92 research outputs found
Stimulation with Peptidoglycan induces interleukin 6 and TLR2 expression and a concomitant downregulation of expression of adiponectin receptors 1 and 2 in 3T3-L1 adipocytes
<p>Abstract</p> <p>Background</p> <p>Inflammation is a major component of obesity and diabetes, and toll-like receptors (TLRs) play critical roles in the regulation of inflammation and response to pathogen associated molecular patterns (PAMPs) and fatty acids in. Although immune cells such as macrophages are primarily responsible for recognition and clearance of pathogens, adipocytes are also closely involved in the regulation of innate immunity and inflammation. Whereas it has been demonstrated that adipocytes respond to TLR4 stimulation with lipopolysacccharide, very little is known about their response to the TLR2 agonist, peptidoglycan.</p> <p>Methods</p> <p>We investigated the response to peptidoglycan from <it>Staphylococcus aureus </it>in differentiated 3T3-L1 adipocytes. Real-time PCR analysis was used to quantify the expression of interleukin 6 (IL6), adiponectin receptors (adipoR1 and adipoR2), toll-like receptor 2 (TLR2) and 4 (TLR2 4). Media level of IL6 was determined with ELISA.</p> <p>Results</p> <p>Adipocyte stimulation peptidoglycan induces IL6 expression (P < 0.01). Both siRNA mediated suppression of TLR2 and immunoneutralization of TLR2 with a TLR2 specific antibody inhibited response to peptidoglycan (P < 0.05). We also examined the regulation of TLR2 and TLR4 mRNA in peptidoglycan treated cells. Both peptidoglycan and lipopolysaccharide (LPS) robustly induce TLR2 mRNA expression, whereas TLR4 mRNA is weakly induced by LPS only (P < 0.05). Additionally, peptidoglycan downregulates the mRNA expression of adiponectin receptors, adipoR1 and adipoR2 (P < 0.05).</p> <p>Conclusion</p> <p>Obesity and type 2 diabetes are associated with increased expression of TLR2, this receptor could play a significant but previously unrecognized role in the establishment of chronic inflammation in adipose tissue in obesity.</p
High-power broadband laser source tunable from 3.0 um to 4.4 um based on a femtosecond Yb:fiber oscillator
We describe a tunable broadband mid-infrared laser source based on
difference-frequency mixing of a 100 MHz femtosecond Yb:fiber laser oscillator
and a Raman-shifted soliton generated with the same laser. The resulting light
is tunable over 3.0 um to 4.4 um, with a FWHM bandwidth of 170 nm and maximum
average output power up to 125 mW. The noise and coherence properties of this
source are also investigated and described.Comment: To appear in Optics Letter
The Solar Neighborhood. XXXIV. A Search for Planets Orbiting Nearby M Dwarfs using Astrometry
Astrometric measurements are presented for seven nearby stars with previously
detected planets: six M dwarfs (GJ 317, GJ 667C, GJ 581, GJ 849, GJ 876, and GJ
1214) and one K dwarf (BD 10 3166). Measurements are also presented for six
additional nearby M dwarfs without known planets, but which are more favorable
to astrometric detections of low mass companions, as well as three binary
systems for which we provide astrometric orbit solutions. Observations have
baselines of three to thirteen years, and were made as part of the RECONS
long-term astrometry and photometry program at the CTIO/SMARTS 0.9m telescope.
We provide trigonometric parallaxes and proper motions for all 16 systems, and
perform an extensive analysis of the astrometric residuals to determine the
minimum detectable companion mass for the 12 M dwarfs not having close stellar
secondaries. For the six M dwarfs with known planets, we are not sensitive to
planets, but can rule out the presence of all but the least massive brown
dwarfs at periods of 2 - 12 years. For the six more astrometrically favorable M
dwarfs, we conclude that none have brown dwarf companions, and are sensitive to
companions with masses as low as 1 for periods longer than two years.
In particular, we conclude that Proxima Centauri has no Jovian companions at
orbital periods of 2 - 12 years. These results complement previously published
M dwarf planet occurrence rates by providing astrometrically determined upper
mass limits on potential super-Jupiter companions at orbits of two years and
longer. As part of a continuing survey, these results are consistent with the
paucity of super-Jupiter and brown dwarf companions we find among the over 250
red dwarfs within 25 pc observed longer than five years in our astrometric
program.Comment: 18 pages, 5 figures, 4 tables, accepted for publication in A
Observations of Binary Stars with the Differential Speckle Survey Instrument. VII. Measures from 2010 September to 2012 February at the WIYN Telescope
We report on speckle observations of binary stars carried out at the WIYN
Telescope over the period from September 2010 through February 2012, providing
relative astrometry for 2521 observations of 883 objects, 856 of which are
double stars and 27 of which are triples. The separations measured span a range
of 0.01 to 1.75 arc seconds. Wavelengths of 562 nm, 692 nm, and 880 nm were
used, and differential photometry at one or more of these wavelengths is
presented in most cases. Sixty-six components were resolved for the first time.
We also estimate detection limits at 0.2 and 1.0 arc seconds for high-quality
observations in cases where no companion was seen, a total of 176 additional
objects. Detection limits vary based on observing conditions and
signal-to-noise ratio, but are approximately 4 magnitudes at 0.2 arc seconds
and 6 magnitudes at 1.0 arc seconds on average. Analyzing the measurement
precision of the data set, we find that the individual separations obtained
have linear measurement uncertainties of approximately 2 mas, and photometry is
uncertain to approximately 0.1 magnitudes in general. This work provides
fundamental, well-calibrated data for future orbit and mass determinations, and
we present three first orbits and total mass estimates of nearby K-dwarf
systems as examples of this potential
Lightning Reporting at 45th Weather Squadron: Recent Improvements
The 45th Weather Squadron (45 WS) provides daily lightning reports to space launch customers at CCAFS/KSC. These reports are provided to assess the need to inspect the electronics of satellite payloads, space launch vehicles, and ground support equipment for induced current damage from nearby lightning strokes. The 45 WS has made several improvements to the lightning reports during 2008-2009. The 4DLSS, implemented in April 2008, provides all lightning strokes as opposed to just one stroke per flash as done by the previous system. The 45 WS discovered that the peak current was being truncated to the nearest kilo amp in the database used to generate the daily lightning reports, which led to an up to 4% underestimate in the peak current for average lightning. This error was corrected and led to elimination of this underestimate. The 45 WS and their mission partners developed lightning location error ellipses for 99% and 95% location accuracies tailored to each individual stroke and began providing them in the spring of 2009. The new procedure provides the distance from the point of interest to the best location of the stroke (the center of the error ellipse) and the distance to the closest edge of the ellipse. This information is now included in the lightning reports, along with the peak current of the stroke. The initial method of calculating the error ellipses could only be used during normal duty hours, i.e. not during nights, weekends, or holidays. This method was improved later to provide lightning reports in near real-time, 24/7. The calculation of the distance to the closest point on the ellipse was also significantly improved later. Other improvements were also implemented. A new method to calculate the probability of any nearby lightning stroke. being within any radius of any point of interest was developed and is being implemented. This may supersede the use of location error ellipses. The 45 WS is pursuing adding data from nine NLDN sensors into 4DLSS in real-time. This will overcome the problem of 4DLSS missing some of the strong local strokes. This will also improve the location accuracy, reduce the size and eccentricity of the location error ellipses, and reduce the probability of nearby strokes being inside the areas of interest when few of the 4DLSS sensors are used in the stroke solution. This will not reduce 4DLSS performance when most of the 4DLSS sensors are used in the stroke solution. Finally, several possible future improvements were discussed, especially for improving the peak current estimate and the error estimate for peak current, and upgrading the 4DLSS. Some possible approaches for both of these goals were discussed
The Solar Neighborhood XXIV. Parallax Results from the CTIOPI 0.9-m Program: Stars with 1\farcs0 yr (MOTION Sample) and Subdwarfs
We present 41 trigonometric parallaxes of 37 stellar systems, most of which
have proper motions greater than 1\farcs0 yr. These are the first
trigonometric parallaxes for 24 systems. Overall, there are 15 red dwarf
systems and 22 red subdwarf systems in the sample. Five of the systems are
multiples with directly detected companions, and we have discovered
perturbations caused by unseen companions in two additional cases, the dwarf
LHS 501 and the subdwarf LHS 440. The latter system may eventually provide
important dynamical mass points on the subdwarf mass-luminosity relation. Two
additional stars of note are LHS 272, the third closest M-type subdwarf at a
distance of only 13.6 pc, and LHS 2734AB, a high velocity subdwarf binary with
700 km/sec, which likely exceeds the escape velocity of the Milky
Way. We also report the first long term variability study of cool subdwarfs
indicating that cool subdwarfs are less photometrically variable than their
main sequence counterparts.Comment: 28 pages, 7 figures, 3 tables, accepted by A
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