy, Reynolds number based on displacement thickness, and location of transition. The proposed correlation, although not sensitive enough to predict the exact location of transition does predict the enthalpy ratio below which very early transition on blunt bodies is expected. The correlation is not altered by moderate amounts of surface roughness; however, the location of transition may well be affected by roughness

Relating the biogeochemistries of zinc, cobalt, and phosphorus to phytoplankton activities in the sea

Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution June, 2006This thesis explores the potential of zinc, cobalt, and phosphorus to influence primary production in the subarctic North Pacific, the Bering Sea, and the North Atlantic Ocean, In the North Pacific and Bering Sea, total zinc concentrations were measured along a near-surface transect and in selected deep profiles. Zinc speciation was also measured with a novel anodic stripping voltammetry method, and the results were consistent with previous studies using different methods. The potential for zinc to impact primary production in the North Pacific was demonstrated in a shipboard incubation and by comparing two phytoplankton pigment markers to total zinc and free zinc ion concentrations. In the North Atlantic, total dissolved zinc and cobalt concentrations were measured and compared to concentrations of dissolved inorganic phosphorus and chlorophyll. In some areas of the North Atlantic the concentrations of zinc and cobalt were decoupled. The relationship between cobalt and inorganic phosphorus suggests that cobalt drawdown may be related to a high alkaline phosphatase related demand at low phosphorus concentrations. This trend compliments a shipboard incubation where alkaline phosphatase activities increased after cobalt addition. The presence of measurable alkaline phosphatase activity indicated that the phytoplankton community in the Sargasso Sea was experiencing phosphorus stress. Shipboard incubations generally confirmed this with inorganic phosphorus additions resulting in chlorophyll increases at 4 out of 5 stations. Further, the addition of dissolved organic phosphorus, as either a phosphate monoester or a phosphonate compound, resulted in a chlorophyll increase in 3 out of 3 incubations. This suggests that dissolved organic phosphorus may be an important phosphorus source for phytoplankton in low phosphorus environments and that the ability to use phosphonates may be more widespread than previously recognized. Overall, this thesis adds to our understanding of how the nutrients phosphorus, zinc, and cobalt may influence primary production.I received funding towards my graduate research from the Richard Vanstone Summer Fellowship, the Stanley W. Watson Student Fellowship, the Arch Scurlock Fund for research, the Center for Environmental Bioinorganic Chemistry, the EPA STAR Fellowship, the National Science Foundation (OCE-0136835), and the WHO1 Academic Programs Office