2,237 research outputs found
‘I went with what I always do…’:a qualitative analysis of ‘Cleggmania’ and vote choice in the 2010 British General Election
Analysis of nanopore detector measurements using Machine-Learning methods, with application to single-molecule kinetic analysis
<p>Abstract</p> <p>Background</p> <p>A nanopore detector has a nanometer-scale trans-membrane channel across which a potential difference is established, resulting in an ionic current through the channel in the pA-nA range. A distinctive channel current blockade signal is created as individually "captured" DNA molecules interact with the channel and modulate the channel's ionic current. The nanopore detector is sensitive enough that nearly identical DNA molecules can be classified with very high accuracy using machine learning techniques such as Hidden Markov Models (HMMs) and Support Vector Machines (SVMs).</p> <p>Results</p> <p>A non-standard implementation of an HMM, emission inversion, is used for improved classification. Additional features are considered for the feature vector employed by the SVM for classification as well: The addition of a single feature representing spike density is shown to notably improve classification results. Another, much larger, feature set expansion was studied (2500 additional features instead of 1), deriving from including all the HMM's transition probabilities. The expanded features can introduce redundant, noisy information (as well as diagnostic information) into the current feature set, and thus degrade classification performance. A hybrid Adaptive Boosting approach was used for feature selection to alleviate this problem.</p> <p>Conclusion</p> <p>The methods shown here, for more informed feature extraction, improve both classification and provide biologists and chemists with tools for obtaining a better understanding of the kinetic properties of molecules of interest.</p
Defense against predators incurs high reproductive costs for the aposematic moth<i> Arctia plantaginis</i>
Observations of Binary Stars with the Differential Speckle Survey Instrument. V. Toward an Empirical Metal-Poor Mass-Luminosity Relation
In an effort to better understand the details of the stellar structure and
evolution of metal poor stars, the Gemini North telescope was used on two
occasions to take speckle imaging data of a sample of known spectroscopic
binary stars and other nearby stars in order to search for and resolve close
companions. The observations were obtained using the Differential Speckle
Survey Instrument, which takes data in two filters simultaneously. The results
presented here are of 90 observations of 23 systems in which one or more
companions was detected, and 6 stars where no companion was detected to the
limit of the camera capabilities at Gemini. In the case of the binary and
multiple stars, these results are then further analyzed to make first orbit
determinations in five cases, and orbit refinements in four other cases. Mass
information is derived, and since the systems span a range in metallicity, a
study is presented that compares our results with the expected trend in total
mass as derived from the most recent Yale isochrones as a function of metal
abundance. These data suggest that metal-poor main-sequence stars are less
massive at a given color than their solar-metallicity analogues in a manner
consistent with that predicted from the theory
Dust-driven Winds and Mass Loss of C-rich AGB Stars with subsolar Metallicities
We investigate the mass loss of highly evolved, low- and intermediate mass
stars and stellar samples with subsolar metallicity. We give a qualitative as
well as quantitative description which can be applied to LMC/SMC-type stellar
populations. For that purpose we apply the same approach as we did for solar
metallicity stars and calculate hydrodynamical wind models including dust
formation with LMC and SMC abundances under consideration of an adapted model
assumption. In particular, we improved the treatment of the radiative transfer
problem in order to accommodate larger non-local contributions occurring with
smaller opacities. For each wind model we determine an averaged mass-loss rate.
The resulting, approximate mass-loss formulae are then applied to well-tested
and calibrated stellar evolution calculations in order to quantify the stellar
mass loss. The dynamical models for LMC and SMC metallicity result in mass-loss
rates of the same order of magnitude as the solar metallicity models which is
in this basic approach in agreement with observations. The hydrodynamical
properties like e.g. the outflow velocity differ (for fixed C/O abundance
ratio) noticeably, though. While critical luminosities of LMC and solar
metallicity models fairly coincide, the SMC models need higher luminosities to
develop dust-driven winds.Comment: 8 pages, 4 figures. Accepted for publication in A&
The α-Hemolysin nanopore transduction detector – single-molecule binding studies and immunological screening of antibodies and aptamers
A Submillimeter HCN Laser in IRC+10216
We report the detection of a strong submillimeter wavelength HCN laser line
at a frequency near 805 GHz toward the carbon star IRC+10216. This line, the
J=9-8 rotational transition within the (04(0)0) vibrationally excited state, is
one of a series of HCN laser lines that were first detected in the laboratory
in the early days of laser spectroscopy. Since its lower energy level is 4200 K
above the ground state, the laser emission must arise from the inner part of
IRC+10216's circumstellar envelope. To better characterize this environment, we
observed other, thermally emitting, vibrationally excited HCN lines and find
that they, like the laser line, arise in a region of temperature approximately
1000 K that is located within the dust formation radius; this conclusion is
supported by the linewidth of the laser. The (04(0)0), J=9-8 laser might be
chemically pumped and may be the only known laser (or maser) that is excited
both in the laboratory and in space by a similar mechanism.Comment: 11 pages, 3 figure
National Mesothelioma Virtual Bank: A standard based biospecimen and clinical data resource to enhance translational research
Background: Advances in translational research have led to the need for well characterized biospecimens for research. The National Mesothelioma Virtual Bank is an initiative which collects annotated datasets relevant to human mesothelioma to develop an enterprising biospecimen resource to fulfill researchers' need. Methods: The National Mesothelioma Virtual Bank architecture is based on three major components: (a) common data elements (based on College of American Pathologists protocol and National North American Association of Central Cancer Registries standards), (b) clinical and epidemiologic data annotation, and (c) data query tools. These tools work interoperably to standardize the entire process of annotation. The National Mesothelioma Virtual Bank tool is based upon the caTISSUE Clinical Annotation Engine, developed by the University of Pittsburgh in cooperation with the Cancer Biomedical Informatics Grid™ (caBIG™, see http://cabig.nci.nih.gov). This application provides a web-based system for annotating, importing and searching mesothelioma cases. The underlying information model is constructed utilizing Unified Modeling Language class diagrams, hierarchical relationships and Enterprise Architect software. Result: The database provides researchers real-time access to richly annotated specimens and integral information related to mesothelioma. The data disclosed is tightly regulated depending upon users' authorization and depending on the participating institute that is amenable to the local Institutional Review Board and regulation committee reviews. Conclusion: The National Mesothelioma Virtual Bank currently has over 600 annotated cases available for researchers that include paraffin embedded tissues, tissue microarrays, serum and genomic DNA. The National Mesothelioma Virtual Bank is a virtual biospecimen registry with robust translational biomedical informatics support to facilitate basic science, clinical, and translational research. Furthermore, it protects patient privacy by disclosing only de-identified datasets to assure that biospecimens can be made accessible to researchers. © 2008 Amin et al; licensee BioMed Central Ltd
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