Keragaman Genetik Rusa Sambar (Rusa Unicolor), Pemanfaatan Dan Implikasinya Untuk Konservasi

Genetic Diversity of Sambar Deer (Rusa unicolor) in Utilization and Implications for Conservation. The deer sambar (Rusa unicolor) is one of species of Genus Rusa, was widely distributed in South Asia antil South East Asia. This species in Indonesia was widely distributed in Kalimantan and Sumatra island, but now, the hunting, poaching and habitat loss have reduced its populations drasstically. In order to provide useful information for its conservations. The genetic diversity and population structure of the wild sambar deer was observed by analyzing the 962 bp long of fragment mitochondrial DNA (mtDNA) control region (D-loop)fragment. The result detected, twenty different haplotypes from 22 samples from Borneo and Sumatra. Overall, sambar deer have a relatively high genetic diversity compared to other the ceervid species, with a haplotype diversity (h) 0.9870 and nucleotide diversity (ð) 2.931±0.260%.The genetic distance of Borneo populations (East Borneo) higher compared with Sumatra's populations that is d = 0.018±0.003 on Borneo and d = 0.009±0.002 on Sumatra. The structure of phylogenetic tree showed that two populations were separate based on the haplotypes differences

Observations on Natural Foods and Nutrition Content of Critically Endangered Turtle (Leucocephalon yuwonoi) in Central Sulawesi

Kura-kura Sulawesi (Leucocephalon yuwonoi) adalah satu dari dua jenis kura-kura endemik Sulawesi dengan penyebaran yang terbatas. Sejauh ini data natural historinya sangat minim, padahal sangat dibutuhkan dalam upaya penangkaran satwa yang berstatus kritis IUCN ini. Penelitian ini bertujuan untuk mengungkap tentang pakan di alam beserta kandungan nutrisinya. Survei lapangan dilakukan di kawasan Bangkir dari tanggal 23 Mei sampai 5 Juni 2004 dan di Moutong dari tanggal 11 sampai 19 Oktober 2004. Data pakan di alam diperoleh dari analisis sampel feces dan hasil wawancara kepada para pemburu kura-kura beserta pengumpul. Kepastian nama jenis pakan diperoleh dengan melakukan identifikasi sampel tumbuhan pakan di Herbarium Bogoriense. Data kandungan nutrisi diperoleh melalui analisis proksimat sampel pakan yang terkoleksi selama survei. Hasil penelitian ini menunjukkan bahwa terdapat 32 jenis tumbuhan yang jadi sumber pakan di alam, variasi kandungan nutrisi yang cukup besar, kandungan lemak rendah berkisar antara 0.74 - 8.33% (2.41+2.03) dari berat kering. Berdasarkan dominansi, keberadaan di habitat (multiple season) dan tingginya kandungan energi diduga kuat bahwa Colocasia esculenta, Limnocharis flava and Ipomoea aquatica merupakan pakan utama di alam

Penanda Genetik Tarsius (Tarsius Spp.) Dengan Menggunakan Gen Cytochrome Oxidase I (COI) DNA Mitokondria (MtDNA) Melalui Metode Sekuensing

Tarsier (Tarsius spp.) are the smallest primates in the world. Currently there are 10 species, of which 9 species are distributed in Indonesia and 8 species of them are endemic to Sulawesi. Morphologically the Sulawesi spesies are almost similar. This research is aimed to identify the use of Cytochrome Oxidase I (COI) gene as a genetic marker on Tarsius spp. for conservation purposes. Sixteen individuals consisted of 10 Tarsius bancanus, 4 Tarsius sp., 1 Tarsius wallacei , and 1 Tarsius sangirensis were collected from various places and analysed using COI gene. The results showed there were 238 different sites of nucleotides and 159 sites of amino acids from the total amount of 838 bp. The genetic distance by Kimura-2 parameter showed the highest value was 26% while the lowest was 0%. The average genetic distance was 11,5%. Phylogenetic tree constructed by Neighbour-Joining method based on nucleotides sequence showed that the COI gene could be used as a genetic marker to differentiate among Tarsius spp. but could not be used as a clear marker for tarsiers in Sulawesi. Based on the analysis, there is a high value of genetic variation among Tarsius spp. with much lower genetic variation in Western Tarsier population compared to Eastern Tarsier

Variasi Genetik pada Rusa Sambar (Rusa unicolor) di Penangkaran, Kabupaten Penajam, Kalimantan Timur

Sambar deer (Rusa unicolor) is the largest Indonesian deer species. The largest population of captivation is located at Penajam district, East Borneo (UPTD). First population was introduced in 1990 with four individuals. The use of molecular marker was aimed to identify and characterise the level of genetic diversity within the UPTD population as well as to identify a possible of botolneck population genetic status. This study discussed the relevance of the result for management purposes of captivation. The results indicated that sambar deer populatin (n=38) had an average genetic distance (d) in population as 0.006 with nucleotide diversity (π) being 0.0159). A total of 43.48% of the population was homogeneous that showed no nucleotide differences among individuals

An exploration on the habitat, feeds and distribution of Tarsius tarsier (tarsier) in Selayar Island and Patunuang Nature Reserve, South Sulawesi

This research was conducted in the forest of Selayar Island and in the Nature Reserve on forest of Patunuang South Sulawesi. The study sites were located at 0-250 m above sea level on four locations. Data being collected were the distribution, type of feeds, nest sites, habitat utilization and of type. During the observation, we found at least 10 nest sites which were distributed on three locations. The tarsier consume mainly insect, and it isn’t collected. Fifty two species from 31 families were identified being habitat and five species from three families were the nest of Tarsier which were dominated by bamboo

Reproductive Organ of Male Sulawesi Giant Rat (Paruromys Dominator)

Indonesia has a biodiversity in fauna and one of them is the rat. The methodology to identify animal character is mostly based on morphological structure, the example of body lenght and hair color. However, reproductive organs of every species are diferent in location, shape, color and weight. Paruromys dominator is one of rats species in Sulawesi. Although, many informations about the rat\u27s morphology is already provided , but no data about reproductive organ reported yet. Knowing, identifying and understanding the reproductive organs in that species must be done by sampling reproductive organs and measure all of them,. All of the reproductive organs were then collected for anatomical sample and processed for histological staining and were analyzed with light microscope and SEM. Based on the present study, Paruromys dominator has reproductive organs which are like other murids, namely seminal vesicle, testis, prostat glands, Cowper\u27s glans and glans penis. There is no diference about histological stucture of rat\u27s testis with other rats species, but based on the result of SEM, the penis has many penile spines. The sperm head is hook shape, it is like Rattus norvegicus. The average of head length and tail lenght of sperm are 1,28±0,101 μm and 12,95±1,139 μm, respectively

Keragaman Genetik pada Kukang (Nycticebus Coucang) Berdasarkan pada Gen 12S RRNA Mitokondria

Genetic Diversity of Slow loris (Nycticebus coucang) based on Mitochondrial 12S rRNA gene. The research on genetic diversity of slow loris Nycticebus coucang (kukang) was carried out. The samples are 12 individuals from three locations (Sumedang and Jember in Java, and Lampung in Sumatera). Total DNA was extracted from blood and tissue. The mitochondrial 12S rRNA nucleotide sequences were determined to investigate genetic diversity of this species. This region was amplified by using L1091 and H 1478 primers by PCR. As a result of the analysis for 386 bp nucleotide sequence, five haplotypes were found, two from Java and three from Sumatra, respectively