New Uses for Sensitivity Analysis: How Different Movement Tasks Effect Limb Model Parameter Sensitivity

Original results for a newly developed eight-order nonlinear limb antagonistic muscle model of elbow flexion and extension are presented. A wider variety of sensitivity analysis techniques are used and a systematic protocol is established that shows how the different methods can be used efficiently to complement one another for maximum insight into model sensitivity. It is explicitly shown how the sensitivity of output behaviors to model parameters is a function of the controller input sequence, i.e., of the movement task. When the task is changed (for instance, from an input sequence that results in the usual fast movement task to a slower movement that may also involve external loading, etc.) the set of parameters with high sensitivity will in general also change. Such task-specific use of sensitivity analysis techniques identifies the set of parameters most important for a given task, and even suggests task-specific model reduction possibilities

Model simulation studies to clarify the effect on saccadic eye movements of initial condition velocities set by the Vestibular Ocular Reflex (VOR)

Voluntary active head rotations produced vestibulo-ocular reflex eye movements (VOR) with the subject viewing a fixation target. When this target jumped, the size of the refixation saccades were a function of the ongoing initial velocity of the eye. Saccades made against the VOR were larger in magnitude. Simulation of a reciprocally innervated model eye movement provided results comparable to the experimental data. Most of the experimental effect appeared to be due to linear summation for saccades of 5 and 10 degree magnitude. For small saccades of 2.5 degrees, peripheral nonlinear interaction of state variables in the neuromuscular plant also played a role as proven by comparable behavior in the simulated model with known controller signals

CO and HI observations of an enigmatic cloud

An isolated HI cloud with peculiar properties has recently been discovered by Dedes, Dedes, & Kalberla (2008, A&A, 491, L45) with the 300-m Arecibo telescope, and subsequently imaged with the VLA. It has an angular size of ~6', and the HI emission has a narrow line profile of width ~ 3 km/s. We explore the possibility that this cloud could be associated with a circumstellar envelope ejected by an evolved star. Observations were made in the rotational lines of CO with the IRAM-30m telescope, on three positions in the cloud, and a total-power mapping in the HI line was obtained with the Nancay Radio Telescope. CO was not detected and seems too underabundant in this cloud to be a classical late-type star circumstellar envelope. On the other hand, the HI emission is compatible with the detached-shell model that we developed for representing the external environments of AGB stars. We propose that this cloud could be a fossil circumstellar shell left over from a system that is now in a post-planetary-nebula phase. Nevertheless, we cannot rule out that it is a Galactic cloud or a member of the Local Group, although the narrow line profile would be atypical in both cases.Comment: Accepted for publication in Astronomy and Astrophysic

Circumstellar HI and CO around the carbon stars V1942 Sgr and V CrB

Context. The majority of stars that leave the main sequence are undergoing extensive mass loss, in particular during the asymptotic giant branch (AGB) phase of evolution. Observations show that the rate at which this phenomenon develops differs highly from source to source, so that the time-integrated mass loss as a function of the initial conditions (mass, metallicity, etc.) and of the stage of evolution is presently not well understood. Aims. We are investigating the mass loss history of AGB stars by observing the molecular and atomic emissions of their circumstellar envelopes. Methods. In this work we have selected two stars that are on the thermally pulsing phase of the AGB (TP-AGB) and for which high quality data in the CO rotation lines and in the atomic hydrogen line at 21 cm could be obained. Results. V1942 Sgr, a carbon star of the Irregular variability type, shows a complex CO line profile that may originate from a long-lived wind at a rate of ~ 10^-7 Msol/yr, and from a young (< 10^4 years) fast outflow at a rate of ~ 5 10^-7 Msol/yr. Intense HI emission indicates a detached shell with 0.044 Msol of hydrogen. This shell probably results from the slowing-down, by surrounding matter, of the same long-lived wind observed in CO that has been active during ~ 6 10^5 years. On the other hand, the carbon Mira V CrB is presently undergoing mass loss at a rate of 2 10^-7 Msol/yr, but was not detected in HI. The wind is mostly molecular, and was active for at most 3 10^4 years, with an integrated mass loss of at most 6.5 10^-3 Msol. Conclusions. Although both sources are carbon stars on the TP-AGB, they appear to develop mass loss under very different conditions, and a high rate of mass loss may not imply a high integrated mass loss.Comment: Accepted for publication in Astron. Astrophy

Laser spectroscopy of hyperfine structure in highly-charged ions: a test of QED at high fields

An overview is presented of laser spectroscopy experiments with cold, trapped, highly-charged ions, which will be performed at the HITRAP facility at GSI in Darmstadt (Germany). These high-resolution measurements of ground state hyperfine splittings will be three orders of magnitude more precise than previous measurements. Moreover, from a comparison of measurements of the hyperfine splittings in hydrogen- and lithium-like ions of the same isotope, QED effects at high electromagnetic fields can be determined within a few percent. Several candidate ions suited for these laser spectroscopy studies are presented.Comment: 5 pages, 1 figure, 1 table. accepted for Canadian Journal of Physics (2006

Role of signal averaging of the surface QRS complex in selecting patients with nonsustained ventricular tachycardia and high grade ventricular arrhythmias for programmed ventricular stimulation

Signal averaging of the surface QRS complex was performed before programmed ventricular stimulation in 53 individuals with high grade ventricular arrhythmias or nonsustained ventricular tachycardia, or both. An abnormal signal-averaged electrocardiogram (ECG) was recorded in 22 patients and was associated with inducible ventricular tachycardia in 12 (55%) of the 22. In contrast, a normal signal-averaged ECG was associated with inducible tachycardia in only 1 (3%) of 31 individuals (p < 0.005). The group with inducible tachycardia had a longer duration of the signal-averaged QRS complex (124 ± 19 versus 96 ± 26 ms) and of low amplitude signals (44 ± 13 versus 29 ± 11 ms) (p < 0.005). In addition, the root mean square voltage of the terminal 40 ms was lower in this group (20 ± 14 versus 48 ± 34 µV, p < 0.005).Twenty-seven of the 53 subjects had a prior myocardial infarction; 17 (63%) of the 27 had an abnormal signalaveraged ECG, and ventricular tachycardia was inducible in 10 (59%) of the 17. A normal signal-averaged ECG was recorded in 10 of the 27 patients and only 1 (10%) of these 10 had inducible tachycardia. An abnormal signal-averaged ECG had a 91% sensitivity and a 56% specificity with respect to subsequent induction of tachycardia.During long-term follow-up, 2 (15%) of the 13 patients with inducible ventricular tachycardia who were treated with eleetrophysiologically guided antiarrhythmics therapy died suddenly; the remaining 11 patients (85%) are alive 15 ± 10 months after electrophysiologic testing. Both of these patients who died had an abnormal signal-averaged ECG. In contrast, only 2 (5%) of the 40 patients with no inducible tachycardia, both with a normal signal-averaged ECG, have had an arrhythmic event; the other 38 patients have remained free of sustained ventricular arrhythmia for a follow-up period of 17 ± 9 months.In conclusion: 1) Signal averaging of the surface QRS complex is useful in identifying patients with nonsustained ventricular tachycardia or high grade ventricular arrhythmias, or both, who will have inducible ventricular tachycardia on programmed ventricular stimulation. 2) Inducibility of arrhythmia is unlikely in individuals who have a normal signal-averaged ECG despite the presence of complex ventricular arrhythmia. 3) The occurrence of spontaneous sustained ventricular tachyarrhythmias is low in patients with a prior myocardial infarction and without inducible ventricular tachycardia who have nonsustained ventricular tachycardia or complex ventricular arrhythmias and a normal signal-averaged ECG. 4) Signalaveraged electrocardiography may be useful in detecting low risk groups of patients with complex ventricular arrhythmias who do not require electrophysiologic testing or antiarrhythmic therapy

Duration learning for analysis of nanopore ionic current blockades

<p>Abstract</p> <p>Background</p> <p>Ionic current blockade signal processing, for use in nanopore detection, offers a promising new way to analyze single molecule properties, with potential implications for DNA sequencing. The alpha-Hemolysin transmembrane channel interacts with a translocating molecule in a nontrivial way, frequently evidenced by a complex ionic flow blockade pattern. Typically, recorded current blockade signals have several levels of blockade, with various durations, all obeying a fixed statistical profile for a given molecule. Hidden Markov Model (HMM) based duration learning experiments on artificial two-level Gaussian blockade signals helped us to identify proper modeling framework. We then apply our framework to the real multi-level DNA hairpin blockade signal.</p> <p>Results</p> <p>The identified upper level blockade state is observed with durations that are geometrically distributed (consistent with an a physical decay process for remaining in any given state). We show that mixture of convolution chains of geometrically distributed states is better for presenting multimodal long-tailed duration phenomena. Based on learned HMM profiles we are able to classify 9 base-pair DNA hairpins with accuracy up to 99.5% on signals from same-day experiments.</p> <p>Conclusion</p> <p>We have demonstrated several implementations for <it>de novo </it>estimation of duration distribution probability density function with HMM framework and applied our model topology to the real data. The proposed design could be handy in molecular analysis based on nanopore current blockade signal.</p

The NTD Nanoscope: potential applications and implementations

<p>Abstract</p> <p>Background</p> <p>Nanopore transduction detection (NTD) offers prospects for a number of highly sensitive and discriminative applications, including: (i) single nucleotide polymorphism (SNP) detection; (ii) targeted DNA re-sequencing; (iii) protein isoform assaying; and (iv) biosensing via antibody or aptamer coupled molecules. Nanopore event transduction involves single-molecule biophysics, engineered information flows, and nanopore cheminformatics. The NTD Nanoscope has seen limited use in the scientific community, however, due to lack of information about potential applications, and lack of availability for the device itself. Meta Logos Inc. is developing both pre-packaged device platforms and component-level (unassembled) kit platforms (the latter described here). In both cases a lipid bi-layer workstation is first established, then augmentations and operational protocols are provided to have a nanopore transduction detector. In this paper we provide an overview of the NTD Nanoscope applications and implementations. The NTD Nanoscope Kit, in particular, is a component-level reproduction of the standard NTD device used in previous research papers.</p> <p>Results</p> <p>The NTD Nanoscope method is shown to functionalize a single nanopore with a channel current modulator that is designed to transduce events, such as binding to a specific target. To expedite set-up in new lab settings, the calibration and troubleshooting for the NTD Nanoscope kit components and signal processing software, the NTD Nanoscope Kit, is designed to include a set of test buffers and control molecules based on experiments described in previous NTD papers (the model systems briefly described in what follows). The description of the Server-interfacing for advanced signal processing support is also briefly mentioned.</p> <p>Conclusions</p> <p>SNP assaying, SNP discovery, DNA sequencing and RNA-seq methods are typically limited by the accuracy of the error rate of the enzymes involved, such as methods involving the polymerase chain reaction (PCR) enzyme. The NTD Nanoscope offers a means to obtain higher accuracy as it is a single-molecule method that does not inherently involve use of enzymes, using a functionalized nanopore instead.</p

The Low- and Intermediate-Mass Stellar Population in the Small Magellanic Cloud: The Central Stars of Planetary Nebulae

We present a study on the central stars (CSs) of Planetary Nebulae (PNe) observed in the Small Magellanic Cloud (SMC) with the Space Telescope Imaging Spectrograph instrument on-board the HST. The stellar magnitudes have been measured using broad-band photometry, and Zanstra analysis of the nebulae provided the stellar temperatures. From the location of the CSs on the HR diagram, and by comparing the observed CSs with current models of stellar evolution, we infer the CSs masses. We examine closely the possibility of light contamination in the bandpass from an unrecognized stellar companion, and we establish strong constraints on the existence and nature of any binary companion. We find an average mass of 0.63 Msun, which is similar to the mass obtained for a sample of CSs in the LMC (0.65 Msun). However, the SMC and LMC CS mass distributions differ slightly, the SMC sample lacking an intermediate-mass stellar population (0.65 to 0.75 Msun). We discuss the significance and possible reasons for the difference between the two mass distributions. In particular, we consider the differences in the star formation history between the clouds and the mass-loss rate dependence on metallicity.Comment: 30 pages, 6 figures, 5 tables. To be published in ApJ (October 20